Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases

Bibliographic Details
Main Author: Costa, Monica
Publication Date: 2022
Other Authors: Pio, Luís Bernardo, Bule, Pedro, Duarte, Marlene, Alfaia, Cristina, Coelho, Diogo, Bras, Joana, Fontes, Carlos M.G.A., Prates, José A.M, Cardoso, Vânia
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10400.5/24781
Summary: Research Areas: Agriculture; Veterinary Sciences
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spelling Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyasesMacroalgaUlva lactucaCarbohydrate-active enzymeUlvan lyaseCell wall degradationResearch Areas: Agriculture; Veterinary SciencesABSTRACT - Green macroalgae, e.g., Ulva lactuca, are valuable bioactive sources of nutrients; but algae recalcitrant cell walls, composed of a complex cross-linked matrix of polysaccharides, can compromise their utilization as feedstuffs for monogastric animals. This study aimed to evaluate the ability of pre-selected Carbohy- drate-Active enZymes (CAZymes) and sulfatases to degrade U. lactuca cell walls and release nutritive compounds. A databank of 199 recombinant CAZymes and sulfatases was tested in vitro for their action towards U. lactuca cell wall polysaccharides. The enzymes were incubated with the macroalga, either alone or in combination, to release reducing sugars and decrease fluorescence intensity of Calcofluor White stained cell walls. The individual action of a polysaccharide lyase family 25 (PL25), an ulvan lyase, was shown to be the most efficient in cell wall disruption. The ulvan lyase treatment, in triplicate measures, promoted the release of 4.54 g/L (P < 0.001) reducing sugars, a mono- and oligosaccharides release of 11.4 and 11.2 mmol/100 g of dried alga (P < 0.01), respectively, and a decrease of 41.7% (P < 0.001) in cell wall fluorescence, in comparison to control. The ability of ulvan lyase treatment to promote the release of nutritional compounds from alga biomass was also evaluated. A release of some monounsaturated fatty acids was observed, particularly the health beneficial 18:1c9 (P < 0.001). How- ever, no significant release of total fatty acids (P > 0.05), proteins (P ¼ 0.861) or pigments (P > 0.05) was found. These results highlight the capacity of a single recombinant ulvan lyase (PL25 family) to incompletely disrupt U. lactuca cell walls. This enzyme could enhance the bioaccessibility of U. lactuca bioactive products with promising utilization in the feed industry.KEAI PUBLISHINGRepositório da Universidade de LisboaCosta, MonicaPio, Luís BernardoBule, PedroDuarte, MarleneAlfaia, CristinaCoelho, DiogoBras, JoanaFontes, Carlos M.G.A.Prates, José A.MCardoso, Vânia2022-07-07T17:10:33Z2022-062022-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/24781engCosta MM, Pio LB, Bule P, Cardoso VA, Duarte M, Alfaia CM, Coelho DF, Bras JA, Fontes CMGA, Prates JAM . 2022. Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases. Animal Nutrition, 9:p.184-192. DOI: https://doi.org/10.1016/j.aninu.2022.01.0042405-6383https://doi.org/10.1016/j.aninu.2022.01.0042405-6545info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T15:23:51Zoai:repositorio.ulisboa.pt:10400.5/24781Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T03:42:18.510061Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases
title Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases
spellingShingle Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases
Costa, Monica
Macroalga
Ulva lactuca
Carbohydrate-active enzyme
Ulvan lyase
Cell wall degradation
title_short Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases
title_full Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases
title_fullStr Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases
title_full_unstemmed Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases
title_sort Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases
author Costa, Monica
author_facet Costa, Monica
Pio, Luís Bernardo
Bule, Pedro
Duarte, Marlene
Alfaia, Cristina
Coelho, Diogo
Bras, Joana
Fontes, Carlos M.G.A.
Prates, José A.M
Cardoso, Vânia
author_role author
author2 Pio, Luís Bernardo
Bule, Pedro
Duarte, Marlene
Alfaia, Cristina
Coelho, Diogo
Bras, Joana
Fontes, Carlos M.G.A.
Prates, José A.M
Cardoso, Vânia
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Costa, Monica
Pio, Luís Bernardo
Bule, Pedro
Duarte, Marlene
Alfaia, Cristina
Coelho, Diogo
Bras, Joana
Fontes, Carlos M.G.A.
Prates, José A.M
Cardoso, Vânia
dc.subject.por.fl_str_mv Macroalga
Ulva lactuca
Carbohydrate-active enzyme
Ulvan lyase
Cell wall degradation
topic Macroalga
Ulva lactuca
Carbohydrate-active enzyme
Ulvan lyase
Cell wall degradation
description Research Areas: Agriculture; Veterinary Sciences
publishDate 2022
dc.date.none.fl_str_mv 2022-07-07T17:10:33Z
2022-06
2022-06-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/24781
url http://hdl.handle.net/10400.5/24781
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Costa MM, Pio LB, Bule P, Cardoso VA, Duarte M, Alfaia CM, Coelho DF, Bras JA, Fontes CMGA, Prates JAM . 2022. Recalcitrant cell wall of Ulva lactuca seaweed is degraded by a single ulvan lyase from family 25 of polysaccharide lyases. Animal Nutrition, 9:p.184-192. DOI: https://doi.org/10.1016/j.aninu.2022.01.004
2405-6383
https://doi.org/10.1016/j.aninu.2022.01.004
2405-6545
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv KEAI PUBLISHING
publisher.none.fl_str_mv KEAI PUBLISHING
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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