Genetic instability persists in non-neoplastic urothelial cells from patients with a history of urothelial cell carcinoma
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
| Texto Completo: | http://hdl.handle.net/10362/145289 |
Resumo: | 301079/2009-9, to D. M. F. S.) and Coordenação de Aperfeiçoamento de Pessoal de Nível superior (CAPES, fellowship to J. P. C. M.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
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Genetic instability persists in non-neoplastic urothelial cells from patients with a history of urothelial cell carcinomaGeneralSDG 3 - Good Health and Well-being301079/2009-9, to D. M. F. S.) and Coordenação de Aperfeiçoamento de Pessoal de Nível superior (CAPES, fellowship to J. P. C. M.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Bladder cancer is one of the most common genitourinary neoplasms in industrialized countries. Multifocality and high recurrence rates are prominent clinical features of this disease and contribute to its high morbidity. Therefore, more sensitive and less invasive techniques could help identify individuals with asymptomatic disease. In this context, we used the micronucleus assay to evaluate whether cytogenetic alterations could be used as biomarkers for monitoring patients with a history of urothelial cell carcinoma (UCC). We determined the frequency of micronucleated urothelial cells (MNC) in exfoliated bladder cells from 105 patients with (n = 52) or without (n = 53) a history of UCC, all of whom tested negative for neoplasia by cytopathological and histopathological analyses. MNC frequencies were increased in patients with a history of UCC (non-smoker and smoker/ex-smoker patients vs non-smoker and smoker/ex-smoker controls; p<0.001), in non-smoker UCC patients (vs non-smoker controls; p<0.01), and in smoker/ex-smoker controls (vs non-smoker controls; p<0.001). Patients with a history of recurrent disease also demonstrated a higher MNC frequency compared to patients with non-recurrent neoplasia. However, logistic regression using smoking habits, age and gender as confounding factors did not confirm MNC frequency as a marker for UCC recurrence. Fluorescent in situ hybridization analysis (using a pan-centromeric probe) showed that micronuclei (MN) arose mainly from clastogenic events regardless of UCC and/or smoking histories. In conclusion, our results confirm previous indications that subjects with a history of UCC harbor genetically unstable cells in the bladder urothelium. Furthermore, these results support using the micronucleus assay as an important tool for monitoring patients with a history of UCC and tumor recurrence.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)RUNMarcondes, João Paulo De CastroDe Oliveira, Maria Luiza Cotrim SartorGontijo, Alisson M.De Camargo, João Lauro VianaSalvadori, Daisy Maria Fávero2022-11-07T22:11:06Z2014-01-222014-01-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/145289eng1932-6203PURE: 47610851https://doi.org/10.1371/journal.pone.0086162info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T18:06:26Zoai:run.unl.pt:10362/145289Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:37:02.215989Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse |
| dc.title.none.fl_str_mv |
Genetic instability persists in non-neoplastic urothelial cells from patients with a history of urothelial cell carcinoma |
| title |
Genetic instability persists in non-neoplastic urothelial cells from patients with a history of urothelial cell carcinoma |
| spellingShingle |
Genetic instability persists in non-neoplastic urothelial cells from patients with a history of urothelial cell carcinoma Marcondes, João Paulo De Castro General SDG 3 - Good Health and Well-being |
| title_short |
Genetic instability persists in non-neoplastic urothelial cells from patients with a history of urothelial cell carcinoma |
| title_full |
Genetic instability persists in non-neoplastic urothelial cells from patients with a history of urothelial cell carcinoma |
| title_fullStr |
Genetic instability persists in non-neoplastic urothelial cells from patients with a history of urothelial cell carcinoma |
| title_full_unstemmed |
Genetic instability persists in non-neoplastic urothelial cells from patients with a history of urothelial cell carcinoma |
| title_sort |
Genetic instability persists in non-neoplastic urothelial cells from patients with a history of urothelial cell carcinoma |
| author |
Marcondes, João Paulo De Castro |
| author_facet |
Marcondes, João Paulo De Castro De Oliveira, Maria Luiza Cotrim Sartor Gontijo, Alisson M. De Camargo, João Lauro Viana Salvadori, Daisy Maria Fávero |
| author_role |
author |
| author2 |
De Oliveira, Maria Luiza Cotrim Sartor Gontijo, Alisson M. De Camargo, João Lauro Viana Salvadori, Daisy Maria Fávero |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Centro de Estudos de Doenças Crónicas (CEDOC) RUN |
| dc.contributor.author.fl_str_mv |
Marcondes, João Paulo De Castro De Oliveira, Maria Luiza Cotrim Sartor Gontijo, Alisson M. De Camargo, João Lauro Viana Salvadori, Daisy Maria Fávero |
| dc.subject.por.fl_str_mv |
General SDG 3 - Good Health and Well-being |
| topic |
General SDG 3 - Good Health and Well-being |
| description |
301079/2009-9, to D. M. F. S.) and Coordenação de Aperfeiçoamento de Pessoal de Nível superior (CAPES, fellowship to J. P. C. M.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
| publishDate |
2014 |
| dc.date.none.fl_str_mv |
2014-01-22 2014-01-22T00:00:00Z 2022-11-07T22:11:06Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10362/145289 |
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http://hdl.handle.net/10362/145289 |
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eng |
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eng |
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1932-6203 PURE: 47610851 https://doi.org/10.1371/journal.pone.0086162 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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RCAAP |
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Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
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Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia |
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info@rcaap.pt |
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