Growth of phototrophic biofilms from limestone monuments under laboratory conditions

Bibliographic Details
Main Author: Miller, Ana Zélia
Publication Date: 2009
Other Authors: Laiz, Leonila, Dionísio, Maria Amélia, Macedo, Maria Filomena, Saiz-Jimenez, Cesareo
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/4467
Summary: International Biodeterioration & Biodegradation,xxx (2009) 1–8
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spelling Growth of phototrophic biofilms from limestone monuments under laboratory conditionsPhototrophic microorganismsBiofilmLimestoneMonumentsDGGEInternational Biodeterioration & Biodegradation,xxx (2009) 1–8In the current study, five phototrophic biofilms from different Southern Europe limestone monuments were characterised by molecular techniques and cultivated under laboratory conditions. Phototrophic biofilms were collected from Orologio Tower in Martano (Italy), Santa Clara-a-Velha Monastery and Ajuda National Palace, both in Portugal, and Seville and Granada Cathedrals from Spain. The biofilms were grown under laboratory conditions and periodically sampled in order to monitor their evolution over a three-month period. Prokaryotic communities from natural samples and cultivated biofilms were monitored using denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA gene fragments in conjunction with clone sequencing and phylogenetic analysis. DNA-based molecular analysis of 16S rRNA gene fragments from the natural green biofilms revealed complex and different communities composition with respect to phototrophic microorganisms. The biofilms from Orologio Tower (Martano,Italy) and Santa Clara-a-Velha Monastery (Coimbra, Portugal) were dominated by the microalga Chlorella. The cyanobacterium Chroococcidiopsis was the dominating genus from Ajuda National Palace biofilm(Lisbon, Portugal). The biofilms from Seville and Granada Cathedrals (Spain) were both dominated by the cyanobacterium Pleurocapsa. The DGGE analysis of the cultivated biofilms showed that the communities developed differently in terms of species establishment and community composition during the threemonth incubation period. The biofilm culture from Coimbra (Portugal) showed a remarkable stability of the microbial components of the natural community in laboratory conditions. With this work,a multiple-species community assemblage was obtained for further stone colonisation experiments.Elsevier Ltd.RUNMiller, Ana ZéliaLaiz, LeonilaDionísio, Maria AméliaMacedo, Maria FilomenaSaiz-Jimenez, Cesareo2010-12-10T15:33:15Z20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/4467engMiller, A.Z., et al., Growth of phototrophic biofilms from limestone monuments under laboratory conditions, International Biodeterioration & Biodegradation (2009)0964-8305info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:07:50Zoai:run.unl.pt:10362/4467Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:38:36.951628Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Growth of phototrophic biofilms from limestone monuments under laboratory conditions
title Growth of phototrophic biofilms from limestone monuments under laboratory conditions
spellingShingle Growth of phototrophic biofilms from limestone monuments under laboratory conditions
Miller, Ana Zélia
Phototrophic microorganisms
Biofilm
Limestone
Monuments
DGGE
title_short Growth of phototrophic biofilms from limestone monuments under laboratory conditions
title_full Growth of phototrophic biofilms from limestone monuments under laboratory conditions
title_fullStr Growth of phototrophic biofilms from limestone monuments under laboratory conditions
title_full_unstemmed Growth of phototrophic biofilms from limestone monuments under laboratory conditions
title_sort Growth of phototrophic biofilms from limestone monuments under laboratory conditions
author Miller, Ana Zélia
author_facet Miller, Ana Zélia
Laiz, Leonila
Dionísio, Maria Amélia
Macedo, Maria Filomena
Saiz-Jimenez, Cesareo
author_role author
author2 Laiz, Leonila
Dionísio, Maria Amélia
Macedo, Maria Filomena
Saiz-Jimenez, Cesareo
author2_role author
author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Miller, Ana Zélia
Laiz, Leonila
Dionísio, Maria Amélia
Macedo, Maria Filomena
Saiz-Jimenez, Cesareo
dc.subject.por.fl_str_mv Phototrophic microorganisms
Biofilm
Limestone
Monuments
DGGE
topic Phototrophic microorganisms
Biofilm
Limestone
Monuments
DGGE
description International Biodeterioration & Biodegradation,xxx (2009) 1–8
publishDate 2009
dc.date.none.fl_str_mv 2009
2009-01-01T00:00:00Z
2010-12-10T15:33:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/4467
url http://hdl.handle.net/10362/4467
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Miller, A.Z., et al., Growth of phototrophic biofilms from limestone monuments under laboratory conditions, International Biodeterioration & Biodegradation (2009)
0964-8305
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier Ltd.
publisher.none.fl_str_mv Elsevier Ltd.
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833596080705503232

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