Investigation of the genotoxicity of digested titanium dioxide nanomaterials in human intestinal cells

Detalhes bibliográficos
Autor(a) principal: Vieira, Adriana
Data de Publicação: 2022
Outros Autores: Vital, Nádia, Rolo, Dora, Roque, Rossana, Gonçalves, Lídia M., Bettencourt, Ana, Silva, Maria João, Louro, Henriqueta
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: http://hdl.handle.net/10362/133484
Resumo: Funding: This work was funded by national funds through the FCT - Foundation for Science and Technology, I.P., under the project PTDC/SAUPUB/29481/2017. Research co-funded by UIDB/00009/2020; UIDP/ 00009/2020 (Centre for Toxicogenomics and Human Health – ToxOmics, FCT- Foundation for Science and Technology). NV holds a FCT PhD Scholarship grant (2020.07168.BD). iMed.ULisboa (UIDB/04138/ 2020 and UIDP/04138/2020) principal investigator grants CEECIND/ 03143/2017 (L. M. Gonçalves). The authors thank all the support from the colleagues Paula Alvito, Carla Martins and Ricardo Assunç˜ ao (Food Safety Department, INSA, Lisbon, Portugal) as well from all INGESTnano team members.
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spelling Investigation of the genotoxicity of digested titanium dioxide nanomaterials in human intestinal cellsGenotoxicityIn vitro simulated digestionIntestinal epithelial cellsNanomaterialsTitanium DioxideFood ScienceToxicologySDG 3 - Good Health and Well-beingFunding: This work was funded by national funds through the FCT - Foundation for Science and Technology, I.P., under the project PTDC/SAUPUB/29481/2017. Research co-funded by UIDB/00009/2020; UIDP/ 00009/2020 (Centre for Toxicogenomics and Human Health – ToxOmics, FCT- Foundation for Science and Technology). NV holds a FCT PhD Scholarship grant (2020.07168.BD). iMed.ULisboa (UIDB/04138/ 2020 and UIDP/04138/2020) principal investigator grants CEECIND/ 03143/2017 (L. M. Gonçalves). The authors thank all the support from the colleagues Paula Alvito, Carla Martins and Ricardo Assunç˜ ao (Food Safety Department, INSA, Lisbon, Portugal) as well from all INGESTnano team members.The widespread use of titanium dioxide nanomaterials (TiO2 NMs) in food and consumer products such as toothpaste or food contact materials, suggests the relevance of human oral exposure to these nanomaterials (NMs) and raises the possibility of adverse effects in the gastrointestinal tract (GIT). We previously showed that the in vitro digestion of TiO2 NMs may increase their toxicity in intestinal cells. In this work, we analyzed the genotoxicity and the intracellular reactive oxygen species induction by physiologically relevant concentrations of three different TiO2 NMs (NM-102, NM-103 and NM-105) in Caco-2 and HT29-MTX-E12 intestinal cells, while considering the potential influence of the digestion process in the NMs’ physiochemical characteristics. The results evidenced a DNA-damaging effect dependent on the NM, more relevant for the rutile/anatase NM-105, possibly due to its lower hydrodynamic size in the cells medium. In addition, the results of the micronucleus assay suggest effects on chromosomal integrity, an indicator of cancer risk, in the HT29-MTX-E12 cells, for all the tested TiO2 NMs, especially after the in vitro digestion. This work supports the evidence for concerns on the use of TiO2 NMs as a food additive, recently reported by EFSA, and for their use in applications in consumer products that may drive human exposure through ingestion.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centre for Toxicogenomics and Human Health (ToxOmics)RUNVieira, AdrianaVital, NádiaRolo, DoraRoque, RossanaGonçalves, Lídia M.Bettencourt, AnaSilva, Maria JoãoLouro, Henriqueta2022-02-23T23:17:37Z2022-032022-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/133484eng0278-6915PURE: 41736201https://doi.org/10.1016/j.fct.2022.112841info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:59:45Zoai:run.unl.pt:10362/133484Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:30:42.997949Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Investigation of the genotoxicity of digested titanium dioxide nanomaterials in human intestinal cells
title Investigation of the genotoxicity of digested titanium dioxide nanomaterials in human intestinal cells
spellingShingle Investigation of the genotoxicity of digested titanium dioxide nanomaterials in human intestinal cells
Vieira, Adriana
Genotoxicity
In vitro simulated digestion
Intestinal epithelial cells
Nanomaterials
Titanium Dioxide
Food Science
Toxicology
SDG 3 - Good Health and Well-being
title_short Investigation of the genotoxicity of digested titanium dioxide nanomaterials in human intestinal cells
title_full Investigation of the genotoxicity of digested titanium dioxide nanomaterials in human intestinal cells
title_fullStr Investigation of the genotoxicity of digested titanium dioxide nanomaterials in human intestinal cells
title_full_unstemmed Investigation of the genotoxicity of digested titanium dioxide nanomaterials in human intestinal cells
title_sort Investigation of the genotoxicity of digested titanium dioxide nanomaterials in human intestinal cells
author Vieira, Adriana
author_facet Vieira, Adriana
Vital, Nádia
Rolo, Dora
Roque, Rossana
Gonçalves, Lídia M.
Bettencourt, Ana
Silva, Maria João
Louro, Henriqueta
author_role author
author2 Vital, Nádia
Rolo, Dora
Roque, Rossana
Gonçalves, Lídia M.
Bettencourt, Ana
Silva, Maria João
Louro, Henriqueta
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Centre for Toxicogenomics and Human Health (ToxOmics)
RUN
dc.contributor.author.fl_str_mv Vieira, Adriana
Vital, Nádia
Rolo, Dora
Roque, Rossana
Gonçalves, Lídia M.
Bettencourt, Ana
Silva, Maria João
Louro, Henriqueta
dc.subject.por.fl_str_mv Genotoxicity
In vitro simulated digestion
Intestinal epithelial cells
Nanomaterials
Titanium Dioxide
Food Science
Toxicology
SDG 3 - Good Health and Well-being
topic Genotoxicity
In vitro simulated digestion
Intestinal epithelial cells
Nanomaterials
Titanium Dioxide
Food Science
Toxicology
SDG 3 - Good Health and Well-being
description Funding: This work was funded by national funds through the FCT - Foundation for Science and Technology, I.P., under the project PTDC/SAUPUB/29481/2017. Research co-funded by UIDB/00009/2020; UIDP/ 00009/2020 (Centre for Toxicogenomics and Human Health – ToxOmics, FCT- Foundation for Science and Technology). NV holds a FCT PhD Scholarship grant (2020.07168.BD). iMed.ULisboa (UIDB/04138/ 2020 and UIDP/04138/2020) principal investigator grants CEECIND/ 03143/2017 (L. M. Gonçalves). The authors thank all the support from the colleagues Paula Alvito, Carla Martins and Ricardo Assunç˜ ao (Food Safety Department, INSA, Lisbon, Portugal) as well from all INGESTnano team members.
publishDate 2022
dc.date.none.fl_str_mv 2022-02-23T23:17:37Z
2022-03
2022-03-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/133484
url http://hdl.handle.net/10362/133484
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0278-6915
PURE: 41736201
https://doi.org/10.1016/j.fct.2022.112841
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
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reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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