The influence of patterned nanofiber meshes on human mesenchymal stem cell osteogenesis

Bibliographic Details
Main Author: Martins, Albino
Publication Date: 2011
Other Authors: Silva, Marta L. Alves da, Faria, Susana, Marques, A. P., Reis, R. L., Neves, N. M.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/1822/12931
Summary: A specially designed electroconductive collector enables the electrospinning of P-NFM composed of areas of parallel/uniaxially aligned fibers and areas of random/orthogonal nanofiber distribution. The biological relevance of P-NFM is demonstrated using hBMSCs as an autologous cell source. The structures induce cell orientation along the uniaxially aligned fibers, mainly during earlier culturing periods under basal and osteogenic differentiation conditions. The microtopography of the P-NFM also controls the deposition of mineralized extracellular matrix along the pre-defined fiber direction. Genotypic characterization confirms the successful differentiation into the osteogenic lineage.
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spelling The influence of patterned nanofiber meshes on human mesenchymal stem cell osteogenesisBiodegradableElectrospinningMesenchymal stem cellsPatterned nanofiber meshesTissue engineeringScience & TechnologyA specially designed electroconductive collector enables the electrospinning of P-NFM composed of areas of parallel/uniaxially aligned fibers and areas of random/orthogonal nanofiber distribution. The biological relevance of P-NFM is demonstrated using hBMSCs as an autologous cell source. The structures induce cell orientation along the uniaxially aligned fibers, mainly during earlier culturing periods under basal and osteogenic differentiation conditions. The microtopography of the P-NFM also controls the deposition of mineralized extracellular matrix along the pre-defined fiber direction. Genotypic characterization confirms the successful differentiation into the osteogenic lineage.European Integrated Project GENOSTEM (LSH-STREP-CT-2003-503161); European Network of Excellence EXPERTISSUES (NMP3-CT-2004-500283); Portuguese Foundation for Science and Technology for the project Naturally Nano (POCI/EME/58982/2004) and the PhD grant SFRH/BD/24382/2005)WileyUniversidade do MinhoMartins, AlbinoSilva, Marta L. Alves daFaria, SusanaMarques, A. P.Reis, R. L.Neves, N. M.2011-07-012011-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/12931eng1616-518710.1002/mabi.20110001221485007http://onlinelibrary.wiley.com/doi/10.1002/mabi.201100012/abstractinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T04:27:21Zoai:repositorium.sdum.uminho.pt:1822/12931Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T14:48:42.785223Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv The influence of patterned nanofiber meshes on human mesenchymal stem cell osteogenesis
title The influence of patterned nanofiber meshes on human mesenchymal stem cell osteogenesis
spellingShingle The influence of patterned nanofiber meshes on human mesenchymal stem cell osteogenesis
Martins, Albino
Biodegradable
Electrospinning
Mesenchymal stem cells
Patterned nanofiber meshes
Tissue engineering
Science & Technology
title_short The influence of patterned nanofiber meshes on human mesenchymal stem cell osteogenesis
title_full The influence of patterned nanofiber meshes on human mesenchymal stem cell osteogenesis
title_fullStr The influence of patterned nanofiber meshes on human mesenchymal stem cell osteogenesis
title_full_unstemmed The influence of patterned nanofiber meshes on human mesenchymal stem cell osteogenesis
title_sort The influence of patterned nanofiber meshes on human mesenchymal stem cell osteogenesis
author Martins, Albino
author_facet Martins, Albino
Silva, Marta L. Alves da
Faria, Susana
Marques, A. P.
Reis, R. L.
Neves, N. M.
author_role author
author2 Silva, Marta L. Alves da
Faria, Susana
Marques, A. P.
Reis, R. L.
Neves, N. M.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Martins, Albino
Silva, Marta L. Alves da
Faria, Susana
Marques, A. P.
Reis, R. L.
Neves, N. M.
dc.subject.por.fl_str_mv Biodegradable
Electrospinning
Mesenchymal stem cells
Patterned nanofiber meshes
Tissue engineering
Science & Technology
topic Biodegradable
Electrospinning
Mesenchymal stem cells
Patterned nanofiber meshes
Tissue engineering
Science & Technology
description A specially designed electroconductive collector enables the electrospinning of P-NFM composed of areas of parallel/uniaxially aligned fibers and areas of random/orthogonal nanofiber distribution. The biological relevance of P-NFM is demonstrated using hBMSCs as an autologous cell source. The structures induce cell orientation along the uniaxially aligned fibers, mainly during earlier culturing periods under basal and osteogenic differentiation conditions. The microtopography of the P-NFM also controls the deposition of mineralized extracellular matrix along the pre-defined fiber direction. Genotypic characterization confirms the successful differentiation into the osteogenic lineage.
publishDate 2011
dc.date.none.fl_str_mv 2011-07-01
2011-07-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/12931
url http://hdl.handle.net/1822/12931
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1616-5187
10.1002/mabi.201100012
21485007
http://onlinelibrary.wiley.com/doi/10.1002/mabi.201100012/abstract
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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