Studies in the mouse model identify strain variability as a major determinant of disease outcome in Leishmania infantum infection

Bibliographic Details
Main Author: Marques, Filipe
Publication Date: 2015
Other Authors: Vale-Costa, Sílvia, Cruz, Tânia, Marques, Joana Moreira, Silva, Tânia, Neves, João Vilares, Cortes, Sofia, Fernandes, Ana, Rocha, Eduardo, Appelberg, Rui, Rodrigues, Pedro, Tomás, Ana M., Gomes, Maria Salomé
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: https://doi.org/10.1186/s13071-015-1259-6
Summary: PMID: 26684322 WOS:000367080800004
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spelling Studies in the mouse model identify strain variability as a major determinant of disease outcome in Leishmania infantum infectionParasitologyInfectious DiseasesSDG 3 - Good Health and Well-beingPMID: 26684322 WOS:000367080800004Background: Visceral leishmaniasis is a severe and potentially fatal disease caused by protozoa of the genus Leishmania, transmitted by phlebotomine sandflies. In Europe and the Mediterranean region, L. infantum is the commonest agent of visceral leishmaniasis, causing a wide spectrum of clinical manifestations, including asymptomatic carriage, cutaneous lesions and severe visceral disease. Visceral leishmaniasis is more frequent in immunocompromised individuals and data obtained in experimental models of infection have highlighted the importance of the host immune response, namely the efficient activation of host's macrophages, in determining infection outcome. Conversely, few studies have addressed a possible contribution of parasite variability to this outcome. Methods: In this study, we compared three isolates of L. infantum regarding their capacity to grow in the organs of mice, the way they activate the host's macrophages and other components of the immune response and also their capacity to cope with host's antimicrobial mechanisms, namely reactive oxygen and nitrogen species. Results: We found that the three parasite strains significantly differed regarding the degree to which they induced nitric oxide synthase (NOS2) and arginase expression in infected macrophages and the pattern of cytokine production they induced in the host, resulting in different degrees of inflammatory response in infected livers. Additionally, the three strains also significantly differed in their in vitro susceptibility to reactive oxygen and nitrogen species. This variability was reflected in the capacity of each strain to persist and proliferate in the organs of wild-type as well as NOS2- and phagocyte oxidase- deficient mice. Conclusions: The results obtained in this study show that parasite strain variability is an important determinant of disease outcome in L. infantum visceral leishmaniasis, with relevant implications for studies on host-pathogen interaction and also for leishmanicidal drug development.Global Health and Tropical Medicine (GHTM)Vector borne diseases and pathogens (VBD)Instituto de Higiene e Medicina Tropical (IHMT)RUNMarques, FilipeVale-Costa, SílviaCruz, TâniaMarques, Joana MoreiraSilva, TâniaNeves, João VilaresCortes, SofiaFernandes, AnaRocha, EduardoAppelberg, RuiRodrigues, PedroTomás, Ana M.Gomes, Maria Salomé2018-05-11T22:02:55Z2015-12-182015-12-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1186/s13071-015-1259-6engPURE: 1729028http://www.scopus.com/inward/record.url?scp=84950313576&partnerID=8YFLogxKhttps://doi.org/10.1186/s13071-015-1259-6info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:32:36Zoai:run.unl.pt:10362/36589Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:03:34.216191Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Studies in the mouse model identify strain variability as a major determinant of disease outcome in Leishmania infantum infection
title Studies in the mouse model identify strain variability as a major determinant of disease outcome in Leishmania infantum infection
spellingShingle Studies in the mouse model identify strain variability as a major determinant of disease outcome in Leishmania infantum infection
Marques, Filipe
Parasitology
Infectious Diseases
SDG 3 - Good Health and Well-being
title_short Studies in the mouse model identify strain variability as a major determinant of disease outcome in Leishmania infantum infection
title_full Studies in the mouse model identify strain variability as a major determinant of disease outcome in Leishmania infantum infection
title_fullStr Studies in the mouse model identify strain variability as a major determinant of disease outcome in Leishmania infantum infection
title_full_unstemmed Studies in the mouse model identify strain variability as a major determinant of disease outcome in Leishmania infantum infection
title_sort Studies in the mouse model identify strain variability as a major determinant of disease outcome in Leishmania infantum infection
author Marques, Filipe
author_facet Marques, Filipe
Vale-Costa, Sílvia
Cruz, Tânia
Marques, Joana Moreira
Silva, Tânia
Neves, João Vilares
Cortes, Sofia
Fernandes, Ana
Rocha, Eduardo
Appelberg, Rui
Rodrigues, Pedro
Tomás, Ana M.
Gomes, Maria Salomé
author_role author
author2 Vale-Costa, Sílvia
Cruz, Tânia
Marques, Joana Moreira
Silva, Tânia
Neves, João Vilares
Cortes, Sofia
Fernandes, Ana
Rocha, Eduardo
Appelberg, Rui
Rodrigues, Pedro
Tomás, Ana M.
Gomes, Maria Salomé
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Global Health and Tropical Medicine (GHTM)
Vector borne diseases and pathogens (VBD)
Instituto de Higiene e Medicina Tropical (IHMT)
RUN
dc.contributor.author.fl_str_mv Marques, Filipe
Vale-Costa, Sílvia
Cruz, Tânia
Marques, Joana Moreira
Silva, Tânia
Neves, João Vilares
Cortes, Sofia
Fernandes, Ana
Rocha, Eduardo
Appelberg, Rui
Rodrigues, Pedro
Tomás, Ana M.
Gomes, Maria Salomé
dc.subject.por.fl_str_mv Parasitology
Infectious Diseases
SDG 3 - Good Health and Well-being
topic Parasitology
Infectious Diseases
SDG 3 - Good Health and Well-being
description PMID: 26684322 WOS:000367080800004
publishDate 2015
dc.date.none.fl_str_mv 2015-12-18
2015-12-18T00:00:00Z
2018-05-11T22:02:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1186/s13071-015-1259-6
url https://doi.org/10.1186/s13071-015-1259-6
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PURE: 1729028
http://www.scopus.com/inward/record.url?scp=84950313576&partnerID=8YFLogxK
https://doi.org/10.1186/s13071-015-1259-6
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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