Using zeta-potential measurements to quantify peptide partition to lipid membranes
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
| Texto Completo: | http://hdl.handle.net/10451/6743 |
Resumo: | © The Author(s) 2011. This article is published with open access at Springerlink.com. |
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Using zeta-potential measurements to quantify peptide partition to lipid membranesPartition constantZeta-potentialFluorescenceMembraneAntimicrobial peptides© The Author(s) 2011. This article is published with open access at Springerlink.com.Open Access: This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.Many cellular phenomena occur on the biomembranes. There are plenty of molecules (natural or xenobiotics) that interact directly or partially with the cell membrane. Biomolecules, such as several peptides (e.g., antimicrobial peptides) and proteins, exert their effects at the cell membrane level. This feature makes necessary investigating their interactions with lipids to clarify their mechanisms of action and side effects necessary. The determination of molecular lipid/water partition constants (Kp) is frequently used to quantify the extension of the interaction. The determination of this parameter has been achieved by using different methodologies, such as UV-Vis absorption spectrophotometry, fluorescence spectroscopy and ζ-potential measurements. In this work, we derived and tested a mathematical model to determine the Kp from ζ-potential data. The values obtained with this method were compared with those obtained by fluorescence spectroscopy, which is a regular technique used to quantify the interaction of intrinsically fluorescent peptides with selected biomembrane model systems. Two antimicrobial peptides (BP100 and pepR) were evaluated by this new method. The results obtained by this new methodology show that ζ-potential is a powerful technique to quantify peptide/lipid interactions of a wide variety of charged molecules, overcoming some of the limitations inherent to other techniques, such as the need for fluorescent labeling.This work was partially supported by project PTDC/QUI/ 69937/2006 from Fundação para a Ciência e Tecnologia-Ministério da Ciência, Tecnologia e Ensino Superior (FCT-MCTES, Portugal), and by Fundação Calouste Gulbenkian (Portugal). JMF and MMD also thank FCT-MCTES for grants IMM/BT/37-2010 and SFRH/BD/41750/2007, respectively.SpringerRepositório da Universidade de LisboaFreire, João M.Domingues, Marco M.Matos, JoanaMelo, Manuel N.Veiga, Ana SaloméSantos, Nuno C.Castanho, Miguel A. R. B.2012-07-23T12:00:01Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/6743engEur Biophys J (2011) 40:481–4870175-7571http://dx.doi.org/10.1007/s00249-010-0661-4info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T12:53:52Zoai:repositorio.ulisboa.pt:10451/6743Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T02:30:22.962076Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse |
| dc.title.none.fl_str_mv |
Using zeta-potential measurements to quantify peptide partition to lipid membranes |
| title |
Using zeta-potential measurements to quantify peptide partition to lipid membranes |
| spellingShingle |
Using zeta-potential measurements to quantify peptide partition to lipid membranes Freire, João M. Partition constant Zeta-potential Fluorescence Membrane Antimicrobial peptides |
| title_short |
Using zeta-potential measurements to quantify peptide partition to lipid membranes |
| title_full |
Using zeta-potential measurements to quantify peptide partition to lipid membranes |
| title_fullStr |
Using zeta-potential measurements to quantify peptide partition to lipid membranes |
| title_full_unstemmed |
Using zeta-potential measurements to quantify peptide partition to lipid membranes |
| title_sort |
Using zeta-potential measurements to quantify peptide partition to lipid membranes |
| author |
Freire, João M. |
| author_facet |
Freire, João M. Domingues, Marco M. Matos, Joana Melo, Manuel N. Veiga, Ana Salomé Santos, Nuno C. Castanho, Miguel A. R. B. |
| author_role |
author |
| author2 |
Domingues, Marco M. Matos, Joana Melo, Manuel N. Veiga, Ana Salomé Santos, Nuno C. Castanho, Miguel A. R. B. |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Freire, João M. Domingues, Marco M. Matos, Joana Melo, Manuel N. Veiga, Ana Salomé Santos, Nuno C. Castanho, Miguel A. R. B. |
| dc.subject.por.fl_str_mv |
Partition constant Zeta-potential Fluorescence Membrane Antimicrobial peptides |
| topic |
Partition constant Zeta-potential Fluorescence Membrane Antimicrobial peptides |
| description |
© The Author(s) 2011. This article is published with open access at Springerlink.com. |
| publishDate |
2011 |
| dc.date.none.fl_str_mv |
2011 2011-01-01T00:00:00Z 2012-07-23T12:00:01Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10451/6743 |
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http://hdl.handle.net/10451/6743 |
| dc.language.iso.fl_str_mv |
eng |
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eng |
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Eur Biophys J (2011) 40:481–487 0175-7571 http://dx.doi.org/10.1007/s00249-010-0661-4 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Springer |
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Springer |
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