The diversity, metabolomics profiling, and the pharmacological potential of actinomycetes isolated from the estremadura spur pockmarks (Portugal)

Bibliographic Details
Main Author: Pinto-Almeida, António
Publication Date: 2021
Other Authors: Bauermeister, Anelize, Luppino, Luca, Grilo, Inês R., Oliveira, Juliana, Sousa, Joana R., Petras, Daniel, Rodrigues, Clara F., Prieto-Davó, Alejandra, Tasdemir, Deniz, Sobral, Rita G., Gaudêncio, Susana P.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/132128
Summary: LA/P/0140/2020 PTDC/QUIQUI/119116/2010 Decree-Law 57/2016, of 29 August, changed by Law 57/2017, of 19 July.
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spelling The diversity, metabolomics profiling, and the pharmacological potential of actinomycetes isolated from the estremadura spur pockmarks (Portugal)ActinobacteriaAntimicrobial activityBlue biotechnologyEstremadura Spur pockmarksMarine natural productsMarine-derived actinomycetesMetabolomicsMolecular networkingQemistreeDrug DiscoverySDG 14 - Life Below WaterLA/P/0140/2020 PTDC/QUIQUI/119116/2010 Decree-Law 57/2016, of 29 August, changed by Law 57/2017, of 19 July.The Estremadura Spur pockmarks are a unique and unexplored ecosystem located in the North Atlantic, off the coast of Portugal. A total of 85 marine-derived actinomycetes were isolated and cultured from sediments collected from this ecosystem at a depth of 200 to 350 m. Nine genera, Streptomyces, Micromonospora, Saccharopolyspora, Actinomadura, Actinopolymorpha, Nocardiopsis, Saccharomonospora, Stackebrandtia, and Verrucosispora were identified by 16S rRNA gene sequencing analyses, from which the first two were the most predominant. Non-targeted LC-MS/MS, in combination with molecular networking, revealed high metabolite diversity, including several known metabolites, such as surugamide, antimycin, etamycin, physostigmine, desferrioxamine, ikarugamycin, piericidine, and rakicidin derivatives, as well as numerous unidentified metabolites. Taxonomy was the strongest parameter influencing the metabolite production, highlighting the different biosynthetic potentials of phylogenetically related actinomycetes; the majority of the chemical classes can be used as chemotaxonomic markers, as the metabolite distribution was mostly genera-specific. The EtOAc extracts of the actinomycete isolates demonstrated antimicrobial and antioxidant activity. Altogether, this study demonstrates that the Estremadura Spur is a source of actinomycetes with potential applications for biotechnology. It highlights the importance of investigating actinomycetes from unique ecosystems, such as pockmarks, as the metabolite production reflects their adaptation to this habitat.UCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaRUNPinto-Almeida, AntónioBauermeister, AnelizeLuppino, LucaGrilo, Inês R.Oliveira, JulianaSousa, Joana R.Petras, DanielRodrigues, Clara F.Prieto-Davó, AlejandraTasdemir, DenizSobral, Rita G.Gaudêncio, Susana P.2022-02-02T23:15:49Z2021-12-232021-12-23T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/132128eng1660-3397PURE: 36538807https://doi.org/10.3390/md20010021info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:58:58Zoai:run.unl.pt:10362/132128Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:30:10.674175Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv The diversity, metabolomics profiling, and the pharmacological potential of actinomycetes isolated from the estremadura spur pockmarks (Portugal)
title The diversity, metabolomics profiling, and the pharmacological potential of actinomycetes isolated from the estremadura spur pockmarks (Portugal)
spellingShingle The diversity, metabolomics profiling, and the pharmacological potential of actinomycetes isolated from the estremadura spur pockmarks (Portugal)
Pinto-Almeida, António
Actinobacteria
Antimicrobial activity
Blue biotechnology
Estremadura Spur pockmarks
Marine natural products
Marine-derived actinomycetes
Metabolomics
Molecular networking
Qemistree
Drug Discovery
SDG 14 - Life Below Water
title_short The diversity, metabolomics profiling, and the pharmacological potential of actinomycetes isolated from the estremadura spur pockmarks (Portugal)
title_full The diversity, metabolomics profiling, and the pharmacological potential of actinomycetes isolated from the estremadura spur pockmarks (Portugal)
title_fullStr The diversity, metabolomics profiling, and the pharmacological potential of actinomycetes isolated from the estremadura spur pockmarks (Portugal)
title_full_unstemmed The diversity, metabolomics profiling, and the pharmacological potential of actinomycetes isolated from the estremadura spur pockmarks (Portugal)
title_sort The diversity, metabolomics profiling, and the pharmacological potential of actinomycetes isolated from the estremadura spur pockmarks (Portugal)
author Pinto-Almeida, António
author_facet Pinto-Almeida, António
Bauermeister, Anelize
Luppino, Luca
Grilo, Inês R.
Oliveira, Juliana
Sousa, Joana R.
Petras, Daniel
Rodrigues, Clara F.
Prieto-Davó, Alejandra
Tasdemir, Deniz
Sobral, Rita G.
Gaudêncio, Susana P.
author_role author
author2 Bauermeister, Anelize
Luppino, Luca
Grilo, Inês R.
Oliveira, Juliana
Sousa, Joana R.
Petras, Daniel
Rodrigues, Clara F.
Prieto-Davó, Alejandra
Tasdemir, Deniz
Sobral, Rita G.
Gaudêncio, Susana P.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DCV - Departamento de Ciências da Vida
RUN
dc.contributor.author.fl_str_mv Pinto-Almeida, António
Bauermeister, Anelize
Luppino, Luca
Grilo, Inês R.
Oliveira, Juliana
Sousa, Joana R.
Petras, Daniel
Rodrigues, Clara F.
Prieto-Davó, Alejandra
Tasdemir, Deniz
Sobral, Rita G.
Gaudêncio, Susana P.
dc.subject.por.fl_str_mv Actinobacteria
Antimicrobial activity
Blue biotechnology
Estremadura Spur pockmarks
Marine natural products
Marine-derived actinomycetes
Metabolomics
Molecular networking
Qemistree
Drug Discovery
SDG 14 - Life Below Water
topic Actinobacteria
Antimicrobial activity
Blue biotechnology
Estremadura Spur pockmarks
Marine natural products
Marine-derived actinomycetes
Metabolomics
Molecular networking
Qemistree
Drug Discovery
SDG 14 - Life Below Water
description LA/P/0140/2020 PTDC/QUIQUI/119116/2010 Decree-Law 57/2016, of 29 August, changed by Law 57/2017, of 19 July.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-23
2021-12-23T00:00:00Z
2022-02-02T23:15:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/132128
url http://hdl.handle.net/10362/132128
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1660-3397
PURE: 36538807
https://doi.org/10.3390/md20010021
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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