Chitosan nanofibers as scaffolds for peripheral nerve regeneration

Bibliographic Details
Main Author: Carvalho, C. R.
Publication Date: 2013
Other Authors: Silva-Correia, Joana, Martins, A., Oliveira, Joaquim M., Reis, R. L.
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/1822/26942
Summary: Publicado em : "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013)
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spelling Chitosan nanofibers as scaffolds for peripheral nerve regenerationChitosanNanofibersPeripheral Nerve RegenerationPublicado em : "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013)Damage to the Peripheral Nervous System (PNS) is remarkably common and occurs mainly from trauma or a complication of surgery [1]. Although recovery of nerve function occurs in some mild injuries, outcomes are frequently poor following severe trauma, resulting in long-term impairment of limb function, dysaesthesia and pain, often with associated psychological effects [2]. In contrast to the central nervous system, the PNS includes an intrinsic capacity to regenerate. Currently, the gold standard autograft repair of the damaged peripheral nerve is far from optimal and is often disappointing. The alternative to the use of grafts is the use of a scaffold that consists in an artificial nerve guide, namely a hollow tube, combined with engineered biomaterials filling its interior in order to provide topographical cues as it has been postulated that axon elongation requires guidance by contact with appropriate substrates. In the scope of the Biohybrid project, chitosan (CS) powders from Altakitin were supplied and its cytotoxicity was assessed. According to cell viability percentage, no cytotoxicity was observed. Using these biomedical grade powders, biodegradable scaffolds were developed to support neuronal regeneration using the electrospinning technique to produce nanofibers from chitosan solutions. Random and aligned nanofibers were produced and characterized, using techniques such as FTIR, SEM, AFM, DSC and contact angle. To produce a mesh of random chitosan fibers with no beads or other defects, a 5% chitosan solution in trifluoroacetic-acid and dicloromethane as solvents, in the proportion of 70:30 was used. They are super hydrophilic, defect-free and have an average diameter of 184±36nm. Yarns of aligned chitosan fibers were obtained using the two blades placed in line set up, with the exact same conditions used to obtain random nanofibers. In vitro tests to characterize L929 cells viability (MTS assay) and proliferation (DNA quantification) on nanofibers scaffold were performed.WileyUniversidade do MinhoCarvalho, C. R.Silva-Correia, JoanaMartins, A.Oliveira, Joaquim M.Reis, R. L.2013-102013-10-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/26942engCarvalho C. R., Silva-Correia J., Martins A., Oliveira J. M., Reis R. L. Chitosan nanofibers as scaffolds for peripheral nerve regeneration, TermStem 2013, doi:10.1002/term.1822, 2013info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T05:12:11Zoai:repositorium.sdum.uminho.pt:1822/26942Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:11:21.096866Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Chitosan nanofibers as scaffolds for peripheral nerve regeneration
title Chitosan nanofibers as scaffolds for peripheral nerve regeneration
spellingShingle Chitosan nanofibers as scaffolds for peripheral nerve regeneration
Carvalho, C. R.
Chitosan
Nanofibers
Peripheral Nerve Regeneration
title_short Chitosan nanofibers as scaffolds for peripheral nerve regeneration
title_full Chitosan nanofibers as scaffolds for peripheral nerve regeneration
title_fullStr Chitosan nanofibers as scaffolds for peripheral nerve regeneration
title_full_unstemmed Chitosan nanofibers as scaffolds for peripheral nerve regeneration
title_sort Chitosan nanofibers as scaffolds for peripheral nerve regeneration
author Carvalho, C. R.
author_facet Carvalho, C. R.
Silva-Correia, Joana
Martins, A.
Oliveira, Joaquim M.
Reis, R. L.
author_role author
author2 Silva-Correia, Joana
Martins, A.
Oliveira, Joaquim M.
Reis, R. L.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Carvalho, C. R.
Silva-Correia, Joana
Martins, A.
Oliveira, Joaquim M.
Reis, R. L.
dc.subject.por.fl_str_mv Chitosan
Nanofibers
Peripheral Nerve Regeneration
topic Chitosan
Nanofibers
Peripheral Nerve Regeneration
description Publicado em : "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013)
publishDate 2013
dc.date.none.fl_str_mv 2013-10
2013-10-01T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/26942
url http://hdl.handle.net/1822/26942
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Carvalho C. R., Silva-Correia J., Martins A., Oliveira J. M., Reis R. L. Chitosan nanofibers as scaffolds for peripheral nerve regeneration, TermStem 2013, doi:10.1002/term.1822, 2013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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