Partitioning of fibrinolytic protease from Bacillus sp. UFPEDA 485 by aqueous two-phase systems using PEG/sodium sulfate

Bibliographic Details
Main Author: Souza, F.A.S.D.
Publication Date: 2012
Other Authors: Nascimento, J.C.S., Sales, A. E., Silva, Nuno Miguel Sampaio Ribeiro Magalhães, Marcos, J. C., Porto, T. S, Teixeira, J. A., Porto, A. L. F.
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/1822/48386
Summary: XI Reunião Regional Nordeste da SBBq | 4th International Symposium in Biochemistry of Macromolecules and Biotechnology
id RCAP_cc8bd377ce9c707140b4a5e18c7bd81e
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/48386
network_acronym_str RCAP
network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository_id_str https://opendoar.ac.uk/repository/7160
spelling Partitioning of fibrinolytic protease from Bacillus sp. UFPEDA 485 by aqueous two-phase systems using PEG/sodium sulfateATPSPEG/Sodium SulfateFibrinolytic proteaseBacillusCiências Médicas::Biotecnologia MédicaXI Reunião Regional Nordeste da SBBq | 4th International Symposium in Biochemistry of Macromolecules and BiotechnologyThe fibrinolytic protease produced by bacteria of the genus Bacillus has attracted large interest in the pharmaceutical industry as a promising alternative in thrombolytic therapy due to their effectiveness in degrading fibrin, its production requiring the development of an efficient recovery process. Aqueous two-phase system (ATPS) have been recognized as an efficient and economical process for recovering enzymes due to their relative ease and low cost. The purpose of this work was to study the partition of fibrinolytic protease produced by Bacillus sp. UFPEDA 485 in a ATPS composed by Polyethylene glycol (PEG) and sodium sulfate using factorial design. The fibrinolytic protease production occurred in liquid culture medium containing 2% soy flour, pH 7.2, 150 rpm at 37 °C for 48 hours. To study the partitioning, was used a 23 full factorial design with four replicates at the central with the purpose of evaluating the effects and interactions of the independent variables: PEG molar mass (MMPEG), PEG concentration (CPEG) and sodium sulfate concentration (CNa2SO4) on the response variable: partition coefficient (K) of the fibrinolytic enzyme. In all the runs the enzyme partitioned to the top phase, indicating a significant interaction between the protein and the PEG. The best result was obtained at the central point, using MMPEG 6000 g/mol, CPEG 24 % and CNa2SO4 11,6 %. The partitions coefficients ranged between K = 327.63 and 2879.38. According to the results, the ATPS composed of PEG/sodium sulfate proved to be a promising method to extraction fibrinolytic protease.CAPES e CNPqinfo:eu-repo/semantics/publishedVersionUniversidade do MinhoSouza, F.A.S.D.Nascimento, J.C.S.Sales, A. E.Silva, Nuno Miguel Sampaio Ribeiro MagalhãesMarcos, J. C.Porto, T. STeixeira, J. A.Porto, A. L. F.2012-12-052012-12-05T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/48386engSouza, F.; Nascimento, J.; Sales, A. E.; Silva, N. M.; Marcos, J. C.; Porto, T. S; Teixeira, José A.; Porto, A. L. F., Partitioning of fibrinolytic protease from Bacillus sp. UFPEDA 485 by aqueous two-phase systems using PEG/sodium sulfate. XI Reunião Regional Nordeste da SBBq | 4th International Symposium in Biochemistry of Macromolecules and Biotechnology. Recife, Brazil, Dec 5-7, 2012.http://www.sbbq.org.br/v2/index.php?option=com_content&task=view&id=746info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T04:26:34Zoai:repositorium.sdum.uminho.pt:1822/48386Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T14:48:26.186249Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Partitioning of fibrinolytic protease from Bacillus sp. UFPEDA 485 by aqueous two-phase systems using PEG/sodium sulfate
title Partitioning of fibrinolytic protease from Bacillus sp. UFPEDA 485 by aqueous two-phase systems using PEG/sodium sulfate
spellingShingle Partitioning of fibrinolytic protease from Bacillus sp. UFPEDA 485 by aqueous two-phase systems using PEG/sodium sulfate
Souza, F.A.S.D.
ATPS
PEG/Sodium Sulfate
Fibrinolytic protease
Bacillus
Ciências Médicas::Biotecnologia Médica
title_short Partitioning of fibrinolytic protease from Bacillus sp. UFPEDA 485 by aqueous two-phase systems using PEG/sodium sulfate
title_full Partitioning of fibrinolytic protease from Bacillus sp. UFPEDA 485 by aqueous two-phase systems using PEG/sodium sulfate
title_fullStr Partitioning of fibrinolytic protease from Bacillus sp. UFPEDA 485 by aqueous two-phase systems using PEG/sodium sulfate
title_full_unstemmed Partitioning of fibrinolytic protease from Bacillus sp. UFPEDA 485 by aqueous two-phase systems using PEG/sodium sulfate
title_sort Partitioning of fibrinolytic protease from Bacillus sp. UFPEDA 485 by aqueous two-phase systems using PEG/sodium sulfate
author Souza, F.A.S.D.
author_facet Souza, F.A.S.D.
Nascimento, J.C.S.
Sales, A. E.
Silva, Nuno Miguel Sampaio Ribeiro Magalhães
Marcos, J. C.
Porto, T. S
Teixeira, J. A.
Porto, A. L. F.
author_role author
author2 Nascimento, J.C.S.
Sales, A. E.
Silva, Nuno Miguel Sampaio Ribeiro Magalhães
Marcos, J. C.
Porto, T. S
Teixeira, J. A.
Porto, A. L. F.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Souza, F.A.S.D.
Nascimento, J.C.S.
Sales, A. E.
Silva, Nuno Miguel Sampaio Ribeiro Magalhães
Marcos, J. C.
Porto, T. S
Teixeira, J. A.
Porto, A. L. F.
dc.subject.por.fl_str_mv ATPS
PEG/Sodium Sulfate
Fibrinolytic protease
Bacillus
Ciências Médicas::Biotecnologia Médica
topic ATPS
PEG/Sodium Sulfate
Fibrinolytic protease
Bacillus
Ciências Médicas::Biotecnologia Médica
description XI Reunião Regional Nordeste da SBBq | 4th International Symposium in Biochemistry of Macromolecules and Biotechnology
publishDate 2012
dc.date.none.fl_str_mv 2012-12-05
2012-12-05T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/48386
url http://hdl.handle.net/1822/48386
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Souza, F.; Nascimento, J.; Sales, A. E.; Silva, N. M.; Marcos, J. C.; Porto, T. S; Teixeira, José A.; Porto, A. L. F., Partitioning of fibrinolytic protease from Bacillus sp. UFPEDA 485 by aqueous two-phase systems using PEG/sodium sulfate. XI Reunião Regional Nordeste da SBBq | 4th International Symposium in Biochemistry of Macromolecules and Biotechnology. Recife, Brazil, Dec 5-7, 2012.
http://www.sbbq.org.br/v2/index.php?option=com_content&task=view&id=746
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833594899851640832

Similar Items