Synergistically enhanced stability of laccase immobilized on synthesized silver nanoparticles with water-soluble polymers

Bibliographic Details
Main Author: Cunha, M. N. M.
Publication Date: 2017
Other Authors: Felgueiras, Helena Prado, Gouveia, Isabel, Zille, Andrea
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: https://hdl.handle.net/1822/45013
Summary: "In Press, Accepted Manuscript, Available online 12 March 2017"
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network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
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spelling Synergistically enhanced stability of laccase immobilized on synthesized silver nanoparticles with water-soluble polymersSilver nanoparticlesEnzymatic stabilityLaccaseSynergistic effectPolymer stabilizerEngenharia e Tecnologia::NanotecnologiaScience & Technology"In Press, Accepted Manuscript, Available online 12 March 2017"Silver nanoparticles (AgNPs) were synthesized by citrate reduction method in the presence of polymers, poly(ethylene glycol) (PEG), poly(vinyl alcohol) (PVA) and chitosan, used as stabilizing agents, and an oxidoreductase enzyme, laccase (Lac), with the goal of expanding the NPs antimicrobial action. AgNPs were characterized by UV-visible spectrometry, dynamic light scattering and transmission electron microscopy. As protecting agents, PEG and PVA promoted the formation of spherical uniformly-shaped, small-sized, monodispersed AgNPs (≈ 20 nm). High Mw polymers were established as most effective in producing small-sized NPs. Chitosan's viscosity led to the formation of aggregates. Despite the decrease in Lac activity registered for the hybrid formulation, AgNPs-polymer-Lac, a significant augment in stability over time (up to 13 days, at 50 °C) was observed. This novel formulation displays improved synergistic performance over AgNPs-Lac or polymer-Lac conjugates, since in the former the Lac activity becomes residual at the end of 3 days. By enabling many ionic interactions, chitosan restricted the mass transfer between Lac and substrate and, thus, inhibited the enzymatic activity. These hybrid nanocomposites made up of inorganic NPs, organic polymers and immobilized antimicrobial oxidoreductive enzymes represent a new class of materials with improved synergistic performance. Moreover, the Lac and the AgNPs different antimicrobial action, both in time and mechanism, may also constitute a new alternative to reduce the probability of developing resistance-associated mutations.This work was funded by Portuguese Foundation for Science and TechnologyFCT/MCTES (PIDDAC) and co-financed by European funds (FEDER) through the PT2020 program, research projectM-ERA-NET/0006/2014. A. Zille and H. P. Felgueiras also acknowledge funding from FCT within the scope of the project POCI-01-0145-FEDER-007136 and UID/CTM/00264.ElsevierUniversidade do MinhoCunha, M. N. M.Felgueiras, Helena PradoGouveia, IsabelZille, Andrea20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/45013eng0927-776510.1016/j.colsurfb.2017.03.02328343119info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-04-12T04:24:35Zoai:repositorium.sdum.uminho.pt:1822/45013Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:07:52.487685Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Synergistically enhanced stability of laccase immobilized on synthesized silver nanoparticles with water-soluble polymers
title Synergistically enhanced stability of laccase immobilized on synthesized silver nanoparticles with water-soluble polymers
spellingShingle Synergistically enhanced stability of laccase immobilized on synthesized silver nanoparticles with water-soluble polymers
Cunha, M. N. M.
Silver nanoparticles
Enzymatic stability
Laccase
Synergistic effect
Polymer stabilizer
Engenharia e Tecnologia::Nanotecnologia
Science & Technology
title_short Synergistically enhanced stability of laccase immobilized on synthesized silver nanoparticles with water-soluble polymers
title_full Synergistically enhanced stability of laccase immobilized on synthesized silver nanoparticles with water-soluble polymers
title_fullStr Synergistically enhanced stability of laccase immobilized on synthesized silver nanoparticles with water-soluble polymers
title_full_unstemmed Synergistically enhanced stability of laccase immobilized on synthesized silver nanoparticles with water-soluble polymers
title_sort Synergistically enhanced stability of laccase immobilized on synthesized silver nanoparticles with water-soluble polymers
author Cunha, M. N. M.
author_facet Cunha, M. N. M.
Felgueiras, Helena Prado
Gouveia, Isabel
Zille, Andrea
author_role author
author2 Felgueiras, Helena Prado
Gouveia, Isabel
Zille, Andrea
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Cunha, M. N. M.
Felgueiras, Helena Prado
Gouveia, Isabel
Zille, Andrea
dc.subject.por.fl_str_mv Silver nanoparticles
Enzymatic stability
Laccase
Synergistic effect
Polymer stabilizer
Engenharia e Tecnologia::Nanotecnologia
Science & Technology
topic Silver nanoparticles
Enzymatic stability
Laccase
Synergistic effect
Polymer stabilizer
Engenharia e Tecnologia::Nanotecnologia
Science & Technology
description "In Press, Accepted Manuscript, Available online 12 March 2017"
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/45013
url https://hdl.handle.net/1822/45013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0927-7765
10.1016/j.colsurfb.2017.03.023
28343119
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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