Microbial contamination in the coffee industry: an occupational menace besides a food safety concern?
| Main Author: | |
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| Publication Date: | 2022 |
| Other Authors: | , , , , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
| Download full: | http://hdl.handle.net/10400.5/95431 |
Summary: | © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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Microbial contamination in the coffee industry: an occupational menace besides a food safety concern?AspergillusOne Health approachAzole resistanceMilling stageMulti-approach for sampling and analyses© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).Respiratory abnormalities among workers at coffee roasting and packaging facilities have already been reported; however, little is known about microbiological contamination inside coffee production facilities. This study intends to assess the microbial contamination (fungi and bacteria) in two coffee industries from Brazil with a multi-approach protocol for sampling and for subsequent analyses using four main sources of samples: filtering respiratory protection devices (FRPD) used by workers, settled dust, electrostatic dust cloths (EDC) and coffee beans. The fungal contamination in the assessed industries was also characterized through the molecular detection of toxigenic species and antifungal resistance. Total bacteria contamination presented the highest values in FRPD collected from both industries (7.45 × 104 CFU·m-2; 1.09 × 104 CFU·m-2). Aspergillus genera was widespread in all the environmental samples collected and sections with clinical relevance (Fumigati) and with toxigenic potential (Nigri and Circumdati) were recovered from FRPD. Circumdati section was observed in 4 mg/mL itraconazole. Sections Circumdati (EDC, coffee beans and settled dust) and Nidulantes (EDC, coffee beans and FRPD) were detected by qPCR. Some of the targeted Aspergillus sections that have been identified microscopically were not detected by qPCR and vice-versa. Overall, this study revealed that microbial contamination is a potential occupational risk in the milling stage and should be tackled when assessing exposure and performing risk assessment. In addition, a multi-sampling campaign should be the approach to follow when assessing microbial contamination and FRPD should be included in this campaign. Occupational exposure to mycotoxins should be considered due to high fungal diversity and contamination. A One Health approach should address these issues in order to prevent consumption of coffee crops and beans infected by fungi and, more specifically, to avoid widespread azole resistance.This research was funded by H&TRC—Health & Technology Research Center, ESTeSL—Escola Superior de Tecnologia e Saúde, Instituto Politécnico de Lisboa, NOVA National School of Public Health, Public Health Research Centre, Universidade Nova de Lisboa, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001 and by FCT—Fundação para a Ciência e a Tecnologia, I.P. (Portugal), within the scope of the PhD Grant UI/BD/151431/2021.MDPIRepositório da Universidade de LisboaViegas, CarlaGomes, BiancaOliveira, FilipeDias, MartaCervantes, RenataPena, PedroGomes, Anita Q.Caetano, Liliana AranhaCarolino, Elisabetede Andrade, Ednilton TavaresViegas, Susana2024-11-19T13:58:00Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/95431engInt J Environ Res Public Health. 2022 Oct 18;19(20):134881661-782710.3390/ijerph1920134881660-4601info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T15:17:13Zoai:repositorio.ulisboa.pt:10400.5/95431Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T03:39:05.260805Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse |
| dc.title.none.fl_str_mv |
Microbial contamination in the coffee industry: an occupational menace besides a food safety concern? |
| title |
Microbial contamination in the coffee industry: an occupational menace besides a food safety concern? |
| spellingShingle |
Microbial contamination in the coffee industry: an occupational menace besides a food safety concern? Viegas, Carla Aspergillus One Health approach Azole resistance Milling stage Multi-approach for sampling and analyses |
| title_short |
Microbial contamination in the coffee industry: an occupational menace besides a food safety concern? |
| title_full |
Microbial contamination in the coffee industry: an occupational menace besides a food safety concern? |
| title_fullStr |
Microbial contamination in the coffee industry: an occupational menace besides a food safety concern? |
| title_full_unstemmed |
Microbial contamination in the coffee industry: an occupational menace besides a food safety concern? |
| title_sort |
Microbial contamination in the coffee industry: an occupational menace besides a food safety concern? |
| author |
Viegas, Carla |
| author_facet |
Viegas, Carla Gomes, Bianca Oliveira, Filipe Dias, Marta Cervantes, Renata Pena, Pedro Gomes, Anita Q. Caetano, Liliana Aranha Carolino, Elisabete de Andrade, Ednilton Tavares Viegas, Susana |
| author_role |
author |
| author2 |
Gomes, Bianca Oliveira, Filipe Dias, Marta Cervantes, Renata Pena, Pedro Gomes, Anita Q. Caetano, Liliana Aranha Carolino, Elisabete de Andrade, Ednilton Tavares Viegas, Susana |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Viegas, Carla Gomes, Bianca Oliveira, Filipe Dias, Marta Cervantes, Renata Pena, Pedro Gomes, Anita Q. Caetano, Liliana Aranha Carolino, Elisabete de Andrade, Ednilton Tavares Viegas, Susana |
| dc.subject.por.fl_str_mv |
Aspergillus One Health approach Azole resistance Milling stage Multi-approach for sampling and analyses |
| topic |
Aspergillus One Health approach Azole resistance Milling stage Multi-approach for sampling and analyses |
| description |
© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 2022-01-01T00:00:00Z 2024-11-19T13:58:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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http://hdl.handle.net/10400.5/95431 |
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http://hdl.handle.net/10400.5/95431 |
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eng |
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eng |
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Int J Environ Res Public Health. 2022 Oct 18;19(20):13488 1661-7827 10.3390/ijerph192013488 1660-4601 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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MDPI |
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MDPI |
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