NMR of Paramagnetic Proteins

Bibliographic Details
Main Author: Querci, Leonardo
Publication Date: 2023
Other Authors: Trindade, Inês B., Invernici, Michele, Silva, José Malanho, Cantini, Francesca, Louro, Ricardo O., Piccioli, Mario
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/158543
Summary: Funding Information: This project was supported from the PRIN2020-FAITH (Post-doctoral Fellowship to JMS). This work was funded by National funds through FCT-Fundaҫão para a Ciência e a Tecnologia, I. P. (FCT), project MOSTMICRO-ITQB with refs UIDB/04612/2020 and UIDP/04612/2020, and LS4FUTURE Associated Laboratory (LA/P/0087/2020). This work benefited from access to CERM/CIRMMP, the Instruct-ERIC Italy centre and from COST Action CA21115, supported by COST (European Cooperation in Science and Technology). Publisher Copyright: © 2023 by the authors.
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spelling NMR of Paramagnetic Proteins13C Derived Paramagnetic Relaxation Enhancements Are an Additional Source of Structural Information in SolutionHIPIPiron-sulfur proteinsmetalloproteinsNMR solution structureparamagnetic NMRparamagnetic relaxation enhancementstructural biologyElectronic, Optical and Magnetic MaterialsChemistry (miscellaneous)Materials ChemistryFunding Information: This project was supported from the PRIN2020-FAITH (Post-doctoral Fellowship to JMS). This work was funded by National funds through FCT-Fundaҫão para a Ciência e a Tecnologia, I. P. (FCT), project MOSTMICRO-ITQB with refs UIDB/04612/2020 and UIDP/04612/2020, and LS4FUTURE Associated Laboratory (LA/P/0087/2020). This work benefited from access to CERM/CIRMMP, the Instruct-ERIC Italy centre and from COST Action CA21115, supported by COST (European Cooperation in Science and Technology). Publisher Copyright: © 2023 by the authors.In paramagnetic metalloproteins, longitudinal relaxation rates of 13C′ and 13Cα nuclei can be measured using 13C detected experiments and converted into electron spin-nuclear spin distance restraints, also known as Paramagnetic Relaxation Enhancement (PRE) restraints. 13C are less sensitive to paramagnetism than 1H nuclei, therefore, 13C based PREs constitute an additional, non-redundant, structural information. We will discuss the complementarity of 13C PRE restraints with 1H PRE restraints in the case of the High Potential Iron Sulfur Protein (HiPIP) PioC, for which the NMR structure of PioC has been already solved by a combination of classical and paramagnetism-based restraints. We will show here that 13C R1 values can be measured also at very short distances from the paramagnetic center and that the obtained set of 13C based restraints can be added to 1H PREs and to other classical and paramagnetism based NMR restraints to improve quality and quantity of the NMR information.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNQuerci, LeonardoTrindade, Inês B.Invernici, MicheleSilva, José MalanhoCantini, FrancescaLouro, Ricardo O.Piccioli, Mario2023-09-30T22:21:31Z2023-02-262023-02-26T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/158543eng2312-7481PURE: 72625026https://doi.org/10.3390/magnetochemistry9030066info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T18:14:51Zoai:run.unl.pt:10362/158543Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:45:21.635135Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv NMR of Paramagnetic Proteins
13C Derived Paramagnetic Relaxation Enhancements Are an Additional Source of Structural Information in Solution
title NMR of Paramagnetic Proteins
spellingShingle NMR of Paramagnetic Proteins
Querci, Leonardo
HIPIP
iron-sulfur proteins
metalloproteins
NMR solution structure
paramagnetic NMR
paramagnetic relaxation enhancement
structural biology
Electronic, Optical and Magnetic Materials
Chemistry (miscellaneous)
Materials Chemistry
title_short NMR of Paramagnetic Proteins
title_full NMR of Paramagnetic Proteins
title_fullStr NMR of Paramagnetic Proteins
title_full_unstemmed NMR of Paramagnetic Proteins
title_sort NMR of Paramagnetic Proteins
author Querci, Leonardo
author_facet Querci, Leonardo
Trindade, Inês B.
Invernici, Michele
Silva, José Malanho
Cantini, Francesca
Louro, Ricardo O.
Piccioli, Mario
author_role author
author2 Trindade, Inês B.
Invernici, Michele
Silva, José Malanho
Cantini, Francesca
Louro, Ricardo O.
Piccioli, Mario
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Querci, Leonardo
Trindade, Inês B.
Invernici, Michele
Silva, José Malanho
Cantini, Francesca
Louro, Ricardo O.
Piccioli, Mario
dc.subject.por.fl_str_mv HIPIP
iron-sulfur proteins
metalloproteins
NMR solution structure
paramagnetic NMR
paramagnetic relaxation enhancement
structural biology
Electronic, Optical and Magnetic Materials
Chemistry (miscellaneous)
Materials Chemistry
topic HIPIP
iron-sulfur proteins
metalloproteins
NMR solution structure
paramagnetic NMR
paramagnetic relaxation enhancement
structural biology
Electronic, Optical and Magnetic Materials
Chemistry (miscellaneous)
Materials Chemistry
description Funding Information: This project was supported from the PRIN2020-FAITH (Post-doctoral Fellowship to JMS). This work was funded by National funds through FCT-Fundaҫão para a Ciência e a Tecnologia, I. P. (FCT), project MOSTMICRO-ITQB with refs UIDB/04612/2020 and UIDP/04612/2020, and LS4FUTURE Associated Laboratory (LA/P/0087/2020). This work benefited from access to CERM/CIRMMP, the Instruct-ERIC Italy centre and from COST Action CA21115, supported by COST (European Cooperation in Science and Technology). Publisher Copyright: © 2023 by the authors.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-30T22:21:31Z
2023-02-26
2023-02-26T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/158543
url http://hdl.handle.net/10362/158543
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2312-7481
PURE: 72625026
https://doi.org/10.3390/magnetochemistry9030066
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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