Using the MouseWalker to Quantify Locomotor Dysfunction in a Mouse Model of Spinal Cord Injury

Bibliographic Details
Main Author: Isidro, Ana Filipa
Publication Date: 2023
Other Authors: Medeiros, Alexandra M., Martins, Isaura, Neves-Silva, Dalila, Saúde, Leonor, Mendes, César S.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/168657
Summary: This work was supported by Fundação para a Ciência e a Tecnologia (FCT) (PTDC/BIA-COM/0151/2020), iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020), and LS4FUTURE (LA/P/0087/2020) to C.S.M. L.S. was supported by a CEEC Individual Principal Investigator contract (2021.02253.CEECIND). A.F.I. was supported by a doctoral fellowship from FCT (2020.08168.BD). A.M.M. was supported by a doctoral fellowship from FCT (PD/ BD/128445/2017). I.M. was supported by a post-doctoral fellowship from FCT (SFRH/BPD/118051/2016). D.N.S. was supported by a doctoral fellowship from FCT (SFRH/ BD/138636/2018).
id RCAP_bfa10f013d3cd0f7e126a01e8f007908
oai_identifier_str oai:run.unl.pt:10362/168657
network_acronym_str RCAP
network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository_id_str https://opendoar.ac.uk/repository/7160
spelling Using the MouseWalker to Quantify Locomotor Dysfunction in a Mouse Model of Spinal Cord InjuryNeuroscience(all)Chemical Engineering(all)Biochemistry, Genetics and Molecular Biology(all)Immunology and Microbiology(all)This work was supported by Fundação para a Ciência e a Tecnologia (FCT) (PTDC/BIA-COM/0151/2020), iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020), and LS4FUTURE (LA/P/0087/2020) to C.S.M. L.S. was supported by a CEEC Individual Principal Investigator contract (2021.02253.CEECIND). A.F.I. was supported by a doctoral fellowship from FCT (2020.08168.BD). A.M.M. was supported by a doctoral fellowship from FCT (PD/ BD/128445/2017). I.M. was supported by a post-doctoral fellowship from FCT (SFRH/BPD/118051/2016). D.N.S. was supported by a doctoral fellowship from FCT (SFRH/ BD/138636/2018).The execution of complex and highly coordinated motor programs, such as walking and running, is dependent on the rhythmic activation of spinal and supra-spinal circuits. After a thoracic spinal cord injury, communication with upstream circuits is impaired. This, in turn, leads to a loss of coordination, with limited recovery potential. Hence, to better evaluate the degree of recovery after the administration of drugs or therapies, there is a necessity for new, more detailed, and accurate tools to quantify gait, limb coordination, and other fine aspects of locomotor behavior in animal models of spinal cord injury. Several assays have been developed over the years to quantitatively assess free-walking behavior in rodents; however, they usually lack direct measurements related to stepping gait strategies, footprint patterns, and coordination. To address these shortcomings, an updated version of the MouseWalker, which combines a frustrated total internal reflection (fTIR) walkway with tracking and quantification software, is provided. This open-source system has been adapted to extract several graphical outputs and kinematic parameters, and a set of post-quantification tools can be to analyze the output data provided. This manuscript also demonstrates how this method, allied with already established behavioral tests, quantitatively describes locomotor deficits following spinal cord injury.iNOVA4Health - pólo NMSNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNIsidro, Ana FilipaMedeiros, Alexandra M.Martins, IsauraNeves-Silva, DalilaSaúde, LeonorMendes, César S.2025-03-25T01:31:37Z2023-03-242023-03-24T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/168657eng1940-087XPURE: 58482741https://doi.org/10.3791/65207info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-31T01:52:32Zoai:run.unl.pt:10362/168657Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:56:16.034547Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Using the MouseWalker to Quantify Locomotor Dysfunction in a Mouse Model of Spinal Cord Injury
title Using the MouseWalker to Quantify Locomotor Dysfunction in a Mouse Model of Spinal Cord Injury
spellingShingle Using the MouseWalker to Quantify Locomotor Dysfunction in a Mouse Model of Spinal Cord Injury
Isidro, Ana Filipa
Neuroscience(all)
Chemical Engineering(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)
title_short Using the MouseWalker to Quantify Locomotor Dysfunction in a Mouse Model of Spinal Cord Injury
title_full Using the MouseWalker to Quantify Locomotor Dysfunction in a Mouse Model of Spinal Cord Injury
title_fullStr Using the MouseWalker to Quantify Locomotor Dysfunction in a Mouse Model of Spinal Cord Injury
title_full_unstemmed Using the MouseWalker to Quantify Locomotor Dysfunction in a Mouse Model of Spinal Cord Injury
title_sort Using the MouseWalker to Quantify Locomotor Dysfunction in a Mouse Model of Spinal Cord Injury
author Isidro, Ana Filipa
author_facet Isidro, Ana Filipa
Medeiros, Alexandra M.
Martins, Isaura
Neves-Silva, Dalila
Saúde, Leonor
Mendes, César S.
author_role author
author2 Medeiros, Alexandra M.
Martins, Isaura
Neves-Silva, Dalila
Saúde, Leonor
Mendes, César S.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv iNOVA4Health - pólo NMS
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Isidro, Ana Filipa
Medeiros, Alexandra M.
Martins, Isaura
Neves-Silva, Dalila
Saúde, Leonor
Mendes, César S.
dc.subject.por.fl_str_mv Neuroscience(all)
Chemical Engineering(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)
topic Neuroscience(all)
Chemical Engineering(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)
description This work was supported by Fundação para a Ciência e a Tecnologia (FCT) (PTDC/BIA-COM/0151/2020), iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020), and LS4FUTURE (LA/P/0087/2020) to C.S.M. L.S. was supported by a CEEC Individual Principal Investigator contract (2021.02253.CEECIND). A.F.I. was supported by a doctoral fellowship from FCT (2020.08168.BD). A.M.M. was supported by a doctoral fellowship from FCT (PD/ BD/128445/2017). I.M. was supported by a post-doctoral fellowship from FCT (SFRH/BPD/118051/2016). D.N.S. was supported by a doctoral fellowship from FCT (SFRH/ BD/138636/2018).
publishDate 2023
dc.date.none.fl_str_mv 2023-03-24
2023-03-24T00:00:00Z
2025-03-25T01:31:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/168657
url http://hdl.handle.net/10362/168657
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1940-087X
PURE: 58482741
https://doi.org/10.3791/65207
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833597071018426368

Similar Items