Electrophoretic aqueous two-phase partition of proteins

Bibliographic Details
Main Author: Domingues, Lucília
Publication Date: 1996
Other Authors: Venâncio, Armando, Teixeira, J. A., Mota, M.
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/1822/33187
Summary: An electrical field was applied to an aqueous two-phase system. The integration of these techniques in one step is used to improve the partition of biological molecules. Experiments were performed for an individual protein - BSA - and for a mixture of proteins - lysozyme and egg albumin - at different polarities and opposite charges of the biomolecules for the aqueous two-phase system Reppal PES - PEG8000. The separation between those two proteins improved more than two-fold.
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spelling Electrophoretic aqueous two-phase partition of proteinsTwo-Phase Systems / Electrical partitionAn electrical field was applied to an aqueous two-phase system. The integration of these techniques in one step is used to improve the partition of biological molecules. Experiments were performed for an individual protein - BSA - and for a mixture of proteins - lysozyme and egg albumin - at different polarities and opposite charges of the biomolecules for the aqueous two-phase system Reppal PES - PEG8000. The separation between those two proteins improved more than two-fold.Universidade do MinhoDomingues, LucíliaVenâncio, ArmandoTeixeira, J. A.Mota, M.19961996-01-01T00:00:00Zconference paperinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/33187engDomingues, Lucília; Venâncio, Armando; Teixeira, J. A.; Mota, M., Electrophoretic aqueous two-phase partition of proteins. BIOTEC '96 - Comunicaciones del III Congreso Ibérico de Biotecnología. Valladolid, Spain, Sep., 81-84, 1996. ISBN: 84-7762-649-984-7762-649-9info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T07:15:51Zoai:repositorium.sdum.uminho.pt:1822/33187Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:20:59.422112Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Electrophoretic aqueous two-phase partition of proteins
title Electrophoretic aqueous two-phase partition of proteins
spellingShingle Electrophoretic aqueous two-phase partition of proteins
Domingues, Lucília
Two-Phase Systems / Electrical partition
title_short Electrophoretic aqueous two-phase partition of proteins
title_full Electrophoretic aqueous two-phase partition of proteins
title_fullStr Electrophoretic aqueous two-phase partition of proteins
title_full_unstemmed Electrophoretic aqueous two-phase partition of proteins
title_sort Electrophoretic aqueous two-phase partition of proteins
author Domingues, Lucília
author_facet Domingues, Lucília
Venâncio, Armando
Teixeira, J. A.
Mota, M.
author_role author
author2 Venâncio, Armando
Teixeira, J. A.
Mota, M.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Domingues, Lucília
Venâncio, Armando
Teixeira, J. A.
Mota, M.
dc.subject.por.fl_str_mv Two-Phase Systems / Electrical partition
topic Two-Phase Systems / Electrical partition
description An electrical field was applied to an aqueous two-phase system. The integration of these techniques in one step is used to improve the partition of biological molecules. Experiments were performed for an individual protein - BSA - and for a mixture of proteins - lysozyme and egg albumin - at different polarities and opposite charges of the biomolecules for the aqueous two-phase system Reppal PES - PEG8000. The separation between those two proteins improved more than two-fold.
publishDate 1996
dc.date.none.fl_str_mv 1996
1996-01-01T00:00:00Z
dc.type.driver.fl_str_mv conference paper
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/33187
url http://hdl.handle.net/1822/33187
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Domingues, Lucília; Venâncio, Armando; Teixeira, J. A.; Mota, M., Electrophoretic aqueous two-phase partition of proteins. BIOTEC '96 - Comunicaciones del III Congreso Ibérico de Biotecnología. Valladolid, Spain, Sep., 81-84, 1996. ISBN: 84-7762-649-9
84-7762-649-9
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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