Monitoring biotechnological processes through quantitative image analysis: application to 2-phenylethanol production by Yarrowia lipolytica

Detalhes bibliográficos
Autor(a) principal: Braga, Adelaide Correia
Data de Publicação: 2023
Outros Autores: Mesquita, D. P., Cordeiro, Ana, Belo, Isabel, Ferreira, Eugénio C., Amaral, A. Luís
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: https://hdl.handle.net/1822/83639
Resumo: Available online 20 March 2023
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spelling Monitoring biotechnological processes through quantitative image analysis: application to 2-phenylethanol production by Yarrowia lipolytica2-phenylethanol monitoringQuantitative image analysis (QIA)Multilinear regression (MLR)Yarrowia lipolyticaYeast and cluster morphologyAvailable online 20 March 2023Quantitative image analysis (QIA) is a simple and automated tool for process monitoring that, when combined with chemometric techniques, enables the association of changes in microbiota morphology to various operational parameters. To that effect, principal component analysis, multilinear regression, and ordinary least squares methods were applied to the obtained dataset of the biotransformation conditions for Y. lipolytica through the monitor of yeast morphology, substrates (glycerol, L-phenylalanine - L-Phe) consumption and metabolites (2-phenylethanol 2-PE) production was developed. Glycerol and L-Phe were successfully monitored by the proposed approach, though with a lower monitoring ability for 2-PE, and mostly related to yeast and cluster size and proportion, yeasts contents and cluster morphology. The chemometric approach also allowed to identify significant morphological modifications related with the change in the stirring speed in the experiments at 600rpm, 600/400rpm (600rpm for 24h, and 400rpm until the end of the experiment) and in pH from 5.5 to 7.5. This work demonstrated, for the first time, that QIA combined with chemometric analysis can be considered a valuable tool to monitor biotechnological processes, namely the 2-PE production by Y. lipolytica, by analyzing yeast and cluster morphology.FCT -Fundação para a Ciência e a Tecnologia(LA/P/0029/2020)info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoBraga, Adelaide CorreiaMesquita, D. P.Cordeiro, AnaBelo, IsabelFerreira, Eugénio C.Amaral, A. Luís2023-062023-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/83639engBraga, Adelaide; Mesquita, Daniela P.; Cordeiro, Ana; Ferreira, Eugénio C.; Amaral, A. Luís, Monitoring biotechnological processes through quantitative image analysis: Application to 2-phenylethanol production by Yarrowia lipolytica. Process Biochemistry, 129, 221-229, 20231359-51131873-329810.1016/j.procbio.2023.03.019http://www.journals.elsevier.com/process-biochemistry/info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-04-12T04:55:49Zoai:repositorium.sdum.uminho.pt:1822/83639Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:47:33.034457Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Monitoring biotechnological processes through quantitative image analysis: application to 2-phenylethanol production by Yarrowia lipolytica
title Monitoring biotechnological processes through quantitative image analysis: application to 2-phenylethanol production by Yarrowia lipolytica
spellingShingle Monitoring biotechnological processes through quantitative image analysis: application to 2-phenylethanol production by Yarrowia lipolytica
Braga, Adelaide Correia
2-phenylethanol monitoring
Quantitative image analysis (QIA)
Multilinear regression (MLR)
Yarrowia lipolytica
Yeast and cluster morphology
title_short Monitoring biotechnological processes through quantitative image analysis: application to 2-phenylethanol production by Yarrowia lipolytica
title_full Monitoring biotechnological processes through quantitative image analysis: application to 2-phenylethanol production by Yarrowia lipolytica
title_fullStr Monitoring biotechnological processes through quantitative image analysis: application to 2-phenylethanol production by Yarrowia lipolytica
title_full_unstemmed Monitoring biotechnological processes through quantitative image analysis: application to 2-phenylethanol production by Yarrowia lipolytica
title_sort Monitoring biotechnological processes through quantitative image analysis: application to 2-phenylethanol production by Yarrowia lipolytica
author Braga, Adelaide Correia
author_facet Braga, Adelaide Correia
Mesquita, D. P.
Cordeiro, Ana
Belo, Isabel
Ferreira, Eugénio C.
Amaral, A. Luís
author_role author
author2 Mesquita, D. P.
Cordeiro, Ana
Belo, Isabel
Ferreira, Eugénio C.
Amaral, A. Luís
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Braga, Adelaide Correia
Mesquita, D. P.
Cordeiro, Ana
Belo, Isabel
Ferreira, Eugénio C.
Amaral, A. Luís
dc.subject.por.fl_str_mv 2-phenylethanol monitoring
Quantitative image analysis (QIA)
Multilinear regression (MLR)
Yarrowia lipolytica
Yeast and cluster morphology
topic 2-phenylethanol monitoring
Quantitative image analysis (QIA)
Multilinear regression (MLR)
Yarrowia lipolytica
Yeast and cluster morphology
description Available online 20 March 2023
publishDate 2023
dc.date.none.fl_str_mv 2023-06
2023-06-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/83639
url https://hdl.handle.net/1822/83639
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Braga, Adelaide; Mesquita, Daniela P.; Cordeiro, Ana; Ferreira, Eugénio C.; Amaral, A. Luís, Monitoring biotechnological processes through quantitative image analysis: Application to 2-phenylethanol production by Yarrowia lipolytica. Process Biochemistry, 129, 221-229, 2023
1359-5113
1873-3298
10.1016/j.procbio.2023.03.019
http://www.journals.elsevier.com/process-biochemistry/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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