Structural transitions in orb2 prion-like domain relevant for functional aggregation in memory consolidation

Bibliographic Details
Main Author: Oroz, Javier
Publication Date: 2020
Other Authors: Félix, Sara S., Cabrita, Eurico J., Laurents, Douglas V.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/117153
Summary: Grant BBM_TRA_0203 PD/BD/148028/2019 UIDB/04378/2020
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network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
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spelling Structural transitions in orb2 prion-like domain relevant for functional aggregation in memory consolidationBiochemistryMolecular BiologyCell BiologyGrant BBM_TRA_0203 PD/BD/148028/2019 UIDB/04378/2020The recent structural elucidation of ex vivo Drosophila Orb2 fibrils revealed a novel amyloid formed by interdigitated Gln and His residue side chains belonging to the prion-like domain. However, atomic-level details on the conformational transitions associated with memory consolidation remain unknown. Here, we have characterized the nascent conformation and dynamics of the prion-like domain (PLD) of Orb2A using a nonconventional liquid-state NMR spectroscopy strategy based on 13C detection to afford an essentially complete set of 13Ca, 13Cb, 1Ha, and backbone 13CO and 15N assignments. At pH 4, where His residues are protonated, the PLD is disordered and flexible, except for a partially populated a-helix spanning residues 55–60, and binds RNA oligos, but not divalent cations. At pH 7, in contrast, His residues are predominantly neutral, and the Q/H segments adopt minor populations of helical structure, show decreased mobility and start to self-associate. At pH 7, the His residues do not bind RNA or Ca21, but do bind Zn21, which promotes further association. These findings represent a remarkable case of structural plasticity, based on which an updated model for Orb2A functional amyloidogenesis is suggested.DQ - Departamento de QuímicaUCIBIO - Applied Molecular Biosciences UnitRUNOroz, JavierFélix, Sara S.Cabrita, Eurico J.Laurents, Douglas V.2021-05-05T23:27:26Z2020-12-252020-12-25T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12application/pdfhttp://hdl.handle.net/10362/117153eng0021-9258PURE: 28079093https://doi.org/10.1074/jbc.RA120.015211info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:53:00Zoai:run.unl.pt:10362/117153Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:24:03.445374Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Structural transitions in orb2 prion-like domain relevant for functional aggregation in memory consolidation
title Structural transitions in orb2 prion-like domain relevant for functional aggregation in memory consolidation
spellingShingle Structural transitions in orb2 prion-like domain relevant for functional aggregation in memory consolidation
Oroz, Javier
Biochemistry
Molecular Biology
Cell Biology
title_short Structural transitions in orb2 prion-like domain relevant for functional aggregation in memory consolidation
title_full Structural transitions in orb2 prion-like domain relevant for functional aggregation in memory consolidation
title_fullStr Structural transitions in orb2 prion-like domain relevant for functional aggregation in memory consolidation
title_full_unstemmed Structural transitions in orb2 prion-like domain relevant for functional aggregation in memory consolidation
title_sort Structural transitions in orb2 prion-like domain relevant for functional aggregation in memory consolidation
author Oroz, Javier
author_facet Oroz, Javier
Félix, Sara S.
Cabrita, Eurico J.
Laurents, Douglas V.
author_role author
author2 Félix, Sara S.
Cabrita, Eurico J.
Laurents, Douglas V.
author2_role author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
UCIBIO - Applied Molecular Biosciences Unit
RUN
dc.contributor.author.fl_str_mv Oroz, Javier
Félix, Sara S.
Cabrita, Eurico J.
Laurents, Douglas V.
dc.subject.por.fl_str_mv Biochemistry
Molecular Biology
Cell Biology
topic Biochemistry
Molecular Biology
Cell Biology
description Grant BBM_TRA_0203 PD/BD/148028/2019 UIDB/04378/2020
publishDate 2020
dc.date.none.fl_str_mv 2020-12-25
2020-12-25T00:00:00Z
2021-05-05T23:27:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/117153
url http://hdl.handle.net/10362/117153
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0021-9258
PURE: 28079093
https://doi.org/10.1074/jbc.RA120.015211
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12
application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833596667654307840

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