Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections

Detalhes bibliográficos
Autor(a) principal: Barros, J.
Data de Publicação: 2020
Outros Autores: Melo, Luís Daniel Rodrigues, Silva, R. A., Ferraz, M. P., Azeredo, Joana, Pinheiro, V., Colaço, B., Fernandes, M. H., Gomes, P. S., Monteiro, F. J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: https://hdl.handle.net/1822/63341
Resumo: Supplementary data to this article can be found online at: https://doi.org/10.1016/j.nano.2019.102145.
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spelling Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infectionsBacteriophagesAlginate-nanohydroxyapatiteBiocompatibilityAntimicrobial activityOsteogenic differentiationScience & TechnologySupplementary data to this article can be found online at: https://doi.org/10.1016/j.nano.2019.102145.An innovative delivery system based on bacteriophages-loaded alginate-nanohydroxyapatite hydrogel was developed as a multifunctional approach for local tissue regeneration and infection prevention and control. Bacteriophages were efficiently encapsulated, without jeopardizing phage viability and functionality, nor affecting hydrogel morphology and chemical composition. Bacteriophage delivery occurred by swelling-disintegration-degradation process of the alginate structure and was influenced by environmental pH. Good tissue response was observed following the implantation of bacteriophages-loaded hydrogels, sustaining their biosafety profile. Bacteriophages-loaded hydrogels did not affect osteoblastic cells' proliferation and morphology. A strong osteogenic and mineralization response was promoted through the implantation of hydrogels system with nanohydroxyapatite. Lastly, bacteriophages-loaded hydrogel showed excellent antimicrobial activity inhibiting the attachment and colonization of multidrug-resistant E. faecalis surrounding and within femoral tissues. This new local delivery approach could be a promising approach to prevent and control bacterial contamination during implantation and bone integration.Funding: This work was supported by FEDER – Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020 – Operational Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, by Portuguese funds through FCT/MCTES in the framework of the project “institute for Research and Innovation in Health Sciences (POCI-01-0145-FEDER-007274), by Project Biotherapies (NORTE-01-0145-FEDER-000012) and by Joana Barrosʼ PhD grant (SFRH/BD/102148/2014). This work was also supported by Portuguese funds through FCT/MCTES (UID/QUI/50006/2019).info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoBarros, J.Melo, Luís Daniel RodriguesSilva, R. A.Ferraz, M. P.Azeredo, JoanaPinheiro, V.Colaço, B.Fernandes, M. H.Gomes, P. S.Monteiro, F. J.2020-022020-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/63341engBarros, J.; Melo, Luís D. R.; Silva, R. A.; Ferraz, M. P.; Azeredo, Joana; Pinheiro, V.; Colaço, B.; Fernandes, M. H.; Gomes, P. S.; Monteiro, F. J., Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections. Nanomedicine-Nanotechnology Biology and Medicine, 24(102145), 20201549-96341549-964210.1016/j.nano.2019.10214531857183http://www.nanomedjournal.com/info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-04-12T04:21:36Zoai:repositorium.sdum.uminho.pt:1822/63341Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:04:07.326438Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections
title Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections
spellingShingle Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections
Barros, J.
Bacteriophages
Alginate-nanohydroxyapatite
Biocompatibility
Antimicrobial activity
Osteogenic differentiation
Science & Technology
title_short Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections
title_full Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections
title_fullStr Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections
title_full_unstemmed Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections
title_sort Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections
author Barros, J.
author_facet Barros, J.
Melo, Luís Daniel Rodrigues
Silva, R. A.
Ferraz, M. P.
Azeredo, Joana
Pinheiro, V.
Colaço, B.
Fernandes, M. H.
Gomes, P. S.
Monteiro, F. J.
author_role author
author2 Melo, Luís Daniel Rodrigues
Silva, R. A.
Ferraz, M. P.
Azeredo, Joana
Pinheiro, V.
Colaço, B.
Fernandes, M. H.
Gomes, P. S.
Monteiro, F. J.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Barros, J.
Melo, Luís Daniel Rodrigues
Silva, R. A.
Ferraz, M. P.
Azeredo, Joana
Pinheiro, V.
Colaço, B.
Fernandes, M. H.
Gomes, P. S.
Monteiro, F. J.
dc.subject.por.fl_str_mv Bacteriophages
Alginate-nanohydroxyapatite
Biocompatibility
Antimicrobial activity
Osteogenic differentiation
Science & Technology
topic Bacteriophages
Alginate-nanohydroxyapatite
Biocompatibility
Antimicrobial activity
Osteogenic differentiation
Science & Technology
description Supplementary data to this article can be found online at: https://doi.org/10.1016/j.nano.2019.102145.
publishDate 2020
dc.date.none.fl_str_mv 2020-02
2020-02-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/63341
url https://hdl.handle.net/1822/63341
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Barros, J.; Melo, Luís D. R.; Silva, R. A.; Ferraz, M. P.; Azeredo, Joana; Pinheiro, V.; Colaço, B.; Fernandes, M. H.; Gomes, P. S.; Monteiro, F. J., Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections. Nanomedicine-Nanotechnology Biology and Medicine, 24(102145), 2020
1549-9634
1549-9642
10.1016/j.nano.2019.102145
31857183
http://www.nanomedjournal.com/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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