Mediated catalysis of Paracoccus pantotrophus cytochrome c peroxidase by P. pantotrophus pseudoazurin: kinetics of intermolecular electron transfer

Bibliographic Details
Main Author: Sousa, P. M. Paes de
Publication Date: 2007
Other Authors: Pauleta, Sofia R., Gonçalves, M. L. Simões, Pettigrew, Graham W., Moura, Isabel, Moura, José J. G., Santos, Margarida M. Correia dos
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/8703
Summary: J Biol Inorg Chem (2007) 12:691–698 DOI 10.1007/s00775-007-0219-9
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spelling Mediated catalysis of Paracoccus pantotrophus cytochrome c peroxidase by P. pantotrophus pseudoazurin: kinetics of intermolecular electron transferPseudoazurinCytochrome c peroxidaseCatalysisVoltammetryIntermolecular electron transferJ Biol Inorg Chem (2007) 12:691–698 DOI 10.1007/s00775-007-0219-9This work reports the direct electrochemistry of Paracoccus pantotrophus pseudoazurin and the mediated catalysis of cytochrome c peroxidase from the same organism. The voltammetric behaviour was examined at a gold membrane electrode, and the studies were performed in the presence of calcium to enable the peroxidase activation. A formal reduction potential, E (0)', of 230 +/- 5 mV was determined for pseudoazurin at pH 7.0. Its voltammetric signal presented a pH dependence, defined by pK values of 6.5 and 10.5 in the oxidised state and 7.2 in the reduced state, and was constant up to 1 M NaCl. This small copper protein was shown to be competent as an electron donor to cytochrome c peroxidase and the kinetics of intermolecular electron transfer was analysed. A second-order rate constant of 1.4 +/- 0.2 x 10(5) M(-1) s(-1) was determined at 0 M NaCl. This parameter has a maximum at 0.3 M NaCl and is pH-independent between pH 5 and 9.SpringerRUNSousa, P. M. Paes dePauleta, Sofia R.Gonçalves, M. L. SimõesPettigrew, Graham W.Moura, IsabelMoura, José J. G.Santos, Margarida M. Correia dos2013-02-06T10:27:52Z20072007-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/8703eng0949-8257info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:12:25Zoai:run.unl.pt:10362/8703Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:43:17.723865Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Mediated catalysis of Paracoccus pantotrophus cytochrome c peroxidase by P. pantotrophus pseudoazurin: kinetics of intermolecular electron transfer
title Mediated catalysis of Paracoccus pantotrophus cytochrome c peroxidase by P. pantotrophus pseudoazurin: kinetics of intermolecular electron transfer
spellingShingle Mediated catalysis of Paracoccus pantotrophus cytochrome c peroxidase by P. pantotrophus pseudoazurin: kinetics of intermolecular electron transfer
Sousa, P. M. Paes de
Pseudoazurin
Cytochrome c peroxidase
Catalysis
Voltammetry
Intermolecular electron transfer
title_short Mediated catalysis of Paracoccus pantotrophus cytochrome c peroxidase by P. pantotrophus pseudoazurin: kinetics of intermolecular electron transfer
title_full Mediated catalysis of Paracoccus pantotrophus cytochrome c peroxidase by P. pantotrophus pseudoazurin: kinetics of intermolecular electron transfer
title_fullStr Mediated catalysis of Paracoccus pantotrophus cytochrome c peroxidase by P. pantotrophus pseudoazurin: kinetics of intermolecular electron transfer
title_full_unstemmed Mediated catalysis of Paracoccus pantotrophus cytochrome c peroxidase by P. pantotrophus pseudoazurin: kinetics of intermolecular electron transfer
title_sort Mediated catalysis of Paracoccus pantotrophus cytochrome c peroxidase by P. pantotrophus pseudoazurin: kinetics of intermolecular electron transfer
author Sousa, P. M. Paes de
author_facet Sousa, P. M. Paes de
Pauleta, Sofia R.
Gonçalves, M. L. Simões
Pettigrew, Graham W.
Moura, Isabel
Moura, José J. G.
Santos, Margarida M. Correia dos
author_role author
author2 Pauleta, Sofia R.
Gonçalves, M. L. Simões
Pettigrew, Graham W.
Moura, Isabel
Moura, José J. G.
Santos, Margarida M. Correia dos
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Sousa, P. M. Paes de
Pauleta, Sofia R.
Gonçalves, M. L. Simões
Pettigrew, Graham W.
Moura, Isabel
Moura, José J. G.
Santos, Margarida M. Correia dos
dc.subject.por.fl_str_mv Pseudoazurin
Cytochrome c peroxidase
Catalysis
Voltammetry
Intermolecular electron transfer
topic Pseudoazurin
Cytochrome c peroxidase
Catalysis
Voltammetry
Intermolecular electron transfer
description J Biol Inorg Chem (2007) 12:691–698 DOI 10.1007/s00775-007-0219-9
publishDate 2007
dc.date.none.fl_str_mv 2007
2007-01-01T00:00:00Z
2013-02-06T10:27:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/8703
url http://hdl.handle.net/10362/8703
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0949-8257
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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