Ischaemia alters the effects of cardiomyocyte-derived extracellular vesicles on macrophage activation
| Main Author: | |
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| Publication Date: | 2018 |
| Other Authors: | , , , , , , , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
| Download full: | https://doi.org/10.1111/jcmm.14014 |
Summary: | We thank Dr Nuno Alves (Cardiology Department, CHUC‐HG) who performed the collection of human blood samples and Doctor Francisco Caramelo (iCBR/FMUC) for helping with the statistical analysis. This work was supported by the European Regional Development Fund (ERDF) through the Operational Program for Competitiveness Factors (COMPETE) [under the projects PAC “NETDIAMOND” POCI‐01‐0145‐FEDER‐016385; HealthyAging2020 CENTRO‐01‐0145‐ FEDER‐000012‐N2323; POCI‐01‐0145‐FEDER‐007440, CENTRO‐01‐ 0145‐FEDER‐032179, CENTRO‐01‐0145‐FEDER‐032414 and FCT‐ UID/NEU/04539/2013 to CNC.IBILI]. TMM was supported by PD/ BD/106043/2015 and TRR by PD/BD/52294/2013 from Fundação para a Ciência e a Tecnologia (FCT). JS was supported by Horizon2020 ERC‐2016‐COG EVICARE (725229). |
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Ischaemia alters the effects of cardiomyocyte-derived extracellular vesicles on macrophage activationacute myocardial infarctioncardiomyocytesextracellular vesiclesintercellular communicationmacrophagesMolecular MedicineCell BiologySDG 3 - Good Health and Well-beingWe thank Dr Nuno Alves (Cardiology Department, CHUC‐HG) who performed the collection of human blood samples and Doctor Francisco Caramelo (iCBR/FMUC) for helping with the statistical analysis. This work was supported by the European Regional Development Fund (ERDF) through the Operational Program for Competitiveness Factors (COMPETE) [under the projects PAC “NETDIAMOND” POCI‐01‐0145‐FEDER‐016385; HealthyAging2020 CENTRO‐01‐0145‐ FEDER‐000012‐N2323; POCI‐01‐0145‐FEDER‐007440, CENTRO‐01‐ 0145‐FEDER‐032179, CENTRO‐01‐0145‐FEDER‐032414 and FCT‐ UID/NEU/04539/2013 to CNC.IBILI]. TMM was supported by PD/ BD/106043/2015 and TRR by PD/BD/52294/2013 from Fundação para a Ciência e a Tecnologia (FCT). JS was supported by Horizon2020 ERC‐2016‐COG EVICARE (725229).Myocardial ischaemia is associated with an exacerbated inflammatory response, as well as with a deregulation of intercellular communication systems. Macrophages have been implicated in the maintenance of heart homeostasis and in the progression and resolution of the ischaemic injury. Nevertheless, the mechanisms underlying the crosstalk between cardiomyocytes and macrophages remain largely underexplored. Extracellular vesicles (EVs) have emerged as key players of cell-cell communication in cardiac health and disease. Hence, the main objective of this study was to characterize the impact of cardiomyocyte-derived EVs upon macrophage activation. Results obtained demonstrate that EVs released by H9c2 cells induced a pro-inflammatory profile in macrophages, via p38MAPK activation and increased expression of iNOS, IL-1β and IL-6, being these effects less pronounced with ischaemic EVs. EVs derived from neonatal cardiomyocytes, maintained either in control or ischaemia, induced a similar pattern of p38MAPK activation, expression of iNOS, IL-1β, IL-6, IL-10 and TNFα. Importantly, adhesion of macrophages to fibronectin was enhanced by EVs released by cardiomyocytes under ischaemia, whereas phagocytic capacity and adhesion to cardiomyocytes were higher in macrophages incubated with control EVs. Additionally, serum-circulating EVs isolated from human controls or acute myocardial infarction patients induce macrophage activation. According to our model, in basal conditions, cardiomyocyte-derived EVs maintain a macrophage profile that ensure heart homeostasis, whereas during ischaemia, this crosstalk is affected, likely impacting healing and post-infarction remodelling.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNAlmeida Paiva, RafaelMartins-Marques, TaniaJesus, KatiaRibeiro-Rodrigues, TeresaZuzarte, MonicaSilva, AnaReis, Lilianada Silva, MariaPereira, PauloVader, PieterPetrus Gerardus Sluijter, JoostGonçalves, LinoCruz, Maria TeresaGirao, Henrique2018-12-19T23:19:36Z2019-022019-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1111/jcmm.14014eng1582-1838PURE: 10660151http://www.scopus.com/inward/record.url?scp=85057757934&partnerID=8YFLogxKhttps://doi.org/10.1111/jcmm.14014info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:36:09Zoai:run.unl.pt:10362/55164Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:07:09.544670Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse |
| dc.title.none.fl_str_mv |
Ischaemia alters the effects of cardiomyocyte-derived extracellular vesicles on macrophage activation |
| title |
Ischaemia alters the effects of cardiomyocyte-derived extracellular vesicles on macrophage activation |
| spellingShingle |
Ischaemia alters the effects of cardiomyocyte-derived extracellular vesicles on macrophage activation Almeida Paiva, Rafael acute myocardial infarction cardiomyocytes extracellular vesicles intercellular communication macrophages Molecular Medicine Cell Biology SDG 3 - Good Health and Well-being |
| title_short |
Ischaemia alters the effects of cardiomyocyte-derived extracellular vesicles on macrophage activation |
| title_full |
Ischaemia alters the effects of cardiomyocyte-derived extracellular vesicles on macrophage activation |
| title_fullStr |
Ischaemia alters the effects of cardiomyocyte-derived extracellular vesicles on macrophage activation |
| title_full_unstemmed |
Ischaemia alters the effects of cardiomyocyte-derived extracellular vesicles on macrophage activation |
| title_sort |
Ischaemia alters the effects of cardiomyocyte-derived extracellular vesicles on macrophage activation |
| author |
Almeida Paiva, Rafael |
| author_facet |
Almeida Paiva, Rafael Martins-Marques, Tania Jesus, Katia Ribeiro-Rodrigues, Teresa Zuzarte, Monica Silva, Ana Reis, Liliana da Silva, Maria Pereira, Paulo Vader, Pieter Petrus Gerardus Sluijter, Joost Gonçalves, Lino Cruz, Maria Teresa Girao, Henrique |
| author_role |
author |
| author2 |
Martins-Marques, Tania Jesus, Katia Ribeiro-Rodrigues, Teresa Zuzarte, Monica Silva, Ana Reis, Liliana da Silva, Maria Pereira, Paulo Vader, Pieter Petrus Gerardus Sluijter, Joost Gonçalves, Lino Cruz, Maria Teresa Girao, Henrique |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Centro de Estudos de Doenças Crónicas (CEDOC) NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) RUN |
| dc.contributor.author.fl_str_mv |
Almeida Paiva, Rafael Martins-Marques, Tania Jesus, Katia Ribeiro-Rodrigues, Teresa Zuzarte, Monica Silva, Ana Reis, Liliana da Silva, Maria Pereira, Paulo Vader, Pieter Petrus Gerardus Sluijter, Joost Gonçalves, Lino Cruz, Maria Teresa Girao, Henrique |
| dc.subject.por.fl_str_mv |
acute myocardial infarction cardiomyocytes extracellular vesicles intercellular communication macrophages Molecular Medicine Cell Biology SDG 3 - Good Health and Well-being |
| topic |
acute myocardial infarction cardiomyocytes extracellular vesicles intercellular communication macrophages Molecular Medicine Cell Biology SDG 3 - Good Health and Well-being |
| description |
We thank Dr Nuno Alves (Cardiology Department, CHUC‐HG) who performed the collection of human blood samples and Doctor Francisco Caramelo (iCBR/FMUC) for helping with the statistical analysis. This work was supported by the European Regional Development Fund (ERDF) through the Operational Program for Competitiveness Factors (COMPETE) [under the projects PAC “NETDIAMOND” POCI‐01‐0145‐FEDER‐016385; HealthyAging2020 CENTRO‐01‐0145‐ FEDER‐000012‐N2323; POCI‐01‐0145‐FEDER‐007440, CENTRO‐01‐ 0145‐FEDER‐032179, CENTRO‐01‐0145‐FEDER‐032414 and FCT‐ UID/NEU/04539/2013 to CNC.IBILI]. TMM was supported by PD/ BD/106043/2015 and TRR by PD/BD/52294/2013 from Fundação para a Ciência e a Tecnologia (FCT). JS was supported by Horizon2020 ERC‐2016‐COG EVICARE (725229). |
| publishDate |
2018 |
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2018-12-19T23:19:36Z 2019-02 2019-02-01T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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publishedVersion |
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https://doi.org/10.1111/jcmm.14014 |
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https://doi.org/10.1111/jcmm.14014 |
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eng |
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eng |
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1582-1838 PURE: 10660151 http://www.scopus.com/inward/record.url?scp=85057757934&partnerID=8YFLogxK https://doi.org/10.1111/jcmm.14014 |
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