Natural Multimerization Rules the Performance of Affinity-Based Physical Hydrogels for Stem Cell Encapsulation and Differentiation

Bibliographic Details
Main Author: Fernandes, Cláudia S. M.
Publication Date: 2020
Other Authors: Rodrigues, André L., Alves, Vitor D., Fernandes, Tiago G., Pina, Ana Sofia, Roque, Ana Cecília A.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/141852
Summary: FCT/MEC (PTDC/BII-BIO/28878/2017; UIDB/04378/2020; UID/QUI/50006/2019; UID/AGR/04129/2019; POCI-01-0145FEDER-007728; LISBOA-01-0145-FEDER-028878); PD/BD/105871/2014; PD/BD/135524/2018;
id RCAP_b7ce5641b9a6dad326e40e7b142d77d2
oai_identifier_str oai:run.unl.pt:10362/141852
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network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
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spelling Natural Multimerization Rules the Performance of Affinity-Based Physical Hydrogels for Stem Cell Encapsulation and DifferentiationBioengineeringBiomaterialsPolymers and PlasticsMaterials ChemistryFCT/MEC (PTDC/BII-BIO/28878/2017; UIDB/04378/2020; UID/QUI/50006/2019; UID/AGR/04129/2019; POCI-01-0145FEDER-007728; LISBOA-01-0145-FEDER-028878); PD/BD/105871/2014; PD/BD/135524/2018;Tissue engineering and stem cell research greatly benefit from cell encapsulation within hydrogels as it promotes cell expansion and differentiation. Affinity-triggered hydrogels, an appealing solution for mild cell encapsulation, rely on selective interactions between the ligand and target and also on the multivalent presentation of these two components. Although these hydrogels represent a versatile option to generate dynamic, tunable, and highly functional materials, the design of hydrogel properties based on affinity and multivalency remains challenging and unstudied. Here, the avidin-biotin affinity pair, with the highest reported affinity constant, is used to address this challenge. It is demonstrated that the binding between the affinity hydrogel components is influenced by the multivalent display selected. In addition, the natural multivalency of the interaction must be obeyed to yield robust multicomponent synthetic protein hydrogels. The hydrogel's resistance to erosion depends on the right stoichiometric match between the hydrogel components. The developed affinity-triggered hydrogels are biocompatible and support encapsulation of induced pluripotent stem cells and their successful differentiation into a neural cell line. This principle can be generalized to other affinity pairs using multimeric proteins, yielding biomaterials with controlled performance.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNFernandes, Cláudia S. M.Rodrigues, André L.Alves, Vitor D.Fernandes, Tiago G.Pina, Ana SofiaRoque, Ana Cecília A.2022-07-13T22:20:35Z2020-08-102020-08-10T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://hdl.handle.net/10362/141852eng1525-7797PURE: 19976147https://doi.org/10.1021/acs.biomac.0c00473info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T18:03:26Zoai:run.unl.pt:10362/141852Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:34:15.702308Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Natural Multimerization Rules the Performance of Affinity-Based Physical Hydrogels for Stem Cell Encapsulation and Differentiation
title Natural Multimerization Rules the Performance of Affinity-Based Physical Hydrogels for Stem Cell Encapsulation and Differentiation
spellingShingle Natural Multimerization Rules the Performance of Affinity-Based Physical Hydrogels for Stem Cell Encapsulation and Differentiation
Fernandes, Cláudia S. M.
Bioengineering
Biomaterials
Polymers and Plastics
Materials Chemistry
title_short Natural Multimerization Rules the Performance of Affinity-Based Physical Hydrogels for Stem Cell Encapsulation and Differentiation
title_full Natural Multimerization Rules the Performance of Affinity-Based Physical Hydrogels for Stem Cell Encapsulation and Differentiation
title_fullStr Natural Multimerization Rules the Performance of Affinity-Based Physical Hydrogels for Stem Cell Encapsulation and Differentiation
title_full_unstemmed Natural Multimerization Rules the Performance of Affinity-Based Physical Hydrogels for Stem Cell Encapsulation and Differentiation
title_sort Natural Multimerization Rules the Performance of Affinity-Based Physical Hydrogels for Stem Cell Encapsulation and Differentiation
author Fernandes, Cláudia S. M.
author_facet Fernandes, Cláudia S. M.
Rodrigues, André L.
Alves, Vitor D.
Fernandes, Tiago G.
Pina, Ana Sofia
Roque, Ana Cecília A.
author_role author
author2 Rodrigues, André L.
Alves, Vitor D.
Fernandes, Tiago G.
Pina, Ana Sofia
Roque, Ana Cecília A.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Fernandes, Cláudia S. M.
Rodrigues, André L.
Alves, Vitor D.
Fernandes, Tiago G.
Pina, Ana Sofia
Roque, Ana Cecília A.
dc.subject.por.fl_str_mv Bioengineering
Biomaterials
Polymers and Plastics
Materials Chemistry
topic Bioengineering
Biomaterials
Polymers and Plastics
Materials Chemistry
description FCT/MEC (PTDC/BII-BIO/28878/2017; UIDB/04378/2020; UID/QUI/50006/2019; UID/AGR/04129/2019; POCI-01-0145FEDER-007728; LISBOA-01-0145-FEDER-028878); PD/BD/105871/2014; PD/BD/135524/2018;
publishDate 2020
dc.date.none.fl_str_mv 2020-08-10
2020-08-10T00:00:00Z
2022-07-13T22:20:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/141852
url http://hdl.handle.net/10362/141852
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1525-7797
PURE: 19976147
https://doi.org/10.1021/acs.biomac.0c00473
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11
application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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