Influence of Aldehyde Dehydrogenase Inhibition on Stemness of Endometrial Cancer Stem Cells
| Main Author: | |
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| Publication Date: | 2024 |
| Other Authors: | , , , , , , , , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
| Download full: | http://hdl.handle.net/10362/168824 |
Summary: | Publisher Copyright: © 2024 by the authors. |
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Influence of Aldehyde Dehydrogenase Inhibition on Stemness of Endometrial Cancer Stem Cellsaldehyde dehydrogenaseALDH inhibitioncancer stem cellsendometrial neoplasmsN,N-diethylaminobenzaldehydeproteomicsstemnessOncologyCancer ResearchSDG 3 - Good Health and Well-beingPublisher Copyright: © 2024 by the authors.Endometrial cancer is one of the most common gynaecological malignancies. Although often diagnosed at an early stage, there is a subset of patients with recurrent and metastatic disease for whom current treatments are not effective. Cancer stem cells (CSCs) play a pivotal role in triggering tumorigenesis, disease progression, recurrence, and metastasis, as high aldehyde dehydrogenase (ALDH) activity is associated with invasiveness and chemotherapy resistance. Therefore, this study aimed to evaluate the effects of ALDH inhibition in endometrial CSCs. ECC-1 and RL95-2 cells were submitted to a sphere-forming protocol to obtain endometrial CSCs. ALDH inhibition was evaluated through ALDH activity and expression, sphere-forming capacity, self-renewal, projection area, and CD133, CD44, CD24, and P53 expression. A mass spectrometry-based proteomic study was performed to determine the proteomic profile of endometrial cancer cells upon N,N-diethylaminobenzaldehyde (DEAB). DEAB reduced ALDH activity and expression, along with a significant decrease in sphere-forming capacity and projection area, with increased CD133 expression. Additionally, DEAB modulated P53 expression. Endometrial cancer cells display a distinct proteomic profile upon DEAB, sharing 75 up-regulated and 30 down-regulated proteins. In conclusion, DEAB inhibits ALDH activity and expression, influencing endometrial CSC phenotype. Furthermore, ALDH18A1, SdhA, and UBAP2L should be explored as novel molecular targets for endometrial cancer.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)iNOVA4Health - pólo NMSRUNSerambeque, BeatrizMestre, CatarinaCorreia-Barros, GabrielaTeixo, RicardoMarto, Carlos MiguelGonçalves, Ana CristinaCaramelo, FranciscoSilva, IsabelPaiva, ArturBeck, Hans C.Carvalho, Ana SofiaBotelho, Maria FilomenaCarvalho, Maria JoãoMatthiesen, RuneLaranjo, Mafalda2024-06-20T00:39:05Z2024-062024-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/168824eng2072-6694PURE: 93540217https://doi.org/10.3390/cancers16112031info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-06-24T01:36:45Zoai:run.unl.pt:10362/168824Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:56:23.464122Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse |
| dc.title.none.fl_str_mv |
Influence of Aldehyde Dehydrogenase Inhibition on Stemness of Endometrial Cancer Stem Cells |
| title |
Influence of Aldehyde Dehydrogenase Inhibition on Stemness of Endometrial Cancer Stem Cells |
| spellingShingle |
Influence of Aldehyde Dehydrogenase Inhibition on Stemness of Endometrial Cancer Stem Cells Serambeque, Beatriz aldehyde dehydrogenase ALDH inhibition cancer stem cells endometrial neoplasms N,N-diethylaminobenzaldehyde proteomics stemness Oncology Cancer Research SDG 3 - Good Health and Well-being |
| title_short |
Influence of Aldehyde Dehydrogenase Inhibition on Stemness of Endometrial Cancer Stem Cells |
| title_full |
Influence of Aldehyde Dehydrogenase Inhibition on Stemness of Endometrial Cancer Stem Cells |
| title_fullStr |
Influence of Aldehyde Dehydrogenase Inhibition on Stemness of Endometrial Cancer Stem Cells |
| title_full_unstemmed |
Influence of Aldehyde Dehydrogenase Inhibition on Stemness of Endometrial Cancer Stem Cells |
| title_sort |
Influence of Aldehyde Dehydrogenase Inhibition on Stemness of Endometrial Cancer Stem Cells |
| author |
Serambeque, Beatriz |
| author_facet |
Serambeque, Beatriz Mestre, Catarina Correia-Barros, Gabriela Teixo, Ricardo Marto, Carlos Miguel Gonçalves, Ana Cristina Caramelo, Francisco Silva, Isabel Paiva, Artur Beck, Hans C. Carvalho, Ana Sofia Botelho, Maria Filomena Carvalho, Maria João Matthiesen, Rune Laranjo, Mafalda |
| author_role |
author |
| author2 |
Mestre, Catarina Correia-Barros, Gabriela Teixo, Ricardo Marto, Carlos Miguel Gonçalves, Ana Cristina Caramelo, Francisco Silva, Isabel Paiva, Artur Beck, Hans C. Carvalho, Ana Sofia Botelho, Maria Filomena Carvalho, Maria João Matthiesen, Rune Laranjo, Mafalda |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) iNOVA4Health - pólo NMS RUN |
| dc.contributor.author.fl_str_mv |
Serambeque, Beatriz Mestre, Catarina Correia-Barros, Gabriela Teixo, Ricardo Marto, Carlos Miguel Gonçalves, Ana Cristina Caramelo, Francisco Silva, Isabel Paiva, Artur Beck, Hans C. Carvalho, Ana Sofia Botelho, Maria Filomena Carvalho, Maria João Matthiesen, Rune Laranjo, Mafalda |
| dc.subject.por.fl_str_mv |
aldehyde dehydrogenase ALDH inhibition cancer stem cells endometrial neoplasms N,N-diethylaminobenzaldehyde proteomics stemness Oncology Cancer Research SDG 3 - Good Health and Well-being |
| topic |
aldehyde dehydrogenase ALDH inhibition cancer stem cells endometrial neoplasms N,N-diethylaminobenzaldehyde proteomics stemness Oncology Cancer Research SDG 3 - Good Health and Well-being |
| description |
Publisher Copyright: © 2024 by the authors. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024-06-20T00:39:05Z 2024-06 2024-06-01T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10362/168824 |
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http://hdl.handle.net/10362/168824 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
2072-6694 PURE: 93540217 https://doi.org/10.3390/cancers16112031 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia instacron:RCAAP |
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Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
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Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia |
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