Hydroxycinnamic acids and curcumin production in engineered Escherichia coli using heat shock promoters

Bibliographic Details
Main Author: Rodrigues, Joana L.
Publication Date: 2017
Other Authors: Couto, Márcia, Araújo, R., Kristala L. J. Prather, Kluskens, Leon, Rodrigues, L. R.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: https://hdl.handle.net/1822/47426
Summary: Supplementary data associated with this article can be found, inthe online version, at http://dx.doi.org/10.1016/j.bej.2017.05.015.
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spelling Hydroxycinnamic acids and curcumin production in engineered Escherichia coli using heat shock promotersheat shock promotersE. colibiosynthetic pathwaycaffeic acidp-coumaric acidcurcuminScience & TechnologySupplementary data associated with this article can be found, inthe online version, at http://dx.doi.org/10.1016/j.bej.2017.05.015.Hydroxycinnamic acids and curcumin are compounds with great therapeutic potential, including anticancer properties. In this study, p-coumaric acid, caffeic acid and curcumin were produced in Escherichia coli. Their production was induced by heat using the dnaK and ibpA heat shock promoters. The ribosome binding site (RBS) used was tested and further optimized for each gene to assure an efficient translation. p-Coumaric acid was successfully produced from tyrosine and caffeic acid was produced either from tyrosine or p-coumaric acid using tyrosine ammonia lyase (TAL) from Rhodotorula glutinis, 4-coumarate 3-hydroxylase (C3H) from Saccharothrix espanaensis or cytochrome P450 CYP199A2 from Rhodopseudomonas palustris. The highest p-coumaric acid production obtained was 2.5 mM; caffeic acid production reached 370 M. Regarding curcumin, 17 M was produced using 4-coumarate-CoA ligase (4CL1) from Arabidopsis thaliana, diketide-CoA synthase (DCS) and curcumin synthase 1 (CURS1) from Curcuma longa. Stronger RBSs and/or different induction conditions should be further evaluated to optimize those production levels. Herein it was demonstrated that the biosynthetic pathway of p-coumaric acid, caffeic acid and curcumin in E. coli can be triggered by using heat shock promoters, suggesting its potential for the development of new industrial bioprocesses or even new bacterial therapies.FundingThis study was supported by the Portuguese Foundationfor Science and Technology (FCT) under the scope of thestrategic funding of UID/BIO/04469/2013 unit and COMPETE2020 (POCI-01-0145-FEDER-006684) and under the scope ofthe Project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462). The authors also acknowledge financial support fromBioTecNorte operation (NORTE-01-0145-FEDER-000004) and theproject MultiBiorefinery (POCI-01-0145-FEDER-016403) funded by the European Regional Development Fund under the scope ofNorte2020 − Programa Operacional Regional do Norte and the Post-doctoral grant (UMINHO/BPD/37/2015) to J. L. Rodrigues funded byFCT.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoRodrigues, Joana L.Couto, MárciaAraújo, R.Kristala L. J. PratherKluskens, LeonRodrigues, L. R.2017-09-152017-09-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/47426engRodrigues, Joana L.; Couto, Márcia; Araújo, R.; Kristala L. J. Prather; Kluskens, Leon D.; Rodrigues, Lígia R., Hydroxycinnamic acids and curcumin production in engineered Escherichia coli using heat shock promoters. Biochemical Engineering Journal, 125, 41-49, 20171369-703X10.1016/j.bej.2017.05.015http://www.elsevier.com/locate/issn/1369703Xinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-04-12T04:19:54Zoai:repositorium.sdum.uminho.pt:1822/47426Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:02:41.095369Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Hydroxycinnamic acids and curcumin production in engineered Escherichia coli using heat shock promoters
title Hydroxycinnamic acids and curcumin production in engineered Escherichia coli using heat shock promoters
spellingShingle Hydroxycinnamic acids and curcumin production in engineered Escherichia coli using heat shock promoters
Rodrigues, Joana L.
heat shock promoters
E. coli
biosynthetic pathway
caffeic acid
p-coumaric acid
curcumin
Science & Technology
title_short Hydroxycinnamic acids and curcumin production in engineered Escherichia coli using heat shock promoters
title_full Hydroxycinnamic acids and curcumin production in engineered Escherichia coli using heat shock promoters
title_fullStr Hydroxycinnamic acids and curcumin production in engineered Escherichia coli using heat shock promoters
title_full_unstemmed Hydroxycinnamic acids and curcumin production in engineered Escherichia coli using heat shock promoters
title_sort Hydroxycinnamic acids and curcumin production in engineered Escherichia coli using heat shock promoters
author Rodrigues, Joana L.
author_facet Rodrigues, Joana L.
Couto, Márcia
Araújo, R.
Kristala L. J. Prather
Kluskens, Leon
Rodrigues, L. R.
author_role author
author2 Couto, Márcia
Araújo, R.
Kristala L. J. Prather
Kluskens, Leon
Rodrigues, L. R.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Rodrigues, Joana L.
Couto, Márcia
Araújo, R.
Kristala L. J. Prather
Kluskens, Leon
Rodrigues, L. R.
dc.subject.por.fl_str_mv heat shock promoters
E. coli
biosynthetic pathway
caffeic acid
p-coumaric acid
curcumin
Science & Technology
topic heat shock promoters
E. coli
biosynthetic pathway
caffeic acid
p-coumaric acid
curcumin
Science & Technology
description Supplementary data associated with this article can be found, inthe online version, at http://dx.doi.org/10.1016/j.bej.2017.05.015.
publishDate 2017
dc.date.none.fl_str_mv 2017-09-15
2017-09-15T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/47426
url https://hdl.handle.net/1822/47426
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Rodrigues, Joana L.; Couto, Márcia; Araújo, R.; Kristala L. J. Prather; Kluskens, Leon D.; Rodrigues, Lígia R., Hydroxycinnamic acids and curcumin production in engineered Escherichia coli using heat shock promoters. Biochemical Engineering Journal, 125, 41-49, 2017
1369-703X
10.1016/j.bej.2017.05.015
http://www.elsevier.com/locate/issn/1369703X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833595062062153728

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