Tronchuda cabbage (Brassica oleracea L. var. costata DC) seeds: phytochemical characterization and antioxidant potential

• Main Author: Ferreres, Frederico
• Publication Date: 2007
• Other Authors: Sousa, Carla, Valentão, Patrícia, Seabra, Rosa M., Pereira, J.A., Andrade, Paula B.
• Format: Article
• Language: eng
• Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
• Download full: http://hdl.handle.net/10198/752
• topic: Tronchuda cabbage seeds; Brassica oleracea L. var. costata DC; Phenolic compounds; Organic acids; Antioxidant capacity

Tronchuda cabbage (Brassica oleracea L. var. costata DC) seeds were studied for their chemical composition and antioxidant capacity. Thirteen phenolic compounds were characterized and quantified by reversed-phase HPLC-DAD-MS/MS-ESI and HPLC-DAD, respectively: two sinapoylgentiobiose isomers, three sinapoylglucose isomers, kaempferol-3-(sinapoyl)sophorotrioside-7-glucoside, sinapoylcholine, kaempferol-3,7-diglucoside-40-(sinapoyl)glucoside, three disinapoylgentiobiose isomers, 1,2,20-trisinapoylgentiobiose and 1,2-disinapoylglucose. Seven organic acids (aconitic, citric, ascorbic, malic, quinic, shikimic and fumaric acids) were also identified and quantified by HPLC-UV. The aqueous extract of tronchuda cabbage seeds was investigated for its capacity to act as a scavenger of DPPH- radical and reactive oxygen species (superoxide radical, hydroxyl radical and hypochlorous acid), exhibiting antioxidant capacity in a concentration-dependent manner against all radicals. These results may be attributed to the high content of hydroxycinnamic derivatives and ascorbic acid.