Gold nanoparticles for photothermal therapy – Influence of experimental conditions on the properties of resulting AuNPs
| Main Author: | |
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| Publication Date: | 2024 |
| Other Authors: | , , , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
| Download full: | http://hdl.handle.net/10362/179496 |
Summary: | SFRH/BD/05377/2021. Publisher Copyright: © 2024 |
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Gold nanoparticles for photothermal therapy – Influence of experimental conditions on the properties of resulting AuNPsCancerGold nanoflowersGold nanoparticlesHyperthermiaNovel therapiesPhotothermal agentsPhotothermal therapyPharmaceutical ScienceSDG 3 - Good Health and Well-beingSFRH/BD/05377/2021. Publisher Copyright: © 2024Gold nanoparticles (AuNPs)-mediated Photothermal Therapy (PTT) is a minimally-invasive therapeutic approach that uses AuNPs to convert light into heat, leading to the thermal ablation of tumors. Thus, the efficacy of this strategy strongly relies on the photothermal conversion potential of AuNPs. The ability to convert light into heat can be enhanced by tuning the physicochemical and optical properties of AuNPs. This can be achieved by changing the conditions of AuNP's synthesis, such as the order of addition of reagents. The present work entails to explore how varying the order of reagents addition modulates the properties of AuNPs, particularly enhancing the photothermal conversion potential of the resulting AuNPs and consequently, improving PTT efficacy. For this, eleven different AuNPs' nanoformulations were synthetized following different sequences of addition of reagents. These nanoformulations were characterized regarding their physicochemical properties namely size, surface charge, gold concentration, surface morphology and maximum absorbance wavelength. In addition, their thermal activation profiles were determined in vitro. Furthermore, the biocompatibility of different nanoformulations was also assessed. Three nanoformulations, with the most favorable photothermal activation profiles (AuNPs 2, 3 and 7), were then selected for preliminary in vitro safety and efficacy assays using a panel of cell lines. These three nanoformulations were deemed safe in vitro at the tested concentrations. At 250 μM of gold content, and after an incubation period of 4 h, followed by 5 min irradiation with a laser emitting at 808 nm (7.96 W/cm2), AuNPs 7 significantly reduced the cell viability of all cancer cell lines tested (MCF-7, HCT-116 and A375) by ≥ 45 %. However, such cytotoxic effect was not observed for the human keratinocyte cell line (HaCat), thus demonstrating its specificity towards cancer cells. Overall, the results herein presented reinforce that the order of reagents addition is highly important for achieving adequate AuNPs for PTT.Faculdade de Ciências e Tecnologia (FCT)DCM - Departamento de Ciência dos MateriaisCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)DQ - Departamento de QuímicaCQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE)RUNAmaral, Mariana NevesNunes, DanielaFortunato, ElviraMartins, RodrigoRodrigues, CarlaFaísca, PedroFerreira, Hugo AlexandreCoelho, João M.P.Gaspar, M. ManuelaReis, Catarina Pinto2025-02-20T21:20:00Z2024-112024-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/179496eng1773-2247PURE: 107298552https://doi.org/10.1016/j.jddst.2024.106215info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-02-24T01:51:56Zoai:run.unl.pt:10362/179496Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T20:40:21.772981Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse |
| dc.title.none.fl_str_mv |
Gold nanoparticles for photothermal therapy – Influence of experimental conditions on the properties of resulting AuNPs |
| title |
Gold nanoparticles for photothermal therapy – Influence of experimental conditions on the properties of resulting AuNPs |
| spellingShingle |
Gold nanoparticles for photothermal therapy – Influence of experimental conditions on the properties of resulting AuNPs Amaral, Mariana Neves Cancer Gold nanoflowers Gold nanoparticles Hyperthermia Novel therapies Photothermal agents Photothermal therapy Pharmaceutical Science SDG 3 - Good Health and Well-being |
| title_short |
Gold nanoparticles for photothermal therapy – Influence of experimental conditions on the properties of resulting AuNPs |
| title_full |
Gold nanoparticles for photothermal therapy – Influence of experimental conditions on the properties of resulting AuNPs |
| title_fullStr |
Gold nanoparticles for photothermal therapy – Influence of experimental conditions on the properties of resulting AuNPs |
| title_full_unstemmed |
Gold nanoparticles for photothermal therapy – Influence of experimental conditions on the properties of resulting AuNPs |
| title_sort |
Gold nanoparticles for photothermal therapy – Influence of experimental conditions on the properties of resulting AuNPs |
| author |
Amaral, Mariana Neves |
| author_facet |
Amaral, Mariana Neves Nunes, Daniela Fortunato, Elvira Martins, Rodrigo Rodrigues, Carla Faísca, Pedro Ferreira, Hugo Alexandre Coelho, João M.P. Gaspar, M. Manuela Reis, Catarina Pinto |
| author_role |
author |
| author2 |
Nunes, Daniela Fortunato, Elvira Martins, Rodrigo Rodrigues, Carla Faísca, Pedro Ferreira, Hugo Alexandre Coelho, João M.P. Gaspar, M. Manuela Reis, Catarina Pinto |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Faculdade de Ciências e Tecnologia (FCT) DCM - Departamento de Ciência dos Materiais CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N) DQ - Departamento de Química CQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE) RUN |
| dc.contributor.author.fl_str_mv |
Amaral, Mariana Neves Nunes, Daniela Fortunato, Elvira Martins, Rodrigo Rodrigues, Carla Faísca, Pedro Ferreira, Hugo Alexandre Coelho, João M.P. Gaspar, M. Manuela Reis, Catarina Pinto |
| dc.subject.por.fl_str_mv |
Cancer Gold nanoflowers Gold nanoparticles Hyperthermia Novel therapies Photothermal agents Photothermal therapy Pharmaceutical Science SDG 3 - Good Health and Well-being |
| topic |
Cancer Gold nanoflowers Gold nanoparticles Hyperthermia Novel therapies Photothermal agents Photothermal therapy Pharmaceutical Science SDG 3 - Good Health and Well-being |
| description |
SFRH/BD/05377/2021. Publisher Copyright: © 2024 |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024-11 2024-11-01T00:00:00Z 2025-02-20T21:20:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/179496 |
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http://hdl.handle.net/10362/179496 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1773-2247 PURE: 107298552 https://doi.org/10.1016/j.jddst.2024.106215 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia instacron:RCAAP |
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FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia |
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RCAAP |
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Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
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Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
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Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia |
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