Bringing up to date the toolkit for the catabolism of aromatic compounds in fungi

Bibliographic Details
Main Author: Martins, Tiago M.
Publication Date: 2024
Other Authors: Bento, Artur, Martins, Celso, Tomé, Ana S., Moreira, Carlos J.S., Silva Pereira, Cristina
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/172691
Summary: Funding Information: The authors are grateful to Maria C. Leitão (ITQB-NOVA Small Molecules Analysis) for the implementation and support in the chromatographic analyses. Mass spectrometry data were generated by the Mass Spectrometry Unit (UniMS), ITQB/iBET, Oeiras, Portugal. All members of the Silva Pereira lab are also thanked for useful discussions. Publisher Copyright: © 2023 The Authors. Microbial Biotechnology published by Applied Microbiology International and John Wiley & Sons Ltd.
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spelling Bringing up to date the toolkit for the catabolism of aromatic compounds in fungiThe unexpected 1,2,3,5-tetrahydroxybenzene central pathwayBiotechnologyBioengineeringBiochemistryApplied Microbiology and BiotechnologyFunding Information: The authors are grateful to Maria C. Leitão (ITQB-NOVA Small Molecules Analysis) for the implementation and support in the chromatographic analyses. Mass spectrometry data were generated by the Mass Spectrometry Unit (UniMS), ITQB/iBET, Oeiras, Portugal. All members of the Silva Pereira lab are also thanked for useful discussions. Publisher Copyright: © 2023 The Authors. Microbial Biotechnology published by Applied Microbiology International and John Wiley & Sons Ltd.Saprophytic fungi are able to catabolize many plant-derived aromatics, including, for example, gallate. The catabolism of gallate in fungi is assumed to depend on the five main central pathways, i.e., of the central intermediates' catechol, protocatechuate, hydroxyquinol, homogentisate and gentisate, but a definitive demonstration is lacking. To shed light on this process, we analysed the transcriptional reprogramming of the growth of Aspergillus terreus on gallate compared with acetate as the control condition. Surprisingly, the results revealed that the five main central pathways did not exhibit significant positive regulation. Instead, an in-depth analysis identified four highly expressed and upregulated genes that are part of a conserved gene cluster found in numerous species of fungi, though not in Aspergilli. The cluster comprises a monooxygenase gene and a fumarylacetoacetate hydrolase-like gene, which are recognized as key components of catabolic pathways responsible for aromatic compound degradation. The other two genes encode proteins with no reported enzymatic activities. Through functional analyses of gene deletion mutants in Aspergillus nidulans, the conserved short protein with no known domains could be linked to the conversion of the novel metabolite 5-hydroxydienelatone, whereas the DUF3500 gene likely encodes a ring-cleavage enzyme for 1,2,3,5-tetrahydroxybenzene. These significant findings establish the existence of a new 1,2,3,5-tetrahydroxybenzene central pathway for the catabolism of gallate and related compounds (e.g. 2,4,6-trihydroxybenzoate) in numerous fungi where this catabolic gene cluster was observed.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNMartins, Tiago M.Bento, ArturMartins, CelsoTomé, Ana S.Moreira, Carlos J.S.Silva Pereira, Cristina2024-09-30T22:25:32Z2024-012024-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/172691eng1751-7907PURE: 90704476https://doi.org/10.1111/1751-7915.14371info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-10-07T01:38:25Zoai:run.unl.pt:10362/172691Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T18:55:17.060558Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Bringing up to date the toolkit for the catabolism of aromatic compounds in fungi
The unexpected 1,2,3,5-tetrahydroxybenzene central pathway
title Bringing up to date the toolkit for the catabolism of aromatic compounds in fungi
spellingShingle Bringing up to date the toolkit for the catabolism of aromatic compounds in fungi
Martins, Tiago M.
Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology
title_short Bringing up to date the toolkit for the catabolism of aromatic compounds in fungi
title_full Bringing up to date the toolkit for the catabolism of aromatic compounds in fungi
title_fullStr Bringing up to date the toolkit for the catabolism of aromatic compounds in fungi
title_full_unstemmed Bringing up to date the toolkit for the catabolism of aromatic compounds in fungi
title_sort Bringing up to date the toolkit for the catabolism of aromatic compounds in fungi
author Martins, Tiago M.
author_facet Martins, Tiago M.
Bento, Artur
Martins, Celso
Tomé, Ana S.
Moreira, Carlos J.S.
Silva Pereira, Cristina
author_role author
author2 Bento, Artur
Martins, Celso
Tomé, Ana S.
Moreira, Carlos J.S.
Silva Pereira, Cristina
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Martins, Tiago M.
Bento, Artur
Martins, Celso
Tomé, Ana S.
Moreira, Carlos J.S.
Silva Pereira, Cristina
dc.subject.por.fl_str_mv Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology
topic Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology
description Funding Information: The authors are grateful to Maria C. Leitão (ITQB-NOVA Small Molecules Analysis) for the implementation and support in the chromatographic analyses. Mass spectrometry data were generated by the Mass Spectrometry Unit (UniMS), ITQB/iBET, Oeiras, Portugal. All members of the Silva Pereira lab are also thanked for useful discussions. Publisher Copyright: © 2023 The Authors. Microbial Biotechnology published by Applied Microbiology International and John Wiley & Sons Ltd.
publishDate 2024
dc.date.none.fl_str_mv 2024-09-30T22:25:32Z
2024-01
2024-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/172691
url http://hdl.handle.net/10362/172691
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1751-7907
PURE: 90704476
https://doi.org/10.1111/1751-7915.14371
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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