Low frequency of enterohemorrhagic, enteroinvasive and diffusely adherent Escherichia coli in children under 5 years in rural Mozambique

Bibliographic Details
Main Author: Garrine, Marcelino
Publication Date: 2020
Other Authors: Matambisso, Glória, Nobela, Nélio, Vubil, Delfino, Massora, Sérgio, Acácio, Sozinho, Nhampossa, Tacilta, Alonso, Pedro, Mandomando, Inácio
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/180751
Summary: Funding information: The GEMS was funded by the Bill & Melinda Gates Foundation and coordinated by the Center for Vaccine Development, University of Maryland School of Medicine under the grant (OPP1033572). CISM receives core funding from Spanish Agency for International Cooperation and Development (AECID - Agencia Espanola de Cooperacion Internacional para el Desarollo) and the Government of Mozambique. Marcelino Garrine has a scholarship from Calouste Gulbenkian Foundation "Fundacao Calouste Gulbenkian" (grant number: 145278). The funders had no role in the study design, collection, analysis and interpretation of data, and manuscript writing.
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spelling Low frequency of enterohemorrhagic, enteroinvasive and diffusely adherent Escherichia coli in children under 5 years in rural Mozambiquea case-control studyChildrenDiarrheaDiarrheagenic Escherichia coli pathotypesPCRInfectious DiseasesSDG 3 - Good Health and Well-beingFunding information: The GEMS was funded by the Bill & Melinda Gates Foundation and coordinated by the Center for Vaccine Development, University of Maryland School of Medicine under the grant (OPP1033572). CISM receives core funding from Spanish Agency for International Cooperation and Development (AECID - Agencia Espanola de Cooperacion Internacional para el Desarollo) and the Government of Mozambique. Marcelino Garrine has a scholarship from Calouste Gulbenkian Foundation "Fundacao Calouste Gulbenkian" (grant number: 145278). The funders had no role in the study design, collection, analysis and interpretation of data, and manuscript writing.Background: Diarrheagenic Escherichia coli (DEC) are among the leading pathogens associated with endemic diarrhea in low income countries. Yet, few epidemiological studies have focused the contribution of enterohemorrhagic E. coli (EHEC), enteroinvasive E. coli (EIEC) and diffusely adherent E. coli (DAEC). Methods: We assessed the contribution of EHEC, EIEC and DAEC isolated from stool samples from a case-control study conducted in children aged < 5 years in Southern Mozambique between December 2007 and November 2012. The isolates were screened by conventional PCR targeting stx1 and stx2 (EHEC), ial and ipaH (EIEC), and daaE (DAEC) genes. Results: We analyzed 297 samples from cases with less-severe diarrhea (LSD) matched to 297 controls, and 89 samples from cases with moderate-to-severe diarrhea (MSD) matched to 222 controls, collected between November 3, 2011 and November 2, 2012. DEC were more common among LSD cases (2.7%, [8/297] of cases vs. 1.3% [4/297] of controls; p = 0.243]) than in MSD cases (0%, [0/89] of cases vs. 0.4%, [1/222] of controls; p = 1.000). Detailed analysis revealed low frequency of EHEC, DAEC or EIEC and no association with diarrhea in all age strata. Although the low frequency, EIEC was predominant in LSD cases aged 24-59 months (4.1% for cases vs. 0% for controls), followed by DAEC in similar frequency for cases and controls in infants (1.9%) and lastly EHEC from one control. Analysis of a subset of samples from previous period (December 10, 2007 and October 31, 2011) showed high frequency of DEC in controls compared to MSD cases (16.2%, [25/154] vs. 11.9%, [14/118], p = 0.383, respectively). Among these, DAEC predominated, being detected in 7.7% of cases vs. 17.6% of controls aged 24-59 months, followed by EIEC in 7.7% of cases vs. 5.9% of controls for the same age category, although no association was observed. EHEC was detected in one sample from cases and two from controls. Conclusions: Our data suggests that although EHEC, DAEC and EIEC are less frequent in endemic diarrhea in rural Mozambique, attention should be given to their transmission dynamics (e.g. the role on sporadic or epidemic diarrhea) considering that the role of asymptomatic individuals as source of dissemination remains unknown.TB, HIV and opportunistic diseases and pathogens (THOP)Global Health and Tropical Medicine (GHTM)Instituto de Higiene e Medicina Tropical (IHMT)RUNGarrine, MarcelinoMatambisso, GlóriaNobela, NélioVubil, DelfinoMassora, SérgioAcácio, SozinhoNhampossa, TaciltaAlonso, PedroMandomando, Inácio2025-03-17T21:09:58Z2020-09-072020-09-07T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6application/pdfhttp://hdl.handle.net/10362/180751eng2374-4235PURE: 26688407https://doi.org/10.1186/s12879-020-05380-1info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-31T02:00:45Zoai:run.unl.pt:10362/180751Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T04:42:02.463585Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Low frequency of enterohemorrhagic, enteroinvasive and diffusely adherent Escherichia coli in children under 5 years in rural Mozambique
a case-control study
title Low frequency of enterohemorrhagic, enteroinvasive and diffusely adherent Escherichia coli in children under 5 years in rural Mozambique
spellingShingle Low frequency of enterohemorrhagic, enteroinvasive and diffusely adherent Escherichia coli in children under 5 years in rural Mozambique
Garrine, Marcelino
Children
Diarrhea
Diarrheagenic Escherichia coli pathotypes
PCR
Infectious Diseases
SDG 3 - Good Health and Well-being
title_short Low frequency of enterohemorrhagic, enteroinvasive and diffusely adherent Escherichia coli in children under 5 years in rural Mozambique
title_full Low frequency of enterohemorrhagic, enteroinvasive and diffusely adherent Escherichia coli in children under 5 years in rural Mozambique
title_fullStr Low frequency of enterohemorrhagic, enteroinvasive and diffusely adherent Escherichia coli in children under 5 years in rural Mozambique
title_full_unstemmed Low frequency of enterohemorrhagic, enteroinvasive and diffusely adherent Escherichia coli in children under 5 years in rural Mozambique
title_sort Low frequency of enterohemorrhagic, enteroinvasive and diffusely adherent Escherichia coli in children under 5 years in rural Mozambique
author Garrine, Marcelino
author_facet Garrine, Marcelino
Matambisso, Glória
Nobela, Nélio
Vubil, Delfino
Massora, Sérgio
Acácio, Sozinho
Nhampossa, Tacilta
Alonso, Pedro
Mandomando, Inácio
author_role author
author2 Matambisso, Glória
Nobela, Nélio
Vubil, Delfino
Massora, Sérgio
Acácio, Sozinho
Nhampossa, Tacilta
Alonso, Pedro
Mandomando, Inácio
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv TB, HIV and opportunistic diseases and pathogens (THOP)
Global Health and Tropical Medicine (GHTM)
Instituto de Higiene e Medicina Tropical (IHMT)
RUN
dc.contributor.author.fl_str_mv Garrine, Marcelino
Matambisso, Glória
Nobela, Nélio
Vubil, Delfino
Massora, Sérgio
Acácio, Sozinho
Nhampossa, Tacilta
Alonso, Pedro
Mandomando, Inácio
dc.subject.por.fl_str_mv Children
Diarrhea
Diarrheagenic Escherichia coli pathotypes
PCR
Infectious Diseases
SDG 3 - Good Health and Well-being
topic Children
Diarrhea
Diarrheagenic Escherichia coli pathotypes
PCR
Infectious Diseases
SDG 3 - Good Health and Well-being
description Funding information: The GEMS was funded by the Bill & Melinda Gates Foundation and coordinated by the Center for Vaccine Development, University of Maryland School of Medicine under the grant (OPP1033572). CISM receives core funding from Spanish Agency for International Cooperation and Development (AECID - Agencia Espanola de Cooperacion Internacional para el Desarollo) and the Government of Mozambique. Marcelino Garrine has a scholarship from Calouste Gulbenkian Foundation "Fundacao Calouste Gulbenkian" (grant number: 145278). The funders had no role in the study design, collection, analysis and interpretation of data, and manuscript writing.
publishDate 2020
dc.date.none.fl_str_mv 2020-09-07
2020-09-07T00:00:00Z
2025-03-17T21:09:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/180751
url http://hdl.handle.net/10362/180751
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2374-4235
PURE: 26688407
https://doi.org/10.1186/s12879-020-05380-1
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
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collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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