Methods to Study Centrosomes and Cilia in Drosophila

Bibliographic Details
Main Author: Jana, Swadhin Chandra
Publication Date: 2016
Other Authors: Mendonça, Susana, Werner, Sascha, Bettencourt-Dias, Monica
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10400.7/832
Summary: Centrioles and cilia are highly conserved eukaryotic organelles. Drosophila melanogaster is a powerful genetic and cell biology model organism, extensively used to discover underlying mechanisms of centrosome and cilia biogenesis and function. Defects in centrosomes and cilia reduce fertility and affect different sensory functions, such as proprioception, olfaction, and hearing. The fly possesses a large diversity of ciliary structures and assembly modes, such as motile, immotile, and intraflagellar transport (IFT)-independent or IFT-dependent assembly. Moreover, all the diverse ciliated cells harbor centrioles at the base of the cilia, called basal bodies, making the fly an attractive model to better understand the biology of this organelle. This chapter describes protocols to visualize centrosomes and cilia by fluorescence and electron microscopy.
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spelling Methods to Study Centrosomes and Cilia in DrosophilaDrosophilaCentrosomeBasal bodyCiliaIntraflagellar transportSensory neuronSpermCentrioles and cilia are highly conserved eukaryotic organelles. Drosophila melanogaster is a powerful genetic and cell biology model organism, extensively used to discover underlying mechanisms of centrosome and cilia biogenesis and function. Defects in centrosomes and cilia reduce fertility and affect different sensory functions, such as proprioception, olfaction, and hearing. The fly possesses a large diversity of ciliary structures and assembly modes, such as motile, immotile, and intraflagellar transport (IFT)-independent or IFT-dependent assembly. Moreover, all the diverse ciliated cells harbor centrioles at the base of the cilia, called basal bodies, making the fly an attractive model to better understand the biology of this organelle. This chapter describes protocols to visualize centrosomes and cilia by fluorescence and electron microscopy.Humana PressARCAJana, Swadhin ChandraMendonça, SusanaWerner, SaschaBettencourt-Dias, Monica2018-02-07T17:16:59Z2016-08-122016-08-12T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/832eng10.1007/978-1-4939-3789-9_14info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-11-21T14:21:09Zoai:arca.igc.gulbenkian.pt:10400.7/832Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T19:15:19.110553Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Methods to Study Centrosomes and Cilia in Drosophila
title Methods to Study Centrosomes and Cilia in Drosophila
spellingShingle Methods to Study Centrosomes and Cilia in Drosophila
Jana, Swadhin Chandra
Drosophila
Centrosome
Basal body
Cilia
Intraflagellar transport
Sensory neuron
Sperm
title_short Methods to Study Centrosomes and Cilia in Drosophila
title_full Methods to Study Centrosomes and Cilia in Drosophila
title_fullStr Methods to Study Centrosomes and Cilia in Drosophila
title_full_unstemmed Methods to Study Centrosomes and Cilia in Drosophila
title_sort Methods to Study Centrosomes and Cilia in Drosophila
author Jana, Swadhin Chandra
author_facet Jana, Swadhin Chandra
Mendonça, Susana
Werner, Sascha
Bettencourt-Dias, Monica
author_role author
author2 Mendonça, Susana
Werner, Sascha
Bettencourt-Dias, Monica
author2_role author
author
author
dc.contributor.none.fl_str_mv ARCA
dc.contributor.author.fl_str_mv Jana, Swadhin Chandra
Mendonça, Susana
Werner, Sascha
Bettencourt-Dias, Monica
dc.subject.por.fl_str_mv Drosophila
Centrosome
Basal body
Cilia
Intraflagellar transport
Sensory neuron
Sperm
topic Drosophila
Centrosome
Basal body
Cilia
Intraflagellar transport
Sensory neuron
Sperm
description Centrioles and cilia are highly conserved eukaryotic organelles. Drosophila melanogaster is a powerful genetic and cell biology model organism, extensively used to discover underlying mechanisms of centrosome and cilia biogenesis and function. Defects in centrosomes and cilia reduce fertility and affect different sensory functions, such as proprioception, olfaction, and hearing. The fly possesses a large diversity of ciliary structures and assembly modes, such as motile, immotile, and intraflagellar transport (IFT)-independent or IFT-dependent assembly. Moreover, all the diverse ciliated cells harbor centrioles at the base of the cilia, called basal bodies, making the fly an attractive model to better understand the biology of this organelle. This chapter describes protocols to visualize centrosomes and cilia by fluorescence and electron microscopy.
publishDate 2016
dc.date.none.fl_str_mv 2016-08-12
2016-08-12T00:00:00Z
2018-02-07T17:16:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.7/832
url http://hdl.handle.net/10400.7/832
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1007/978-1-4939-3789-9_14
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Humana Press
publisher.none.fl_str_mv Humana Press
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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