Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2004 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
| Texto Completo: | http://hdl.handle.net/10362/1656 |
Resumo: | Eur. J. Biochem. 271, 1329–1338 (2004) |
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Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductaseAldehyde oxidoreductaseDesulfovibrio gigasElectrochemistryEur. J. Biochem. 271, 1329–1338 (2004)This work reports on the direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase (DgAOR), a molybdenum enzyme of the xanthine oxidase family that contains three redox-active cofactors: two [2Fe-2S] centers and a molybdopterin cytosine dinucleotide cofactor. The voltammetric behavior of the enzyme was analyzed at gold and carbon (pyrolytic graphite and glassy carbon) electrodes. Two different strategies were used: one with the molecules confined to the electrode surface and a second with DgAOR in solution. In all of the cases studied, electron transfer took place, although different redox reactions were responsible for the voltammetric signal. From a thorough analysis of the voltammetric responses and the structural properties of the molecular surface of DgAOR, the redox reaction at the carbon electrodes could be assigned to the reduction of the more exposed iron cluster, [2Fe-2S] II, whereas reduction of the molybdopterin cofactor occurs at the gold electrode. Voltammetric results in the presence of aldehydes are also reported and discussed.Blackwell PublishingRUNMoura, IsabelSantos, Margarida M. Correia dosSousa, Patrícia M. P.Gonçalves, M. Lurdes S.Romão, M. JoãoMoura, José J. G.2008-09-23T13:21:33Z20042004-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/1656eng00142956info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:06:18Zoai:run.unl.pt:10362/1656Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:37:10.264529Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse |
| dc.title.none.fl_str_mv |
Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase |
| title |
Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase |
| spellingShingle |
Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase Moura, Isabel Aldehyde oxidoreductase Desulfovibrio gigas Electrochemistry |
| title_short |
Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase |
| title_full |
Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase |
| title_fullStr |
Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase |
| title_full_unstemmed |
Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase |
| title_sort |
Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase |
| author |
Moura, Isabel |
| author_facet |
Moura, Isabel Santos, Margarida M. Correia dos Sousa, Patrícia M. P. Gonçalves, M. Lurdes S. Romão, M. João Moura, José J. G. |
| author_role |
author |
| author2 |
Santos, Margarida M. Correia dos Sousa, Patrícia M. P. Gonçalves, M. Lurdes S. Romão, M. João Moura, José J. G. |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
RUN |
| dc.contributor.author.fl_str_mv |
Moura, Isabel Santos, Margarida M. Correia dos Sousa, Patrícia M. P. Gonçalves, M. Lurdes S. Romão, M. João Moura, José J. G. |
| dc.subject.por.fl_str_mv |
Aldehyde oxidoreductase Desulfovibrio gigas Electrochemistry |
| topic |
Aldehyde oxidoreductase Desulfovibrio gigas Electrochemistry |
| description |
Eur. J. Biochem. 271, 1329–1338 (2004) |
| publishDate |
2004 |
| dc.date.none.fl_str_mv |
2004 2004-01-01T00:00:00Z 2008-09-23T13:21:33Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10362/1656 |
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http://hdl.handle.net/10362/1656 |
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eng |
| language |
eng |
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00142956 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Blackwell Publishing |
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Blackwell Publishing |
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reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia instacron:RCAAP |
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RCAAP |
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RCAAP |
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Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
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Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
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Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia |
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info@rcaap.pt |
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