Characterization of the azo reductase activity in a novel ascomycete yeast strain

Bibliographic Details
Main Author: Ramalho, Patrícia Alexandra Costa Ferreira
Publication Date: 2002
Other Authors: Cardoso, M. Helena, Paulo, Artur Cavaco, Ramalho, Maria Teresa
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: https://hdl.handle.net/1822/2201
Summary: Poster apresentado no XIII Congresso Nacional de Bioquímica, Lisboa, 5-7 Dezembro 2002.
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spelling Characterization of the azo reductase activity in a novel ascomycete yeast strainAzo reductaseAscomycete yeastPoster apresentado no XIII Congresso Nacional de Bioquímica, Lisboa, 5-7 Dezembro 2002.An ascomycete yeast strain, UM41 , was isolated from a textile wastewater treatment facility, and selected on the basis of its azo dye decolourising activity. Growing yeast cultures in liquid media containing glucose and 0.2mM dye removed more than 80% of the colour in 15 h, and total decolourisation of the tested dyes occured in 24 h. Colour loss is due to a constitutive azoreductase activity, expressed in intact cells, but not detectable in supernatant samples or cell-free extracts. This activity suffers thermal inactivation at temperatures higher than 50ºC and its dependence on dye concentration is satisfactorily described by the Michaelis-Menten model. Specific decolourising activities of resting cells have a maximum during the late exponential growth phase and are enhanced by up to 3-fold by the redox mediator AQS. The effect of pH on the decolourisation rates is structure-dependent. UM41 growing on glucose-containing medium initially exhibits a predominantly fermentative metabolism and produces ethanol, while oxygen is quicky depleted from the culture medium. However the same batch of cells retained decolourising activity for up to five successive cycles of dye removal, without any further nutrient addition. The microorganism growth and dye decolourisation observed after glucose exhaustion probably occur at the expense of ethanol, suggesting that decoulorisation is also compatible with a respiratory metabolism.BIOEFTEX.Universidade do MinhoRamalho, Patrícia Alexandra Costa FerreiraCardoso, M. HelenaPaulo, Artur CavacoRamalho, Maria Teresa2002-122002-12-01T00:00:00Zconference posterinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/1822/2201enginfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-15T01:17:02Zoai:repositorium.sdum.uminho.pt:1822/2201Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:36:45.815217Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Characterization of the azo reductase activity in a novel ascomycete yeast strain
title Characterization of the azo reductase activity in a novel ascomycete yeast strain
spellingShingle Characterization of the azo reductase activity in a novel ascomycete yeast strain
Ramalho, Patrícia Alexandra Costa Ferreira
Azo reductase
Ascomycete yeast
title_short Characterization of the azo reductase activity in a novel ascomycete yeast strain
title_full Characterization of the azo reductase activity in a novel ascomycete yeast strain
title_fullStr Characterization of the azo reductase activity in a novel ascomycete yeast strain
title_full_unstemmed Characterization of the azo reductase activity in a novel ascomycete yeast strain
title_sort Characterization of the azo reductase activity in a novel ascomycete yeast strain
author Ramalho, Patrícia Alexandra Costa Ferreira
author_facet Ramalho, Patrícia Alexandra Costa Ferreira
Cardoso, M. Helena
Paulo, Artur Cavaco
Ramalho, Maria Teresa
author_role author
author2 Cardoso, M. Helena
Paulo, Artur Cavaco
Ramalho, Maria Teresa
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Ramalho, Patrícia Alexandra Costa Ferreira
Cardoso, M. Helena
Paulo, Artur Cavaco
Ramalho, Maria Teresa
dc.subject.por.fl_str_mv Azo reductase
Ascomycete yeast
topic Azo reductase
Ascomycete yeast
description Poster apresentado no XIII Congresso Nacional de Bioquímica, Lisboa, 5-7 Dezembro 2002.
publishDate 2002
dc.date.none.fl_str_mv 2002-12
2002-12-01T00:00:00Z
dc.type.driver.fl_str_mv conference poster
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/2201
url https://hdl.handle.net/1822/2201
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
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reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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