Upper urinary tract urothelial tumours targeted with biodegradable drug-eluting stents

Bibliographic Details
Main Author: Barros, Alexandre António Antunes
Publication Date: 2016
Other Authors: Browne, S., Oliveira, C., Duarte, Ana Rita C., Reis, R. L., Healy, K. E., Lima, E.
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: https://hdl.handle.net/1822/42502
Summary: Publicado em "European urology supplements". Vol. 15, nr. 6 (2016)
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spelling Upper urinary tract urothelial tumours targeted with biodegradable drug-eluting stentsAnti-cancerBiodegradableUreteral stentsPublicado em "European urology supplements". Vol. 15, nr. 6 (2016)INTRODUCTION & OBJECTIVES: Biodegradable ureteral stents developed have proven to be an interesting alternative to conventional stents. Additionally, they could be applied as exceptional drug eluting stents, specially for urothelial tumours of upper urinary tract. In this case the conventional method of drug administration is via drug instillation. This has several drawbacks, such as high concentration dosage, increased side effects, short residence time and poor bioavailability. To avoid these problems, biodegradable ureteral stents impregnated by supercritical fluid CO2 (SCF) with four anti-cancer drugs were produced and their performance evaluated. MATERIAL & METHODS: The preparation of biodegradable stents was tested with four different formulations. The suitable concentration of gelatin and alginate and crosslinking agent was optimizedand bismuth was added to confer radiopaque properties to the stent. The preliminary in vivo validation studies of the biodegradable stents was conducted in female domestic pigs at the University of Minho, Braga, after formal approval by the institution’s review board and in accordance with its internal ethical protocol for animal experiments. In which concerns drug eluting stents, paclitaxel, epirubicin, doxorubicin and gemcitabine were impregnated and the release kinetics in artificial urine solution (AUS) was followed for 9 days by UV spectroscopy in a microplate reader. The anti-tumoral effect of the developed stents in cancer cell line (T24) and HUVEC primary cells, used as control, was evaluated. RESULTS: The in vivo validation of this second-generation of ureteral stents performed was herein demonstrated. Biodegradable ureteral stents were placed in the ureters of a female pig, following the normal surgical procedure. The animals remained asymptomatic, with normal urine flow. The in vitro release study in AUS of the stents impregnated showed a higher release in the first 72h for the four anti-cancer drugs impregnated and after this time a plateau was achieved and the stent degraded after 9 days. The direct and indirect contact of the anti-cancer biodegradable stents with the T24 and HUVEC cell lines confirm the anti-tumour effect of the stents impregnated with the four anti-cancer drugs, reducing around 75% of the viability of the T24 cells after 72h and no killing effect in the HUVEC cells. CONCLUSIONS: The use of biodegradable ureteral stents in urology clinical practice not only reduces the stent-related symptoms but also open new treatment therapy’s, like in urothelial tumours of upper urinary tract. We have herein demonstrated its clinical validation an in vivo model. And we have also shown the killing efficacy of the anti-cancer drug eluting biodegradable stents in vitro for the T24 cancer cells, with no toxicity observed in the control, non-cancer cells.ElsevierUniversidade do MinhoBarros, Alexandre António AntunesBrowne, S.Oliveira, C.Duarte, Ana Rita C.Reis, R. L.Healy, K. E.Lima, E.2016-072016-07-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/1822/42502engBarros A. A., Browne S., Oliveira C., Duarte A. R. C., Reis R. L., Healy K. E., Lima E. Upper urinary tract urothelial tumours targeted with biodegradable drug-eluting stents, ESUT 2016, Vol. 15, Issue 6, pp. 181, doi:10.1039/C2SM26784E, 20161569-9056http://ac.els-cdn.com/S1569905616151413/1-s2.0-S1569905616151413-main.pdf?_tid=100928ea-4844-11e6-ae2c-00000aacb35f&acdnat=1468336964_095c3e9dbd5b7e8da50ca5a61a786606info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-04-12T04:52:58Zoai:repositorium.sdum.uminho.pt:1822/42502Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:45:28.227668Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Upper urinary tract urothelial tumours targeted with biodegradable drug-eluting stents
title Upper urinary tract urothelial tumours targeted with biodegradable drug-eluting stents
spellingShingle Upper urinary tract urothelial tumours targeted with biodegradable drug-eluting stents
Barros, Alexandre António Antunes
Anti-cancer
Biodegradable
Ureteral stents
title_short Upper urinary tract urothelial tumours targeted with biodegradable drug-eluting stents
title_full Upper urinary tract urothelial tumours targeted with biodegradable drug-eluting stents
title_fullStr Upper urinary tract urothelial tumours targeted with biodegradable drug-eluting stents
title_full_unstemmed Upper urinary tract urothelial tumours targeted with biodegradable drug-eluting stents
title_sort Upper urinary tract urothelial tumours targeted with biodegradable drug-eluting stents
author Barros, Alexandre António Antunes
author_facet Barros, Alexandre António Antunes
Browne, S.
Oliveira, C.
Duarte, Ana Rita C.
Reis, R. L.
Healy, K. E.
Lima, E.
author_role author
author2 Browne, S.
Oliveira, C.
Duarte, Ana Rita C.
Reis, R. L.
Healy, K. E.
Lima, E.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Barros, Alexandre António Antunes
Browne, S.
Oliveira, C.
Duarte, Ana Rita C.
Reis, R. L.
Healy, K. E.
Lima, E.
dc.subject.por.fl_str_mv Anti-cancer
Biodegradable
Ureteral stents
topic Anti-cancer
Biodegradable
Ureteral stents
description Publicado em "European urology supplements". Vol. 15, nr. 6 (2016)
publishDate 2016
dc.date.none.fl_str_mv 2016-07
2016-07-01T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/42502
url https://hdl.handle.net/1822/42502
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Barros A. A., Browne S., Oliveira C., Duarte A. R. C., Reis R. L., Healy K. E., Lima E. Upper urinary tract urothelial tumours targeted with biodegradable drug-eluting stents, ESUT 2016, Vol. 15, Issue 6, pp. 181, doi:10.1039/C2SM26784E, 2016
1569-9056
http://ac.els-cdn.com/S1569905616151413/1-s2.0-S1569905616151413-main.pdf?_tid=100928ea-4844-11e6-ae2c-00000aacb35f&acdnat=1468336964_095c3e9dbd5b7e8da50ca5a61a786606
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833595519427936256

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