Gold nanoprobes for the detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis complex

Bibliographic Details
Main Author: Pedrosa, Pedro Miguel Pinto Gonçalves Gouveia
Publication Date: 2013
Format: Master thesis
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/10652
Summary: Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina
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network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
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spelling Gold nanoprobes for the detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis complexNanodiagnosticsGold nanoparticlesTuberculosisMultiplex PCRDissertação para obtenção do Grau de Mestre em Genética Molecular e BiomedicinaTuberculosis is still one of the leading human infectious diseases, with 8.7 million new cases reported in 2011 alone. Also, the increasing rate of multidrug-resistant tuberculosis (MDR-TB) and its treatment difficulties pose a serious public health threat especially in developing countries. Resistance to isoniazid and rifampicin, first line antibiotics, is commonly associated with point mutations in katG, inhA and rpoB genes of Mycobacterium tuberculosis complex (MTBC). Therefore, the development of a cheap, fast and simple molecular method to assess susceptibility profiles would have a huge impact in the capacity of early diagnosis and treatment of MDR-TB patients. Gold nanoparticles functionalised with thiol-modified oligonucleotides (Au-nanoprobes) have shown the potential to provide a rapid and sensitive detection method for MTBC and single base mutations associated with antibiotic resistance, namely the characterisation of the three most relevant codons in rpoB gene associated to rifampicin resistance. In this work the Au-nanoprobe approach is extended towards the discrimination of specific mutations within inhA gene which is associated with resistance to isoniazid. Using a multiplex PCR reaction for rpoB and inhA genes, it was possible to assess both loci in parallel, and extend the potential of the Au-nanoprobe method to MDR-TB molecular characterisation with special application in the most frequent genotypes circulating in the Lisbon Health Region.Faculdade de Ciências e TecnologiaBaptista, PedroRUNPedrosa, Pedro Miguel Pinto Gonçalves Gouveia2013-10-30T11:14:45Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/10652enginfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:14:28Zoai:run.unl.pt:10362/10652Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:45:34.814485Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Gold nanoprobes for the detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis complex
title Gold nanoprobes for the detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis complex
spellingShingle Gold nanoprobes for the detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis complex
Pedrosa, Pedro Miguel Pinto Gonçalves Gouveia
Nanodiagnostics
Gold nanoparticles
Tuberculosis
Multiplex PCR
title_short Gold nanoprobes for the detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis complex
title_full Gold nanoprobes for the detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis complex
title_fullStr Gold nanoprobes for the detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis complex
title_full_unstemmed Gold nanoprobes for the detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis complex
title_sort Gold nanoprobes for the detection of mutations associated with antibiotic resistance in Mycobacterium tuberculosis complex
author Pedrosa, Pedro Miguel Pinto Gonçalves Gouveia
author_facet Pedrosa, Pedro Miguel Pinto Gonçalves Gouveia
author_role author
dc.contributor.none.fl_str_mv Baptista, Pedro
RUN
dc.contributor.author.fl_str_mv Pedrosa, Pedro Miguel Pinto Gonçalves Gouveia
dc.subject.por.fl_str_mv Nanodiagnostics
Gold nanoparticles
Tuberculosis
Multiplex PCR
topic Nanodiagnostics
Gold nanoparticles
Tuberculosis
Multiplex PCR
description Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina
publishDate 2013
dc.date.none.fl_str_mv 2013-10-30T11:14:45Z
2013
2013-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/10652
url http://hdl.handle.net/10362/10652
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833596167533887488

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