First identification of genotypes of enterocytozoon bieneusi (Microsporidia) among symptomatic and asymptomatic children in Mozambique
| Autor(a) principal: | |
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| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
| Texto Completo: | http://hdl.handle.net/10362/180748 |
Resumo: | 870-15 SC (JM). The funders did not play any role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
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First identification of genotypes of enterocytozoon bieneusi (Microsporidia) among symptomatic and asymptomatic children in MozambiquePublic Health, Environmental and Occupational HealthInfectious DiseasesSDG 3 - Good Health and Well-being870-15 SC (JM). The funders did not play any role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.Enterocytozoon bieneusi is a human pathogen with a broad range of animal hosts. Initially, E. bieneusi was considered an emerging opportunistic pathogen in immunocompromised, mainly HIV-infected patients, but it has been increasingly reported in apparently healthy individuals globally. As in other African countries, the molecular epidemiology of E. bieneusi in Mozambique remains completely unknown. Therefore, we undertook a study to investigate the occurrence and genetic diversity of E. bieneusi infections in children with gastrointestinal symptoms as well as in asymptomatic children in Mozambique. Individual stool specimens were collected from 1,247 children aged between 0 and 14 years-old living in urban and rural settings in Zambézia (n = 1,097) and Maputo (n = 150) provinces between 2016 and 2019. Samples were analysed for E. bieneusi by nested-PCR targeting the internal transcribed spacer (ITS) region of the rRNA gene. All positive amplicons were confirmed and genotyped. Penalised logistic regression (Firth) was used to evaluate risk associations. The overall prevalence of E. bieneusi in this children population was 0.7% (9/1,247). A 10-fold higher prevalence was found in Maputo (4.0%; 6/150) than in Zambézia (0.3%; 3/1,097). All E. bieneusi-positive samples were from children older than 1-year of age, and most (8/9) from asymptomatic children. Nucleotide sequence analysis of the ITS region revealed the presence of four genotypes, three previously reported (Peru11, n = 1; Type IV, n = 2, and S2, n = 2) and a novel genotype (named HhMzEb1, n = 4). Novel genotype HhMzEb1 was identified in both asymptomatic (75%, 3/4) and symptomatic (25%, 1/4) children from a rural area in Maputo province in southern Mozambique. Genotypes HhMzEb1, Peru11, S2, and Type IV belonged to the Group 1 that includes genotypes with low host specificity and the potential for zoonotic and cross-species transmission. Being infected by enteric protozoan parasites and no handwashing were identified as risk associations for E. bieneusi infection. This study reports the first investigation of E. bieneusi genotypes in Mozambique with the identification of three previously reported genotypes in humans as well as a novel genotype (HhMzEb1). Findings highlight the need to conduct additional research to elucidate the epidemiology of E. bieneusi in the country, especially in rural areas where poor hygiene condi-tions still prevail. Special attention should be paid to the identification of suitable animal and environmental reservoirs of this parasite and to the characterization of transmission pathways.TB, HIV and opportunistic diseases and pathogens (THOP)Global Health and Tropical Medicine (GHTM)Instituto de Higiene e Medicina Tropical (IHMT)RUNMuadica, Aly S.Messa, Augusto E.Dashti, AlejandroBalasegaram, SooriaSantin, MónicaManjate, FilomenaChirinda, PercinaGarrine, MarcelinoVubil, DelfinoAcácio, SozinhoKöster, Pamela C.Bailo, BegoñaNhampossa, TaciltaCalero-Bernal, RafaelMwenda, Jason M.Mandomando, InácioCarmenaid, David2025-03-17T21:09:50Z2020-06-302020-06-30T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article19application/pdfhttp://hdl.handle.net/10362/180748eng1935-2727PURE: 26685622https://doi.org/10.1371/journal.pntd.0008419info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-31T02:00:42Zoai:run.unl.pt:10362/180748Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T04:42:02.291114Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse |
| dc.title.none.fl_str_mv |
First identification of genotypes of enterocytozoon bieneusi (Microsporidia) among symptomatic and asymptomatic children in Mozambique |
| title |
First identification of genotypes of enterocytozoon bieneusi (Microsporidia) among symptomatic and asymptomatic children in Mozambique |
| spellingShingle |
First identification of genotypes of enterocytozoon bieneusi (Microsporidia) among symptomatic and asymptomatic children in Mozambique Muadica, Aly S. Public Health, Environmental and Occupational Health Infectious Diseases SDG 3 - Good Health and Well-being |
| title_short |
First identification of genotypes of enterocytozoon bieneusi (Microsporidia) among symptomatic and asymptomatic children in Mozambique |
| title_full |
First identification of genotypes of enterocytozoon bieneusi (Microsporidia) among symptomatic and asymptomatic children in Mozambique |
| title_fullStr |
First identification of genotypes of enterocytozoon bieneusi (Microsporidia) among symptomatic and asymptomatic children in Mozambique |
| title_full_unstemmed |
First identification of genotypes of enterocytozoon bieneusi (Microsporidia) among symptomatic and asymptomatic children in Mozambique |
| title_sort |
First identification of genotypes of enterocytozoon bieneusi (Microsporidia) among symptomatic and asymptomatic children in Mozambique |
| author |
Muadica, Aly S. |
| author_facet |
Muadica, Aly S. Messa, Augusto E. Dashti, Alejandro Balasegaram, Sooria Santin, Mónica Manjate, Filomena Chirinda, Percina Garrine, Marcelino Vubil, Delfino Acácio, Sozinho Köster, Pamela C. Bailo, Begoña Nhampossa, Tacilta Calero-Bernal, Rafael Mwenda, Jason M. Mandomando, Inácio Carmenaid, David |
| author_role |
author |
| author2 |
Messa, Augusto E. Dashti, Alejandro Balasegaram, Sooria Santin, Mónica Manjate, Filomena Chirinda, Percina Garrine, Marcelino Vubil, Delfino Acácio, Sozinho Köster, Pamela C. Bailo, Begoña Nhampossa, Tacilta Calero-Bernal, Rafael Mwenda, Jason M. Mandomando, Inácio Carmenaid, David |
| author2_role |
author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
TB, HIV and opportunistic diseases and pathogens (THOP) Global Health and Tropical Medicine (GHTM) Instituto de Higiene e Medicina Tropical (IHMT) RUN |
| dc.contributor.author.fl_str_mv |
Muadica, Aly S. Messa, Augusto E. Dashti, Alejandro Balasegaram, Sooria Santin, Mónica Manjate, Filomena Chirinda, Percina Garrine, Marcelino Vubil, Delfino Acácio, Sozinho Köster, Pamela C. Bailo, Begoña Nhampossa, Tacilta Calero-Bernal, Rafael Mwenda, Jason M. Mandomando, Inácio Carmenaid, David |
| dc.subject.por.fl_str_mv |
Public Health, Environmental and Occupational Health Infectious Diseases SDG 3 - Good Health and Well-being |
| topic |
Public Health, Environmental and Occupational Health Infectious Diseases SDG 3 - Good Health and Well-being |
| description |
870-15 SC (JM). The funders did not play any role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-06-30 2020-06-30T00:00:00Z 2025-03-17T21:09:50Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://hdl.handle.net/10362/180748 |
| url |
http://hdl.handle.net/10362/180748 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1935-2727 PURE: 26685622 https://doi.org/10.1371/journal.pntd.0008419 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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19 application/pdf |
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FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia |
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RCAAP |
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RCAAP |
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Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
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Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
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Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia |
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info@rcaap.pt |
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1833602123741265920 |