Computational tools for strain optimization by tuning the optimal level of gene expression

Bibliographic Details
Main Author: Gonçalves, Emanuel
Publication Date: 2012
Other Authors: Rocha, I., Rocha, Miguel
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/1822/23378
Summary: In this work, a plug-in for the OptFlux Metabolic Engineering platform is presented, implementing methods that allow the identification of sets of genes to over/under express, relatively to their wild type levels. The optimization methods used are Simulated Annealing and Evolutionary Algorithms, working with a novel representation and operators. This overcomes the limitations of previous approaches based solely on gene knockouts, bringing new avenues for Biotechnology, fostering the discovery of genetic manipulations able to increase the production of certain compounds using a host microbe. The plug-in is made freely available together with appropriate documentation.
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spelling Computational tools for strain optimization by tuning the optimal level of gene expressionScience & TechnologyIn this work, a plug-in for the OptFlux Metabolic Engineering platform is presented, implementing methods that allow the identification of sets of genes to over/under express, relatively to their wild type levels. The optimization methods used are Simulated Annealing and Evolutionary Algorithms, working with a novel representation and operators. This overcomes the limitations of previous approaches based solely on gene knockouts, bringing new avenues for Biotechnology, fostering the discovery of genetic manipulations able to increase the production of certain compounds using a host microbe. The plug-in is made freely available together with appropriate documentation.Support of FCT and Programa COMPETE (ref. PTDC/EIA-EIA/ 115176/2009).SpringerSpringer VerlagUniversidade do MinhoGonçalves, EmanuelRocha, I.Rocha, Miguel20122012-01-01T00:00:00Zconference paperinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/23378eng97836422883881860-07941615-387110.1007/978-3-642-28839-5_29info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T06:17:06Zoai:repositorium.sdum.uminho.pt:1822/23378Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:47:38.174406Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Computational tools for strain optimization by tuning the optimal level of gene expression
title Computational tools for strain optimization by tuning the optimal level of gene expression
spellingShingle Computational tools for strain optimization by tuning the optimal level of gene expression
Gonçalves, Emanuel
Science & Technology
title_short Computational tools for strain optimization by tuning the optimal level of gene expression
title_full Computational tools for strain optimization by tuning the optimal level of gene expression
title_fullStr Computational tools for strain optimization by tuning the optimal level of gene expression
title_full_unstemmed Computational tools for strain optimization by tuning the optimal level of gene expression
title_sort Computational tools for strain optimization by tuning the optimal level of gene expression
author Gonçalves, Emanuel
author_facet Gonçalves, Emanuel
Rocha, I.
Rocha, Miguel
author_role author
author2 Rocha, I.
Rocha, Miguel
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Gonçalves, Emanuel
Rocha, I.
Rocha, Miguel
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description In this work, a plug-in for the OptFlux Metabolic Engineering platform is presented, implementing methods that allow the identification of sets of genes to over/under express, relatively to their wild type levels. The optimization methods used are Simulated Annealing and Evolutionary Algorithms, working with a novel representation and operators. This overcomes the limitations of previous approaches based solely on gene knockouts, bringing new avenues for Biotechnology, fostering the discovery of genetic manipulations able to increase the production of certain compounds using a host microbe. The plug-in is made freely available together with appropriate documentation.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
dc.type.driver.fl_str_mv conference paper
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/23378
url http://hdl.handle.net/1822/23378
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 9783642288388
1860-0794
1615-3871
10.1007/978-3-642-28839-5_29
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
Springer Verlag
publisher.none.fl_str_mv Springer
Springer Verlag
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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