Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death

Bibliographic Details
Main Author: Nolasco, Sofia
Publication Date: 2005
Other Authors: Bellido, Javier, Gonçalves, João, Zabala, Juan Carlos, Soares, Helena
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10400.21/3045
Summary: Microtubules are polymers of alpha/beta-tubulin participating in essential cell functions. A multistep process involving distinct molecular chaperones and cofactors produces new tubulin heterodimers competent to polymerise. In vitro cofactor A (TBCA) interacts with beta-tubulin in a quasi-native state behaving as a molecular chaperone. We have used siRNA to silence TBCA expression in HeLa and MCF-7 mammalian cell lines. TBCA is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest. In MCF-7 cells, cell death was preceded by a change in cell shape resembling differentiation.
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spelling Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell deathActin cytoskeletonApoptosisCaspase 7Cell cycleGene silencingMicrotubule-associated proteinsMicrotubulesMolecular chaperonesPhenotypeRNA, Small interferingTubulinβ-Tubulin cofactor AMolecular chaperoneMicrotubules are polymers of alpha/beta-tubulin participating in essential cell functions. A multistep process involving distinct molecular chaperones and cofactors produces new tubulin heterodimers competent to polymerise. In vitro cofactor A (TBCA) interacts with beta-tubulin in a quasi-native state behaving as a molecular chaperone. We have used siRNA to silence TBCA expression in HeLa and MCF-7 mammalian cell lines. TBCA is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest. In MCF-7 cells, cell death was preceded by a change in cell shape resembling differentiation.ElsevierRCIPLNolasco, SofiaBellido, JavierGonçalves, JoãoZabala, Juan CarlosSoares, Helena2014-01-01T19:01:02Z2005-072005-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/3045eng1873-3468info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-02-12T07:36:28Zoai:repositorio.ipl.pt:10400.21/3045Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T19:50:39.941787Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death
title Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death
spellingShingle Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death
Nolasco, Sofia
Actin cytoskeleton
Apoptosis
Caspase 7
Cell cycle
Gene silencing
Microtubule-associated proteins
Microtubules
Molecular chaperones
Phenotype
RNA, Small interfering
Tubulin
β-Tubulin cofactor A
Molecular chaperone
title_short Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death
title_full Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death
title_fullStr Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death
title_full_unstemmed Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death
title_sort Tubulin cofactor A gene silencing in mammalian cells induces changes in microtubule cytoskeleton, cell cycle arrest and cell death
author Nolasco, Sofia
author_facet Nolasco, Sofia
Bellido, Javier
Gonçalves, João
Zabala, Juan Carlos
Soares, Helena
author_role author
author2 Bellido, Javier
Gonçalves, João
Zabala, Juan Carlos
Soares, Helena
author2_role author
author
author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Nolasco, Sofia
Bellido, Javier
Gonçalves, João
Zabala, Juan Carlos
Soares, Helena
dc.subject.por.fl_str_mv Actin cytoskeleton
Apoptosis
Caspase 7
Cell cycle
Gene silencing
Microtubule-associated proteins
Microtubules
Molecular chaperones
Phenotype
RNA, Small interfering
Tubulin
β-Tubulin cofactor A
Molecular chaperone
topic Actin cytoskeleton
Apoptosis
Caspase 7
Cell cycle
Gene silencing
Microtubule-associated proteins
Microtubules
Molecular chaperones
Phenotype
RNA, Small interfering
Tubulin
β-Tubulin cofactor A
Molecular chaperone
description Microtubules are polymers of alpha/beta-tubulin participating in essential cell functions. A multistep process involving distinct molecular chaperones and cofactors produces new tubulin heterodimers competent to polymerise. In vitro cofactor A (TBCA) interacts with beta-tubulin in a quasi-native state behaving as a molecular chaperone. We have used siRNA to silence TBCA expression in HeLa and MCF-7 mammalian cell lines. TBCA is essential for cell viability and its knockdown produces a decrease in the amount of soluble tubulin, modifications in microtubules and G1 cell cycle arrest. In MCF-7 cells, cell death was preceded by a change in cell shape resembling differentiation.
publishDate 2005
dc.date.none.fl_str_mv 2005-07
2005-07-01T00:00:00Z
2014-01-01T19:01:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/3045
url http://hdl.handle.net/10400.21/3045
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1873-3468
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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