Human ceruloplasmin and neurotransmitters: complex stabilization and crystallization

Bibliographic Details
Main Author: Moutinho, Daniela Mesquita
Publication Date: 2013
Format: Master thesis
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/10853
Summary: Dissertation to obtain a Master Degree in Molecular Genetics and Biomedicine at Faculty of Sciences and Technology,Universidade Nova de Lisboa
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network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
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spelling Human ceruloplasmin and neurotransmitters: complex stabilization and crystallizationCeruloplasminNeurotransmittersEpinephrineDopamineL-dopaSerotoninDissertation to obtain a Master Degree in Molecular Genetics and Biomedicine at Faculty of Sciences and Technology,Universidade Nova de LisboaHuman ceruloplasmin (hCp) is the molecular linker between the copper and iron metabolism and its importance in the homeostasis of human body has been implied in some neurological diseases. This plasma cuproenzyme has ferroxidase activity, oxidizing Fe2+ to Fe3+ and incorporating it into apotransferrin. hCp also has aminoxidase activity regulating the levels of amine stress hormones in the bloodstream and brain. Thus, it is thought to have an important role in neurodegenerative diseases such as Alzheimer’s or Parkinson’s. To know more about the role of cerulopalsmin on the oxidation of neurotransmitters and on brain homeostasis it is essential to know which protein residues are implied in the binding and stabilization of these neurotransmitters. The primary source of structural information for protein-ligand complexes is X-ray crystallography. This is the most successful method to determine macromolecular 3D structures but has some limitations as obtaining good diffracting protein crystals. In this study several attempts were made to achieve better hCp diffracting crystals and crystals of hcp in complex with dopamine, L-dopa, epinephrine or serotonin in order to further determine its tridimensional structure. To improve hCp stabilization and solubility, differential scanning fluorimetry and dynamic light scattering were used in a search for a better buffer for crystallization. For hCp crystallization the vapour-diffusion technique was used in combination with several other methods. Commercial crystallization screens, crystal seeding, additives, crosslinking were the several methods used to improve crystal diffraction. Co-crystallization of hCp with neurotransmitters was performed with no success. Soaking of hCp crystals with the neurotransmitters was performed in an attempt to get crystals of the hCpneurotransmitter complexes. All crystals were sent for analysis at European Synchrotron Radiation Facility (ESRF) and structural data will be further processed.Faculdade de Ciências e TecnologiaBento, IsabelSampaio, José PauloRUNMoutinho, Daniela Mesquita2013-12-11T17:44:59Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/10853enginfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:14:40Zoai:run.unl.pt:10362/10853Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:45:43.457005Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Human ceruloplasmin and neurotransmitters: complex stabilization and crystallization
title Human ceruloplasmin and neurotransmitters: complex stabilization and crystallization
spellingShingle Human ceruloplasmin and neurotransmitters: complex stabilization and crystallization
Moutinho, Daniela Mesquita
Ceruloplasmin
Neurotransmitters
Epinephrine
Dopamine
L-dopa
Serotonin
title_short Human ceruloplasmin and neurotransmitters: complex stabilization and crystallization
title_full Human ceruloplasmin and neurotransmitters: complex stabilization and crystallization
title_fullStr Human ceruloplasmin and neurotransmitters: complex stabilization and crystallization
title_full_unstemmed Human ceruloplasmin and neurotransmitters: complex stabilization and crystallization
title_sort Human ceruloplasmin and neurotransmitters: complex stabilization and crystallization
author Moutinho, Daniela Mesquita
author_facet Moutinho, Daniela Mesquita
author_role author
dc.contributor.none.fl_str_mv Bento, Isabel
Sampaio, José Paulo
RUN
dc.contributor.author.fl_str_mv Moutinho, Daniela Mesquita
dc.subject.por.fl_str_mv Ceruloplasmin
Neurotransmitters
Epinephrine
Dopamine
L-dopa
Serotonin
topic Ceruloplasmin
Neurotransmitters
Epinephrine
Dopamine
L-dopa
Serotonin
description Dissertation to obtain a Master Degree in Molecular Genetics and Biomedicine at Faculty of Sciences and Technology,Universidade Nova de Lisboa
publishDate 2013
dc.date.none.fl_str_mv 2013-12-11T17:44:59Z
2013
2013-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/10853
url http://hdl.handle.net/10362/10853
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833596170806493184

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