Immobilization of bioactive factor-loaded liposomes at the surface of electrospun nanofibers targeting tissue engineering strategies

Bibliographic Details
Main Author: Monteiro, Nelson
Publication Date: 2013
Other Authors: Martins, Albino, Pires, R. A., Faria, Susana, Fonseca, Nuno A., Moreira, João N., Reis, R. L., Neves, N. M.
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/1822/27115
Summary: Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013)
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spelling Immobilization of bioactive factor-loaded liposomes at the surface of electrospun nanofibers targeting tissue engineering strategiesDexamethasoneDrug Delivery SystemElectrospun nanofibersLiposomesStem Cells DifferentiationPublicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013)The ability to manipulate and control the surface properties is of crucial importance in the designing of scaffolds for Tissue Engineering (TE) and Regenerative Medicine. Electrospun nanofibers (NFM), due to their morphology and fibrous structure have received much attention as potential biomedical devices, TE scaffolds and drug delivery carriers. Liposomes, a nanoparticle release system made by physiological material (phospholipids), hold tremendous promise as release systems. Liposomes may be combined with scaffolds to maintain a sustained and local delivery of the loaded drugs. The main objective of the present study is to evaluate the efficacy of dexamethasone (Dex) loaded liposomes immobilized on the surface of polycaprolactone (PCL) electrospun nanofiber meshes (NFM) as release system, for the induction of the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs). The PCL NFM surfaces were activated using the UV-Ozone irradiation technique. Aminolysis was performed to insert amine groups onto the NFM surfaces. Afterwards, SH groups were inserted at the surface of the NFMs through the reaction of the aminated surfaces with 2-iminothiolane. Ellman’s reagent method was used to quantify the SH groups onto the NFM surfaces. Dex-loaded liposomes were covalently immobilized at the surface of chemically functionalized electrospun PCL NFMs. The in vitro release profile demonstrated a sustained release of Dex during 21 days, after an initial burst release of 12 h. Biological assays showed that Dex-loaded liposomes immobilized at the surface of electrospun PCL NFMs did not exhibit any cytotoxic effect, promoting the osteogenic differentiation of hBMSCs. We herein validate the concept of using liposomes immobilized at the surface of a nanostructured fibrous system to be used as an advanced cell carrier device with autonomous release of growth/differentiation factors relevant for tissue engineering and regenerative medicine strategiesJohn Wiley and SonsUniversidade do MinhoMonteiro, NelsonMartins, AlbinoPires, R. A.Faria, SusanaFonseca, Nuno A.Moreira, João N.Reis, R. L.Neves, N. M.2013-092013-09-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/27115enghttp://onlinelibrary.wiley.com/doi/10.1002/term.2013.7.issue-s1/issuetocinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T05:06:45Zoai:repositorium.sdum.uminho.pt:1822/27115Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:08:04.154483Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Immobilization of bioactive factor-loaded liposomes at the surface of electrospun nanofibers targeting tissue engineering strategies
title Immobilization of bioactive factor-loaded liposomes at the surface of electrospun nanofibers targeting tissue engineering strategies
spellingShingle Immobilization of bioactive factor-loaded liposomes at the surface of electrospun nanofibers targeting tissue engineering strategies
Monteiro, Nelson
Dexamethasone
Drug Delivery System
Electrospun nanofibers
Liposomes
Stem Cells Differentiation
title_short Immobilization of bioactive factor-loaded liposomes at the surface of electrospun nanofibers targeting tissue engineering strategies
title_full Immobilization of bioactive factor-loaded liposomes at the surface of electrospun nanofibers targeting tissue engineering strategies
title_fullStr Immobilization of bioactive factor-loaded liposomes at the surface of electrospun nanofibers targeting tissue engineering strategies
title_full_unstemmed Immobilization of bioactive factor-loaded liposomes at the surface of electrospun nanofibers targeting tissue engineering strategies
title_sort Immobilization of bioactive factor-loaded liposomes at the surface of electrospun nanofibers targeting tissue engineering strategies
author Monteiro, Nelson
author_facet Monteiro, Nelson
Martins, Albino
Pires, R. A.
Faria, Susana
Fonseca, Nuno A.
Moreira, João N.
Reis, R. L.
Neves, N. M.
author_role author
author2 Martins, Albino
Pires, R. A.
Faria, Susana
Fonseca, Nuno A.
Moreira, João N.
Reis, R. L.
Neves, N. M.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Monteiro, Nelson
Martins, Albino
Pires, R. A.
Faria, Susana
Fonseca, Nuno A.
Moreira, João N.
Reis, R. L.
Neves, N. M.
dc.subject.por.fl_str_mv Dexamethasone
Drug Delivery System
Electrospun nanofibers
Liposomes
Stem Cells Differentiation
topic Dexamethasone
Drug Delivery System
Electrospun nanofibers
Liposomes
Stem Cells Differentiation
description Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013)
publishDate 2013
dc.date.none.fl_str_mv 2013-09
2013-09-01T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/27115
url http://hdl.handle.net/1822/27115
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://onlinelibrary.wiley.com/doi/10.1002/term.2013.7.issue-s1/issuetoc
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley and Sons
publisher.none.fl_str_mv John Wiley and Sons
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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