Molecular Markers of Sulfadoxine-Pyrimethamine Resistance in Samples from Children with Uncomplicated Plasmodium falciparum at Three Sites in Angola in 2019

Bibliographic Details
Main Author: Rosillo, Stefano R.
Publication Date: 2023
Other Authors: Dimbu, Pedro Rafael, Cândido, Ana Luisa M., Oh, Je Hoon Michael, Ferreira, Carolina Miguel, Andrade, Benjamin Nieto, Labuda, Sarah, Horth, Roberta, Kelley, Julia, Morais, Joana F.M., Fortes, Filomeno, Martins, José Franco, Talundzic, Eldin, Pluciński, Mateusz M.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/155095
Summary: Funding Information: The study was funded by the U.S. President’s Malaria Initiative and the Advanced Molecular Detection program at CDC. Funding Information: We thank all study staff and participants, Venceslau Mambi Pelenda, José Bumba da Cunha, Oliveira Kiatoko, Felismina Caquece, Luzala Elisabeth Armando Garcia, Djos Kialanda, Garcia Pembele, Domingos Jandondo, and Belmira José Bondo. The findings and conclusions in this report are those of the author(s) and do not necessarily represent the official position of the U.S. Centers for Disease Control and Prevention or the U.S. Agency for International Development. The study was funded by the U.S. President’s Malaria Initiative and the Advanced Molecular Detection program at CDC. P.R.D., C.M.F., B.N.A., S.L., R.H., J.F.M.M., F.F., J.F.M., and M.M.P. designed and participated in the specimen collection. P.R.D., A.L.M.C., S.R.R., and J.K. performed the laboratory analysis. P.R.D., A.L.M.C., J.K., S.R.R., J.-H.M.O., and E.T. analyzed the sequencing data. S.R.R. and M.M.P. wrote the manuscript. All authors read and approved the final version of the manuscript. Publisher Copyright: Copyright © 2023 Rosillo et al.
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spelling Molecular Markers of Sulfadoxine-Pyrimethamine Resistance in Samples from Children with Uncomplicated Plasmodium falciparum at Three Sites in Angola in 2019dhfrdhpssurveillanceR MedicineRJ PediatricsQR MicrobiologyQH426 GeneticsPharmacologyPharmacology (medical)Infectious DiseasesParasitologyMolecular BiologySDG 3 - Good Health and Well-beingFunding Information: The study was funded by the U.S. President’s Malaria Initiative and the Advanced Molecular Detection program at CDC. Funding Information: We thank all study staff and participants, Venceslau Mambi Pelenda, José Bumba da Cunha, Oliveira Kiatoko, Felismina Caquece, Luzala Elisabeth Armando Garcia, Djos Kialanda, Garcia Pembele, Domingos Jandondo, and Belmira José Bondo. The findings and conclusions in this report are those of the author(s) and do not necessarily represent the official position of the U.S. Centers for Disease Control and Prevention or the U.S. Agency for International Development. The study was funded by the U.S. President’s Malaria Initiative and the Advanced Molecular Detection program at CDC. P.R.D., C.M.F., B.N.A., S.L., R.H., J.F.M.M., F.F., J.F.M., and M.M.P. designed and participated in the specimen collection. P.R.D., A.L.M.C., S.R.R., and J.K. performed the laboratory analysis. P.R.D., A.L.M.C., J.K., S.R.R., J.-H.M.O., and E.T. analyzed the sequencing data. S.R.R. and M.M.P. wrote the manuscript. All authors read and approved the final version of the manuscript. Publisher Copyright: Copyright © 2023 Rosillo et al.Sulfadoxine-pyrimethamine (SP) is used for prevention of malaria in pregnant women in Angola. We sequenced the Plasmodium falciparum dihydrofolate reductase (pfdhfr) and dihydropteroate synthase (pfdhps) genes, implicated in SP resistance, in samples collected during a 2019 study of artemisinin-based combination therapy efficacy in Benguela, Lunda Sul, and Zaire provinces. A total of 90 day 0 and day of failure samples were individually sequenced, while 508 day 0 samples from participants without recurrent parasitemia were pooled after DNA extraction into 61 pools. The N51I, C59R, and S108N pfdhfr mutations and A437G pfdhps mutations were present at high proportions in all provinces (weighted allele frequencies, 62% to 100%). The K540E pfdhps mutation was present at lower proportions (10% to 14%). The A581G pfdhps mutation was only observed in Zaire, at a 4.6% estimated prevalence. The I431V and A613S mutations were also only observed in Zaire, at a prevalence of 2.8% to 2.9%. The most common (27% to 66%) reconstructed haplotype in all three provinces was the canonical quadruple pfdhfr pfdhps mutant. The canonical quintuple mutant was absent in Lunda Sul and Benguela and present in 7.9% of samples in Zaire. A single canonical sextuple (2.6%) mutant was observed in Zaire Province. Proportions of the pfdhps K540E and A581G mutations were well below the World Health Organization thresholds for meaningful SP resistance (prevalence of 95% for K540E and 10% for A581G). Samples from therapeutic efficacy studies represent a convenient source of samples for monitoring SP resistance markers.Individual Health Care (IHC)Global Health and Tropical Medicine (GHTM)Instituto de Higiene e Medicina Tropical (IHMT)RUNRosillo, Stefano R.Dimbu, Pedro RafaelCândido, Ana Luisa M.Oh, Je Hoon MichaelFerreira, Carolina MiguelAndrade, Benjamin NietoLabuda, SarahHorth, RobertaKelley, JuliaMorais, Joana F.M.Fortes, FilomenoMartins, José FrancoTalundzic, EldinPluciński, Mateusz M.2023-07-11T22:21:13Z2023-042023-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://hdl.handle.net/10362/155095eng0066-4804PURE: 64215533https://doi.org/10.1128/aac.01601-22info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T18:12:45Zoai:run.unl.pt:10362/155095Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:43:24.336726Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Molecular Markers of Sulfadoxine-Pyrimethamine Resistance in Samples from Children with Uncomplicated Plasmodium falciparum at Three Sites in Angola in 2019
title Molecular Markers of Sulfadoxine-Pyrimethamine Resistance in Samples from Children with Uncomplicated Plasmodium falciparum at Three Sites in Angola in 2019
spellingShingle Molecular Markers of Sulfadoxine-Pyrimethamine Resistance in Samples from Children with Uncomplicated Plasmodium falciparum at Three Sites in Angola in 2019
Rosillo, Stefano R.
dhfr
dhps
surveillance
R Medicine
RJ Pediatrics
QR Microbiology
QH426 Genetics
Pharmacology
Pharmacology (medical)
Infectious Diseases
Parasitology
Molecular Biology
SDG 3 - Good Health and Well-being
title_short Molecular Markers of Sulfadoxine-Pyrimethamine Resistance in Samples from Children with Uncomplicated Plasmodium falciparum at Three Sites in Angola in 2019
title_full Molecular Markers of Sulfadoxine-Pyrimethamine Resistance in Samples from Children with Uncomplicated Plasmodium falciparum at Three Sites in Angola in 2019
title_fullStr Molecular Markers of Sulfadoxine-Pyrimethamine Resistance in Samples from Children with Uncomplicated Plasmodium falciparum at Three Sites in Angola in 2019
title_full_unstemmed Molecular Markers of Sulfadoxine-Pyrimethamine Resistance in Samples from Children with Uncomplicated Plasmodium falciparum at Three Sites in Angola in 2019
title_sort Molecular Markers of Sulfadoxine-Pyrimethamine Resistance in Samples from Children with Uncomplicated Plasmodium falciparum at Three Sites in Angola in 2019
author Rosillo, Stefano R.
author_facet Rosillo, Stefano R.
Dimbu, Pedro Rafael
Cândido, Ana Luisa M.
Oh, Je Hoon Michael
Ferreira, Carolina Miguel
Andrade, Benjamin Nieto
Labuda, Sarah
Horth, Roberta
Kelley, Julia
Morais, Joana F.M.
Fortes, Filomeno
Martins, José Franco
Talundzic, Eldin
Pluciński, Mateusz M.
author_role author
author2 Dimbu, Pedro Rafael
Cândido, Ana Luisa M.
Oh, Je Hoon Michael
Ferreira, Carolina Miguel
Andrade, Benjamin Nieto
Labuda, Sarah
Horth, Roberta
Kelley, Julia
Morais, Joana F.M.
Fortes, Filomeno
Martins, José Franco
Talundzic, Eldin
Pluciński, Mateusz M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Individual Health Care (IHC)
Global Health and Tropical Medicine (GHTM)
Instituto de Higiene e Medicina Tropical (IHMT)
RUN
dc.contributor.author.fl_str_mv Rosillo, Stefano R.
Dimbu, Pedro Rafael
Cândido, Ana Luisa M.
Oh, Je Hoon Michael
Ferreira, Carolina Miguel
Andrade, Benjamin Nieto
Labuda, Sarah
Horth, Roberta
Kelley, Julia
Morais, Joana F.M.
Fortes, Filomeno
Martins, José Franco
Talundzic, Eldin
Pluciński, Mateusz M.
dc.subject.por.fl_str_mv dhfr
dhps
surveillance
R Medicine
RJ Pediatrics
QR Microbiology
QH426 Genetics
Pharmacology
Pharmacology (medical)
Infectious Diseases
Parasitology
Molecular Biology
SDG 3 - Good Health and Well-being
topic dhfr
dhps
surveillance
R Medicine
RJ Pediatrics
QR Microbiology
QH426 Genetics
Pharmacology
Pharmacology (medical)
Infectious Diseases
Parasitology
Molecular Biology
SDG 3 - Good Health and Well-being
description Funding Information: The study was funded by the U.S. President’s Malaria Initiative and the Advanced Molecular Detection program at CDC. Funding Information: We thank all study staff and participants, Venceslau Mambi Pelenda, José Bumba da Cunha, Oliveira Kiatoko, Felismina Caquece, Luzala Elisabeth Armando Garcia, Djos Kialanda, Garcia Pembele, Domingos Jandondo, and Belmira José Bondo. The findings and conclusions in this report are those of the author(s) and do not necessarily represent the official position of the U.S. Centers for Disease Control and Prevention or the U.S. Agency for International Development. The study was funded by the U.S. President’s Malaria Initiative and the Advanced Molecular Detection program at CDC. P.R.D., C.M.F., B.N.A., S.L., R.H., J.F.M.M., F.F., J.F.M., and M.M.P. designed and participated in the specimen collection. P.R.D., A.L.M.C., S.R.R., and J.K. performed the laboratory analysis. P.R.D., A.L.M.C., J.K., S.R.R., J.-H.M.O., and E.T. analyzed the sequencing data. S.R.R. and M.M.P. wrote the manuscript. All authors read and approved the final version of the manuscript. Publisher Copyright: Copyright © 2023 Rosillo et al.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-11T22:21:13Z
2023-04
2023-04-01T00:00:00Z
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