Gramicidin D and dithiothreitol effects on erythrocyte exovesiculation

Bibliographic Details
Main Author: Santos, Nuno C.
Publication Date: 2005
Other Authors: Martins-Silva, J., Saldanha, Carlota
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10451/20186
Summary: © Copyright 2005 by Humana Press Inc.
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spelling Gramicidin D and dithiothreitol effects on erythrocyte exovesiculationErythrocyte membrane vesicleGramicidinDithiothreitolCholesterolPhospholipidMembrane fluidity© Copyright 2005 by Humana Press Inc.The use of either diphenylhexatriene, trimethylamino-diphenylhexatriene, or heptadecyl-hydroxy coumarin (C17-HC) allows, simultaneously and with the same molecule, the induction of erythrocyte exovesiculation and labeling of the released vesicles with the fluorescent probe. This method was used to evaluate gramicidin D (a channel-forming peptide) and dithiothreitol (a reducing agent) effects on the human erythrocytes vesiculation process. The release of cholesterol and phospholipids in exovesicles at longer incubation times was only detectable in the presence of gramicidin or dithiothreitol lead to a drastic decrease on the [phospholipids]/[cholesterol] ratio. However, in the samples with dithio-threitol, this variation did not result in the expectable decrease of membrane fluidity. These effects can be related with the presence of lipid rafts, the transbilayer lipids reorientation induced by gramicidin or dithiothreitol, and the cholesterol-dependent gramicidin channels inactivation.This work was supported by the Fundação para a Ciência e Tecnologia (FCT) of the Portuguese Ministry of Science, Innovation, and Higher Education (MCIES).Humana PressRepositório da Universidade de LisboaSantos, Nuno C.Martins-Silva, J.Saldanha, Carlota2015-10-01T11:34:32Z20052005-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/20186engCell Biochemistry and Biophysics December 2005, Volume 43, Issue 3, pp 419-4301085-9195http://dx.doi.org/10.1385/CBB:43:3:419metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T13:20:54Zoai:repositorio.ulisboa.pt:10451/20186Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T02:41:18.735760Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Gramicidin D and dithiothreitol effects on erythrocyte exovesiculation
title Gramicidin D and dithiothreitol effects on erythrocyte exovesiculation
spellingShingle Gramicidin D and dithiothreitol effects on erythrocyte exovesiculation
Santos, Nuno C.
Erythrocyte membrane vesicle
Gramicidin
Dithiothreitol
Cholesterol
Phospholipid
Membrane fluidity
title_short Gramicidin D and dithiothreitol effects on erythrocyte exovesiculation
title_full Gramicidin D and dithiothreitol effects on erythrocyte exovesiculation
title_fullStr Gramicidin D and dithiothreitol effects on erythrocyte exovesiculation
title_full_unstemmed Gramicidin D and dithiothreitol effects on erythrocyte exovesiculation
title_sort Gramicidin D and dithiothreitol effects on erythrocyte exovesiculation
author Santos, Nuno C.
author_facet Santos, Nuno C.
Martins-Silva, J.
Saldanha, Carlota
author_role author
author2 Martins-Silva, J.
Saldanha, Carlota
author2_role author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Santos, Nuno C.
Martins-Silva, J.
Saldanha, Carlota
dc.subject.por.fl_str_mv Erythrocyte membrane vesicle
Gramicidin
Dithiothreitol
Cholesterol
Phospholipid
Membrane fluidity
topic Erythrocyte membrane vesicle
Gramicidin
Dithiothreitol
Cholesterol
Phospholipid
Membrane fluidity
description © Copyright 2005 by Humana Press Inc.
publishDate 2005
dc.date.none.fl_str_mv 2005
2005-01-01T00:00:00Z
2015-10-01T11:34:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/20186
url http://hdl.handle.net/10451/20186
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cell Biochemistry and Biophysics December 2005, Volume 43, Issue 3, pp 419-430
1085-9195
http://dx.doi.org/10.1385/CBB:43:3:419
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Humana Press
publisher.none.fl_str_mv Humana Press
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833601455167111168

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