Development of an indirect ELISA for the serodiagnosis of canine infection by Onchocerca lupi

Bibliographic Details
Main Author: Latrofa, Maria Stefania
Publication Date: 2024
Other Authors: Louzada-Flores, Viviane Noll, Maia, Carla, Kelly, Maureen A, Verocai, Guilherme G, Cantacessi, Cinzia, Otranto, Domenico
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/168743
Summary: © 2024. The Author(s).
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spelling Development of an indirect ELISA for the serodiagnosis of canine infection by Onchocerca lupiImmunology and Microbiology(all)ParasitologyInfectious Diseasesveterinary(all)SDG 3 - Good Health and Well-being© 2024. The Author(s).Onchocerca lupi is a zoonotic filarioid parasite of dogs and cats with widespread distribution. A specific non-invasive diagnostic assay for the detection of O. lupi infections remains unavailable. This study aimed to assess the accuracy, specificity, and sensitivity of an ELISA test designed using nine peptides from two O. lupi proteins. Sera (n = 54) collected from O. lupi infected dogs from endemic areas (Portugal and USA), alongside sera from dogs positive for Dirofilaria immitis, D. repens, Cercopithifilaria bainae, and Acanthocheilonema reconditum (n = 53) from a non-endemic area for O. lupi, as well as from helminth-free dogs (n = 60), were tested. The checkerboard titration method was applied for the optimization of peptide concentrations and conjugate anti-dog dilutions. Sensitivity, specificity, and optimal cut-off values were calculated using ROC curve analysis. All peptides reacted against sera of O. lupi, with no correlation between optic density (OD) values and microfilariae (mfs) loads. Sensitivity and specificity values ranging from 85.45 to 100%, and 88.89% to 100%, respectively, were recorded for all peptides examined, with 100% specificity and sensitivity observed for peptides 40_3, 40_5, 130_3, 120_3 and 40_1, 130_5, respectively. The maximum cut-off value was observed for peptides 40_5 (0.765) and 40_3 (0.708). Testing of sera from dogs positive for other filarioids resulted in lower OD values (up to 1.565) for peptides 40_3 and 40_5 when compared with O. lupi (up to 2.929). The availability of this assay will be of value in epidemiological studies of canine O. lupi infection in both endemic and non-endemic areas, and in assessing the risk for zoonotic transmission.Laboratório Associado de Translacção e Inovação para a Saúde Global - LA Real (Pólo IHMT)Vector borne diseases and pathogens (VBD)Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)RUNLatrofa, Maria StefaniaLouzada-Flores, Viviane NollMaia, CarlaKelly, Maureen AVerocai, Guilherme GCantacessi, CinziaOtranto, Domenico2024-06-19T00:12:02Z2024-02-092024-02-09T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/168743eng2045-2322PURE: 83389720https://doi.org/10.1038/s41598-024-53759-winfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-31T01:52:38Zoai:run.unl.pt:10362/168743Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:56:19.674059Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Development of an indirect ELISA for the serodiagnosis of canine infection by Onchocerca lupi
title Development of an indirect ELISA for the serodiagnosis of canine infection by Onchocerca lupi
spellingShingle Development of an indirect ELISA for the serodiagnosis of canine infection by Onchocerca lupi
Latrofa, Maria Stefania
Immunology and Microbiology(all)
Parasitology
Infectious Diseases
veterinary(all)
SDG 3 - Good Health and Well-being
title_short Development of an indirect ELISA for the serodiagnosis of canine infection by Onchocerca lupi
title_full Development of an indirect ELISA for the serodiagnosis of canine infection by Onchocerca lupi
title_fullStr Development of an indirect ELISA for the serodiagnosis of canine infection by Onchocerca lupi
title_full_unstemmed Development of an indirect ELISA for the serodiagnosis of canine infection by Onchocerca lupi
title_sort Development of an indirect ELISA for the serodiagnosis of canine infection by Onchocerca lupi
author Latrofa, Maria Stefania
author_facet Latrofa, Maria Stefania
Louzada-Flores, Viviane Noll
Maia, Carla
Kelly, Maureen A
Verocai, Guilherme G
Cantacessi, Cinzia
Otranto, Domenico
author_role author
author2 Louzada-Flores, Viviane Noll
Maia, Carla
Kelly, Maureen A
Verocai, Guilherme G
Cantacessi, Cinzia
Otranto, Domenico
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Laboratório Associado de Translacção e Inovação para a Saúde Global - LA Real (Pólo IHMT)
Vector borne diseases and pathogens (VBD)
Instituto de Higiene e Medicina Tropical (IHMT)
Global Health and Tropical Medicine (GHTM)
RUN
dc.contributor.author.fl_str_mv Latrofa, Maria Stefania
Louzada-Flores, Viviane Noll
Maia, Carla
Kelly, Maureen A
Verocai, Guilherme G
Cantacessi, Cinzia
Otranto, Domenico
dc.subject.por.fl_str_mv Immunology and Microbiology(all)
Parasitology
Infectious Diseases
veterinary(all)
SDG 3 - Good Health and Well-being
topic Immunology and Microbiology(all)
Parasitology
Infectious Diseases
veterinary(all)
SDG 3 - Good Health and Well-being
description © 2024. The Author(s).
publishDate 2024
dc.date.none.fl_str_mv 2024-06-19T00:12:02Z
2024-02-09
2024-02-09T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/168743
url http://hdl.handle.net/10362/168743
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2045-2322
PURE: 83389720
https://doi.org/10.1038/s41598-024-53759-w
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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