Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylation

Bibliographic Details
Main Author: Saldanha, Carlota
Publication Date: 2007
Other Authors: Silva, Ana S., Gonçalves, Sónia, Martins-Silva, João
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10451/20193
Summary: © 2007 – IOS Press and the authors. All rights reserved
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spelling Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylationBand 3ErythrocytePhosphorylationDephosphorylationProtein tyrosine kinaseProtein tyrosine phosphatase© 2007 – IOS Press and the authors. All rights reservedThe objective of the present work was to study the effects of erythrocyte proteins phosphorylation in erythrocyte aggregation and deformability. Human whole blood samples were incubated in vitro in absent and in presence of the phosphorylation/dephosphorilation band 3 inhibitors and also with adenylyl cyclase, guanylate cyclase and PI3K inhibitors and the erythrocyte aggregation index (EAI) and deformability were assayed. The results show that when band 3 is phosphorylated in presence of a PTP inhibitor an increase in erythrocyte aggregation index is observed (p < 0.0001). A partial dephosphorylation band 3 state, induced by PTK inhibitors, show a decrease in the erythrocyte aggregation index (p < 0.002). However both manipulated states induced lower EAI values than blood samples aliquots controls. The guanylate cyclase and PI3-K inhibitors significantly decrease the erythrocyte aggregation index in relation with the control blood samples. Erythrocyte deformability in presence of all the inhibitors did not showed significant changes. PTP and PI3-K inhibitors showed a significantly increase in the plasma potassium concentrations not associated with EAI values. Methehemoglobin levels were increased significantly when guanylate cyclase inhibitor is present in the blood samples. In conclusion, the results suggest that erythrocyte aggregation index is dependent of the phosphorylated/dephosphorylated state of band 3.IOS PressRepositório da Universidade de LisboaSaldanha, CarlotaSilva, Ana S.Gonçalves, SóniaMartins-Silva, João2015-10-01T15:48:52Z20072007-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/20193engClinical Hemorheology and Microcirculation 36 (2007) 183–1941386-0291http://content.iospress.com/articles/clinical-hemorheology-and-microcirculation/ch969info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T13:20:55Zoai:repositorio.ulisboa.pt:10451/20193Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T02:41:19.046559Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylation
title Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylation
spellingShingle Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylation
Saldanha, Carlota
Band 3
Erythrocyte
Phosphorylation
Dephosphorylation
Protein tyrosine kinase
Protein tyrosine phosphatase
title_short Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylation
title_full Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylation
title_fullStr Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylation
title_full_unstemmed Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylation
title_sort Modulation of erythrocyte hemorheological properties by band 3 phosphorylation and dephosphorylation
author Saldanha, Carlota
author_facet Saldanha, Carlota
Silva, Ana S.
Gonçalves, Sónia
Martins-Silva, João
author_role author
author2 Silva, Ana S.
Gonçalves, Sónia
Martins-Silva, João
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Saldanha, Carlota
Silva, Ana S.
Gonçalves, Sónia
Martins-Silva, João
dc.subject.por.fl_str_mv Band 3
Erythrocyte
Phosphorylation
Dephosphorylation
Protein tyrosine kinase
Protein tyrosine phosphatase
topic Band 3
Erythrocyte
Phosphorylation
Dephosphorylation
Protein tyrosine kinase
Protein tyrosine phosphatase
description © 2007 – IOS Press and the authors. All rights reserved
publishDate 2007
dc.date.none.fl_str_mv 2007
2007-01-01T00:00:00Z
2015-10-01T15:48:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/20193
url http://hdl.handle.net/10451/20193
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clinical Hemorheology and Microcirculation 36 (2007) 183–194
1386-0291
http://content.iospress.com/articles/clinical-hemorheology-and-microcirculation/ch969
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv IOS Press
publisher.none.fl_str_mv IOS Press
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833601455177596928

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