Development of electrochemical oxidase biosensors based on carbon nanotube-modified carbon film electrodes for glucose and ethanol

• Main Author: Gouveia-Caridade, Carla
• Publication Date: 2008
• Other Authors: Pauliukaite, Rasa, Brett, Christopher M. A.
• Format: Article
• Language: eng
• Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
• Download full: https://hdl.handle.net/10316/5285; https://doi.org/10.1016/j.electacta.2008.01.040
• topic: Multi-walled carbon nanotubes; Carbon film electrodes; Glucose oxidase; Alcohol oxidase; Electrochemical biosensor

Functionalised multi-walled carbon nanotubes (MWCNTs) were cast on glassy carbon (GC) and carbon film electrodes (CFE), and were characterised electrochemically and applied in a glucose-oxidase-based biosensor. MWCNT-modified carbon film electrodes were then used to develop an alcohol oxidase (AlcOx) biosensor, in which AlcOx-BSA was cross-linked with glutaraldehyde and attached by drop-coating. The experimental conditions, applied potential and pH, for ethanol monitoring were optimised, and ethanol was determined amperometrically at -0.3 V vs. SCE at pH 7.5. Electrocatalytic effects of MWCNT were observed with respect to unmodified carbon film electrodes. The sensitivity obtained was 20 times higher at carbon film/MWCNT-based biosensors than without MWCNT.