Aquaporins in adipocytes:insights into the pathophysiology of human obesity

Bibliographic Details
Main Author: Madeira, Ana Paula da Silva
Publication Date: 2014
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10451/10740
Summary: Tese de doutoramento, Farmácia (Bioquímica), Universidade de Lisboa, Faculdade de Farmácia, 2014
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spelling Aquaporins in adipocytes:insights into the pathophysiology of human obesityTeses de doutoramento - 2014Tese de doutoramento, Farmácia (Bioquímica), Universidade de Lisboa, Faculdade de Farmácia, 2014Adipocyte glycerol permeability has been appointed as a novel regulator of adipocyte metabolism and whole body fat mass. Up to now, Aquaporin-7 (AQP7) has been the mainly characterized protein in adipocytes with known ability to transport glycerol. However, the glycerol pathways in adipocytes are not yet fully understood. For these reasons AQP7 activity was further characterized in adipocytes, and its water and glycerol permeabilities were determined. The regulatory mechanisms of water and glycerol permeability in adipocytes were also investigated, being given special emphasis to the regulation of AQP7. Within the search for alternative glycerol channels in mice and human adipocytes, it was also investigated the presence of unexpected aquaporins in these cells. Our studies led us to conclude that both human and mouse AQP7 are functional water and glycerol channels when expressed in adipocytes. Regarding the mechanisms of AQP7 modulation it was detected the inhibition of both mice and human AQP7 activity by a gold (III) coordination compound. Methionine residues have been identified as possible binding sites for gold ions. It was also tested the effect of dietary conjugated linoleic acid (CLA) on adipose cells membrane permeability to water and glycerol. It was observed that CLA supplementation in obese Zucker rats reduces the fluidity and permeability of adipose membranes, whereas in 3T3-L1, CLA supplementation during adipocytes differentiation increases glycerol permeability. The search for novel aquaporins in adipose tissue led us to detect AQP11 in both subcutaneous and visceral human mature adipocytes. Moreover, it was observed that AQP11 is primarily located intracellularly in human adipocytes and that it partially colocalizes with perilipin, pointing towards AQP11 preferential location in the vicinity of lipid droplets. Very importantly, it was described for the first time the specificity of human AQP11 for both water and glycerol when expressed in adipocytes.Fundação para a Ciência e a Tecnologia (FCT, SFRH/BD/45930/2008)Rodrigues, Maria da Graça Soveral, 1959-Zorzano, AntónioRepositório da Universidade de LisboaMadeira, Ana Paula da Silva2014-03-14T12:14:58Z20142014-01-01T00:00:00Zdoctoral thesisinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10451/10740TID:101305729enginfo:eu-repo/semantics/embargoedAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T13:06:42Zoai:repositorio.ulisboa.pt:10451/10740Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T02:35:09.527909Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Aquaporins in adipocytes:insights into the pathophysiology of human obesity
title Aquaporins in adipocytes:insights into the pathophysiology of human obesity
spellingShingle Aquaporins in adipocytes:insights into the pathophysiology of human obesity
Madeira, Ana Paula da Silva
Teses de doutoramento - 2014
title_short Aquaporins in adipocytes:insights into the pathophysiology of human obesity
title_full Aquaporins in adipocytes:insights into the pathophysiology of human obesity
title_fullStr Aquaporins in adipocytes:insights into the pathophysiology of human obesity
title_full_unstemmed Aquaporins in adipocytes:insights into the pathophysiology of human obesity
title_sort Aquaporins in adipocytes:insights into the pathophysiology of human obesity
author Madeira, Ana Paula da Silva
author_facet Madeira, Ana Paula da Silva
author_role author
dc.contributor.none.fl_str_mv Rodrigues, Maria da Graça Soveral, 1959-
Zorzano, António
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Madeira, Ana Paula da Silva
dc.subject.por.fl_str_mv Teses de doutoramento - 2014
topic Teses de doutoramento - 2014
description Tese de doutoramento, Farmácia (Bioquímica), Universidade de Lisboa, Faculdade de Farmácia, 2014
publishDate 2014
dc.date.none.fl_str_mv 2014-03-14T12:14:58Z
2014
2014-01-01T00:00:00Z
dc.type.driver.fl_str_mv doctoral thesis
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/10740
TID:101305729
url http://hdl.handle.net/10451/10740
identifier_str_mv TID:101305729
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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