Antimicrobial activity of a bacterial nanocellulose film functionalized with nisin Z for prospective burn wounds treatment
| Main Author: | |
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| Publication Date: | 2022 |
| Other Authors: | , , , , , , , |
| Language: | eng |
| Source: | Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
| Download full: | https://hdl.handle.net/1822/80976 |
Summary: | Supplementary Materials: The following are available online at https://www.mdpi.com/article/10 .3390/eca2022-12708/s1 |
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Antimicrobial activity of a bacterial nanocellulose film functionalized with nisin Z for prospective burn wounds treatmentantimicrobial peptidesNisin Zbacterial nanocellulosewound dressingSupplementary Materials: The following are available online at https://www.mdpi.com/article/10 .3390/eca2022-12708/s1Burn wounds can lead to numerous severe complications including bacterial infections causing patient morbidity and mortality, mostly in low- and middle-income countries. The considerable increase in microbial resistance against traditional antibiotics is leading towards alternative strategies to treat bacterial infections. Nisin Z is an antimicrobial peptide which exhibits a significant antibacterial activity against Gram-positive bacteria. Its efficacy against Gram-negative bacteria is limited, nonetheless it can be improved with the addition of surfactants, such as ethylenediaminetetraacetic acid (EDTA). The incorporation of peptide and other biomolecules within a biopolymer matrix provides protection maintaining their antimicrobial potential. Bacterial nanocellulose (BNC) has been widely used as wound dressings. Its impressive water retention capacity (>99%) and porosity are beneficial to manage wounds due to its potential to absorb exudates, providing a breathable and humid environment. In this work, the functionalization of BNC with Nisin Z (BNC-NZ) via vacuum filtration is reported. The entrapment of the peptide inside the BNC films was confirmed through morphological characterization using attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectrometry. Typical absorbance peaks of Nisin Z are easily identifiable at 1647 cm−1 (amide group) and 1520 cm−1 (bending of primary amines). Thermal gravimetric analysis (TGA) suggested that Nisin Z did not interfere with the BNC matrix. The antimicrobial activity of Nisin Z against five of the most common bacteria found in burn wounds was verified by minimum bactericidal concentration (MBC) ranging 8.0–256.0 µg/mL. Agar diffusion and shake flask methods revealed the potential of BNC-NZ for prospective applications in burn wound dressings.This research was funded by FEDER funds through COMPETE and by national funds through FCT via the projects POCI-01-0145-FEDER-028074 and UID/CTM/00264/2020. L.M. and T.D.T. also acknowledge FCT for their Ph.D. scholarships with references 2020.04919.BD and 2020.06046.BD.info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoMelro, LilianaTavares, Tânia D.Padrão, JorgeDourado, FernandoGama, F. M.Silva, C.Antunes, Joana C.Felgueiras, Helena PradoZille, Andrea2022-06-152022-06-15T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/1822/80976engMelro, Liliana; Tavares, Tânia D.; Padrão, Jorge; Dourado, Fernando; Gama, F. M.; Silva, Carla; Antunes, Joana C.; Felgueiras, Helena P.; Zille, Andrea, Antimicrobial activity of a bacterial nanocellulose film functionalized with nisin Z for prospective burn wounds treatment. Medical Sciences Forum. Vol. 12(1), MDPI AG, 1, 2022.2673-999210.3390/eca2022-12708https://www.mdpi.com/2673-9992/12/1/1info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T06:48:08Zoai:repositorium.sdum.uminho.pt:1822/80976Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:04:48.534623Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse |
| dc.title.none.fl_str_mv |
Antimicrobial activity of a bacterial nanocellulose film functionalized with nisin Z for prospective burn wounds treatment |
| title |
Antimicrobial activity of a bacterial nanocellulose film functionalized with nisin Z for prospective burn wounds treatment |
| spellingShingle |
Antimicrobial activity of a bacterial nanocellulose film functionalized with nisin Z for prospective burn wounds treatment Melro, Liliana antimicrobial peptides Nisin Z bacterial nanocellulose wound dressing |
| title_short |
Antimicrobial activity of a bacterial nanocellulose film functionalized with nisin Z for prospective burn wounds treatment |
| title_full |
Antimicrobial activity of a bacterial nanocellulose film functionalized with nisin Z for prospective burn wounds treatment |
| title_fullStr |
Antimicrobial activity of a bacterial nanocellulose film functionalized with nisin Z for prospective burn wounds treatment |
| title_full_unstemmed |
Antimicrobial activity of a bacterial nanocellulose film functionalized with nisin Z for prospective burn wounds treatment |
| title_sort |
Antimicrobial activity of a bacterial nanocellulose film functionalized with nisin Z for prospective burn wounds treatment |
| author |
Melro, Liliana |
| author_facet |
Melro, Liliana Tavares, Tânia D. Padrão, Jorge Dourado, Fernando Gama, F. M. Silva, C. Antunes, Joana C. Felgueiras, Helena Prado Zille, Andrea |
| author_role |
author |
| author2 |
Tavares, Tânia D. Padrão, Jorge Dourado, Fernando Gama, F. M. Silva, C. Antunes, Joana C. Felgueiras, Helena Prado Zille, Andrea |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
Melro, Liliana Tavares, Tânia D. Padrão, Jorge Dourado, Fernando Gama, F. M. Silva, C. Antunes, Joana C. Felgueiras, Helena Prado Zille, Andrea |
| dc.subject.por.fl_str_mv |
antimicrobial peptides Nisin Z bacterial nanocellulose wound dressing |
| topic |
antimicrobial peptides Nisin Z bacterial nanocellulose wound dressing |
| description |
Supplementary Materials: The following are available online at https://www.mdpi.com/article/10 .3390/eca2022-12708/s1 |
| publishDate |
2022 |
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2022-06-15 2022-06-15T00:00:00Z |
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conference object |
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info:eu-repo/semantics/publishedVersion |
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publishedVersion |
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https://hdl.handle.net/1822/80976 |
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https://hdl.handle.net/1822/80976 |
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eng |
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eng |
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Melro, Liliana; Tavares, Tânia D.; Padrão, Jorge; Dourado, Fernando; Gama, F. M.; Silva, Carla; Antunes, Joana C.; Felgueiras, Helena P.; Zille, Andrea, Antimicrobial activity of a bacterial nanocellulose film functionalized with nisin Z for prospective burn wounds treatment. Medical Sciences Forum. Vol. 12(1), MDPI AG, 1, 2022. 2673-9992 10.3390/eca2022-12708 https://www.mdpi.com/2673-9992/12/1/1 |
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openAccess |
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application/pdf |
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MDPI |
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MDPI |
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