Carbon monoxide, autophagy and cytoprotection in response to cerebral ischemia

Detalhes bibliográficos
Autor(a) principal: Pereira, Claudia Figueiredo
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: http://hdl.handle.net/10362/10836
Resumo: Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina
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spelling Carbon monoxide, autophagy and cytoprotection in response to cerebral ischemiaAstrocytesNeuroprotectionCarbon monoxideCORM’sAutophagyMitophagyDissertação para obtenção do Grau de Mestre em Genética Molecular e BiomedicinaThere is an increasing need for promoting neuroprotection against cerebral ischemia, which is the main cause of brain damage in adults. Astrocytes are the most abundant cells inboard the central nervous system (CNS), being known as key glial cell for promoting neuronal survival and homeostasis. It is more established in nowdays that astrocytic dysfunction contributes to neurodegenerative processes. Although, carbon monoxide is a well renown as a lethal and toxic gas due to its high affinity to hemoglobin, CO exerts anti-apoptotic, anti-inflammatory and anti-proliferative functions. Recent studies showed likewise that CO induces autophagy, promoting therefore cytoprotective and anti-inflammatory effects. Autophagy is a major catabolic pathway, known as an autodigestive process that degrades cellular organelles and proteins, playing an important role in cellular homeostasis during environmental stress. Due to the great interest on the signaling and cytoprotective actions of CO, novel strategies have been put forward to exploit the potential therapeutic effects of this gaseous molecule. One of these approaches consist on the development of CO-releasing molecules (CO-RMs), compounds that deliver small quantities of CO to tissues and first identified by the group of Motterlini and co-workers. The aim of this Master thesis was to study the action of CORM-A1, a boron-containing compound that spontaneously releases CO, against cell death in primary culture of astrocytes. In particular, we examined the role of CORM-A1 in autophagy, mitophagy and cell metabolism. Here, we demonstrated that CORM-A1 promotes the induction of autophagy in primary culture of astrocytes. Furthermore,autophagy is directly involved in the cytoprotective effect of CORM-A1 against cell death. In some preliminary experiments we have shown that CORM-A1 also induced mitophagy, while autophagy and inhibition of cell death promoted by CORM-A1 seem to occur under hypoxia (5% of oxygen). This master thesis has addressed several important questions on the role of CO in astrocyte function but also opened to many other important questions on the mechanism of action of CO. For instance, future work must be undertaken in order to explore whether CO-mediated induction of reactive oxygen species (ROS), which play an important role in cell signaling, which are the factors directly involved in mitophagy and the cross-talk between apoptosis and modulation of autophagy.e-COST,(COST Action BM1005)Faculdade de Ciências e TecnologiaVieira, HelenaRUNPereira, Claudia Figueiredo20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/10836enginfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:14:39Zoai:run.unl.pt:10362/10836Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T16:45:42.702158Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Carbon monoxide, autophagy and cytoprotection in response to cerebral ischemia
title Carbon monoxide, autophagy and cytoprotection in response to cerebral ischemia
spellingShingle Carbon monoxide, autophagy and cytoprotection in response to cerebral ischemia
Pereira, Claudia Figueiredo
Astrocytes
Neuroprotection
Carbon monoxide
CORM’s
Autophagy
Mitophagy
title_short Carbon monoxide, autophagy and cytoprotection in response to cerebral ischemia
title_full Carbon monoxide, autophagy and cytoprotection in response to cerebral ischemia
title_fullStr Carbon monoxide, autophagy and cytoprotection in response to cerebral ischemia
title_full_unstemmed Carbon monoxide, autophagy and cytoprotection in response to cerebral ischemia
title_sort Carbon monoxide, autophagy and cytoprotection in response to cerebral ischemia
author Pereira, Claudia Figueiredo
author_facet Pereira, Claudia Figueiredo
author_role author
dc.contributor.none.fl_str_mv Vieira, Helena
RUN
dc.contributor.author.fl_str_mv Pereira, Claudia Figueiredo
dc.subject.por.fl_str_mv Astrocytes
Neuroprotection
Carbon monoxide
CORM’s
Autophagy
Mitophagy
topic Astrocytes
Neuroprotection
Carbon monoxide
CORM’s
Autophagy
Mitophagy
description Dissertação para obtenção do Grau de Mestre em Genética Molecular e Biomedicina
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/10836
url http://hdl.handle.net/10362/10836
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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