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Exploring respiratory enzymes from Staphylococcus aureus

Detalhes bibliográficos
Autor(a) principal: Barbosa, Catarina
Data de Publicação: 2018
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: http://hdl.handle.net/10362/130064
Resumo: "Staphylococcus aureus is an opportunistic pathogen that can cause various disease patterns due to its high adaptative capacity. Many aspects of the energy metabolism and its respiratory chain system are still poorly understood. Herein, we report our study of three respiratory enzymes: Membrane potential- generating system (MpsAB) and two malate:quinone oxidoreductases (MQO). The MpsAB enzyme is a membrane complex proposed to be involved in energy conservation in S. aureus due the sequence homology of the subunit MpsA with the proton-translocation subunit NuoL of complex I from Escherichia coli. To further investigate the complex a series of expression tests were made to achieve its heterologous expression in E. coli. We also tried to optimize protein purification for biochemical studies. (...)"
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spelling Exploring respiratory enzymes from Staphylococcus aureusEnergy MetabolismRespiratory ChainMembrane Potential-Generating System ABMalate:quinone OxidoreductasesBiochemistry for Health"Staphylococcus aureus is an opportunistic pathogen that can cause various disease patterns due to its high adaptative capacity. Many aspects of the energy metabolism and its respiratory chain system are still poorly understood. Herein, we report our study of three respiratory enzymes: Membrane potential- generating system (MpsAB) and two malate:quinone oxidoreductases (MQO). The MpsAB enzyme is a membrane complex proposed to be involved in energy conservation in S. aureus due the sequence homology of the subunit MpsA with the proton-translocation subunit NuoL of complex I from Escherichia coli. To further investigate the complex a series of expression tests were made to achieve its heterologous expression in E. coli. We also tried to optimize protein purification for biochemical studies. (...)"Pereira, ManuelaRefojo, AnaRUNBarbosa, Catarina2022-01-01T22:41:42Z2018-12-062018-102018-12-06T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/130064enginfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:57:49Zoai:run.unl.pt:10362/130064Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:29:05.061342Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Exploring respiratory enzymes from Staphylococcus aureus
title Exploring respiratory enzymes from Staphylococcus aureus
spellingShingle Exploring respiratory enzymes from Staphylococcus aureus
Barbosa, Catarina
Energy Metabolism
Respiratory Chain
Membrane Potential-Generating System AB
Malate:quinone Oxidoreductases
Biochemistry for Health
title_short Exploring respiratory enzymes from Staphylococcus aureus
title_full Exploring respiratory enzymes from Staphylococcus aureus
title_fullStr Exploring respiratory enzymes from Staphylococcus aureus
title_full_unstemmed Exploring respiratory enzymes from Staphylococcus aureus
title_sort Exploring respiratory enzymes from Staphylococcus aureus
author Barbosa, Catarina
author_facet Barbosa, Catarina
author_role author
dc.contributor.none.fl_str_mv Pereira, Manuela
Refojo, Ana
RUN
dc.contributor.author.fl_str_mv Barbosa, Catarina
dc.subject.por.fl_str_mv Energy Metabolism
Respiratory Chain
Membrane Potential-Generating System AB
Malate:quinone Oxidoreductases
Biochemistry for Health
topic Energy Metabolism
Respiratory Chain
Membrane Potential-Generating System AB
Malate:quinone Oxidoreductases
Biochemistry for Health
description "Staphylococcus aureus is an opportunistic pathogen that can cause various disease patterns due to its high adaptative capacity. Many aspects of the energy metabolism and its respiratory chain system are still poorly understood. Herein, we report our study of three respiratory enzymes: Membrane potential- generating system (MpsAB) and two malate:quinone oxidoreductases (MQO). The MpsAB enzyme is a membrane complex proposed to be involved in energy conservation in S. aureus due the sequence homology of the subunit MpsA with the proton-translocation subunit NuoL of complex I from Escherichia coli. To further investigate the complex a series of expression tests were made to achieve its heterologous expression in E. coli. We also tried to optimize protein purification for biochemical studies. (...)"
publishDate 2018
dc.date.none.fl_str_mv 2018-12-06
2018-10
2018-12-06T00:00:00Z
2022-01-01T22:41:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/130064
url http://hdl.handle.net/10362/130064
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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