On the use of dexamethasone-loaded liposomes to induce the osteogenic differentiation of human mesenchymal stem cells

Bibliographic Details
Main Author: Monteiro, Nelson
Publication Date: 2015
Other Authors: Martins, Albino, Ribeiro, Diana Margarida da Costa, Faria, Susana, Fonseca, Nuno A., Moreira, João N., Reis, R. L., Neves, N. M.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/1822/26927
Summary: Article first published online: 7 Oct. 2013
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spelling On the use of dexamethasone-loaded liposomes to induce the osteogenic differentiation of human mesenchymal stem cellsBioactive agentDelivery systemDexamethasoneLiposomeNanoparticlestem cell differentiationScience & TechnologyArticle first published online: 7 Oct. 2013Stem cells have received considerable attention by the scientific community because of their potential for tissue engineering and regenerative medicine. The most frequently used method to promote their differentiation is supplementation of the in vitro culture medium with growth/differentiation factors (GDFs). The limitations of that strategy caused by the short half-life of GDFs limit its efficacy in vivo and consequently its clinical use. Thus, the development of new concepts that enable the bioactivity and bioavailability of GDFs to be protected, both in vitro and in vivo, is very relevant. Nanoparticle-based drug delivery systems can be injected, protect the GDFs and enable spatiotemporal release kinetics to be controlled. Liposomes are well-established nanodelivery devices presenting significant advantages, viz. a high load-carrying capacity, relative safety and easy production, and a versatile nature in terms of possible formulations and surface functionalization. The main objective of the present study was to optimize the formulation of liposomes to encapsulate dexamethasone (Dex). Our results showed that the optimized Dex-loaded liposomes do not have any cytotoxic effect on human bone marrow-derived mesenchymal stem cells (hBMSCs). More importantly, they were able to promote an earlier induction of differentiation of hBMSCs into the osteogenic lineage, as demonstrated by the expression of osteoblastic markers, both phenotypically and genotypically. We concluded that Dex-loaded liposomes represent a viable nanoparticle strategy with enhanced safety and efficacy for tissue engineering and regenerative medicine.The authors thank the Portuguese Foundation for Science and Technology for a PhD grant (No. SFRH/BD/62465/2009, to N. S. Monteiro). This work was partly supported by the FIND and BIND Project (No. NMP4-SL-2009-229292) and the OsteoGraphy Project (No. PTDC/EME-MFE/2008).John Wiley and SonsUniversidade do MinhoMonteiro, NelsonMartins, AlbinoRibeiro, Diana Margarida da CostaFaria, SusanaFonseca, Nuno A.Moreira, João N.Reis, R. L.Neves, N. M.20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/26927engMonteiro, N., Martins, A., Ribeiro, D., Faria, S., Fonseca, N. A., Moreira, J. N., . . . Neves, N. M. (2015). On the use of dexamethasone-loaded liposomes to induce the osteogenic differentiation of human mesenchymal stem cells. Journal of Tissue Engineering and Regenerative Medicine, 9(9), 1056-1066. doi: 10.1002/term.18171932-700510.1002/term.181724123949http://onlinelibrary.wiley.com/doi/10.1002/term.1817/abstractinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T05:42:20Zoai:repositorium.sdum.uminho.pt:1822/26927Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:27:23.219589Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv On the use of dexamethasone-loaded liposomes to induce the osteogenic differentiation of human mesenchymal stem cells
title On the use of dexamethasone-loaded liposomes to induce the osteogenic differentiation of human mesenchymal stem cells
spellingShingle On the use of dexamethasone-loaded liposomes to induce the osteogenic differentiation of human mesenchymal stem cells
Monteiro, Nelson
Bioactive agent
Delivery system
Dexamethasone
Liposome
Nanoparticle
stem cell differentiation
Science & Technology
title_short On the use of dexamethasone-loaded liposomes to induce the osteogenic differentiation of human mesenchymal stem cells
title_full On the use of dexamethasone-loaded liposomes to induce the osteogenic differentiation of human mesenchymal stem cells
title_fullStr On the use of dexamethasone-loaded liposomes to induce the osteogenic differentiation of human mesenchymal stem cells
title_full_unstemmed On the use of dexamethasone-loaded liposomes to induce the osteogenic differentiation of human mesenchymal stem cells
title_sort On the use of dexamethasone-loaded liposomes to induce the osteogenic differentiation of human mesenchymal stem cells
author Monteiro, Nelson
author_facet Monteiro, Nelson
Martins, Albino
Ribeiro, Diana Margarida da Costa
Faria, Susana
Fonseca, Nuno A.
Moreira, João N.
Reis, R. L.
Neves, N. M.
author_role author
author2 Martins, Albino
Ribeiro, Diana Margarida da Costa
Faria, Susana
Fonseca, Nuno A.
Moreira, João N.
Reis, R. L.
Neves, N. M.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Monteiro, Nelson
Martins, Albino
Ribeiro, Diana Margarida da Costa
Faria, Susana
Fonseca, Nuno A.
Moreira, João N.
Reis, R. L.
Neves, N. M.
dc.subject.por.fl_str_mv Bioactive agent
Delivery system
Dexamethasone
Liposome
Nanoparticle
stem cell differentiation
Science & Technology
topic Bioactive agent
Delivery system
Dexamethasone
Liposome
Nanoparticle
stem cell differentiation
Science & Technology
description Article first published online: 7 Oct. 2013
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/26927
url http://hdl.handle.net/1822/26927
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Monteiro, N., Martins, A., Ribeiro, D., Faria, S., Fonseca, N. A., Moreira, J. N., . . . Neves, N. M. (2015). On the use of dexamethasone-loaded liposomes to induce the osteogenic differentiation of human mesenchymal stem cells. Journal of Tissue Engineering and Regenerative Medicine, 9(9), 1056-1066. doi: 10.1002/term.1817
1932-7005
10.1002/term.1817
24123949
http://onlinelibrary.wiley.com/doi/10.1002/term.1817/abstract
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley and Sons
publisher.none.fl_str_mv John Wiley and Sons
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833595325955178496

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