The phototrophic metabolic behaviour of Candidatus accumulibacter

Detalhes bibliográficos
Autor(a) principal: Carvalho, V. C.F.
Data de Publicação: 2024
Outros Autores: Gan, A. Z.M., Shon, A., Kolakovic, S., Freitas, E. B., Reis, M. A.M., Fradinho, J. C., Oehmen, A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: http://hdl.handle.net/10362/178417
Resumo: Funding Information: The authors also acknowledge the Australian Research Council (ARC) through project LP190100329 . Publisher Copyright: © 2024 The Authors
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spelling The phototrophic metabolic behaviour of Candidatus accumulibacterCandidatus Accumulibacter (Accumulibacter)Enhanced biological phosphorus removal (EBPR)Phototrophic growthPolyphosphate accumulating organisms (PAOs)Environmental EngineeringCivil and Structural EngineeringEcological ModellingWater Science and TechnologyWaste Management and DisposalPollutionFunding Information: The authors also acknowledge the Australian Research Council (ARC) through project LP190100329 . Publisher Copyright: © 2024 The AuthorsThe phototrophic capability of Candidatus Accumulibacter (Accumulibacter), a common polyphosphate accumulating organism (PAO) in enhanced biological phosphorus removal (EBPR) systems, was investigated in this study. Accumulibacter is phylogenetically related to the purple bacteria Rhodocyclus from the family Rhodocyclaceae, which belongs to the class Betaproteobacteria. Rhodocyclus typically exhibits both chemoheterotrophic and phototrophic growth, however, limited studies have evaluated the phototrophic potential of Accumulibacter. To address this gap, short and extended light cycle tests were conducted using a highly enriched Accumulibacter culture (95%) to evaluate its responses to illumination. Results showed that, after an initial period of adaptation to light conditions (approximately 4–5 h), Accumulibacter exhibited complete phosphorus (P) uptake by utilising polyhydroxyalkanoates (PHA), and additionally by consuming glycogen, which contrasted with its typical aerobic metabolism. Mass, energy, and redox balance analyses demonstrated that Accumulibacter needed to employ phototrophic metabolism to meet its energy requirements. Calculations revealed that the light reactions contributed to the generation of, at least more than 67% of the ATP necessary for P uptake and growth. Extended light tests, spanning 21 days with dark/light cycles, suggested that Accumulibacter generated ATP through light during initial operation, however, it likely reverted to conventional anaerobic/aerobic metabolism under dark/light conditions due to microalgal growth in the mixed culture, contributing to oxygen production. In contrast, extended light tests with an enriched Tetrasphaera culture, lacking phototrophic genes in its genome, clearly demonstrated that phototrophic P uptake did not occur. These findings highlight the adaptive metabolic capabilities of Accumulibacter, enabling it to utilise phototrophic pathways for energy generation during oxygen deprivation, which holds the potential to advance phototrophic-EBPR technology development.UCIBIO - Applied Molecular Biosciences UnitRUNCarvalho, V. C.F.Gan, A. Z.M.Shon, A.Kolakovic, S.Freitas, E. B.Reis, M. A.M.Fradinho, J. C.Oehmen, A.2025-02-04T21:17:34Z2024-08-012024-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/178417eng0043-1354PURE: 106563394https://doi.org/10.1016/j.watres.2024.121865info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-02-10T01:38:40Zoai:run.unl.pt:10362/178417Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T19:46:48.072404Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv The phototrophic metabolic behaviour of Candidatus accumulibacter
title The phototrophic metabolic behaviour of Candidatus accumulibacter
spellingShingle The phototrophic metabolic behaviour of Candidatus accumulibacter
Carvalho, V. C.F.
Candidatus Accumulibacter (Accumulibacter)
Enhanced biological phosphorus removal (EBPR)
Phototrophic growth
Polyphosphate accumulating organisms (PAOs)
Environmental Engineering
Civil and Structural Engineering
Ecological Modelling
Water Science and Technology
Waste Management and Disposal
Pollution
title_short The phototrophic metabolic behaviour of Candidatus accumulibacter
title_full The phototrophic metabolic behaviour of Candidatus accumulibacter
title_fullStr The phototrophic metabolic behaviour of Candidatus accumulibacter
title_full_unstemmed The phototrophic metabolic behaviour of Candidatus accumulibacter
title_sort The phototrophic metabolic behaviour of Candidatus accumulibacter
author Carvalho, V. C.F.
author_facet Carvalho, V. C.F.
Gan, A. Z.M.
Shon, A.
Kolakovic, S.
Freitas, E. B.
Reis, M. A.M.
Fradinho, J. C.
Oehmen, A.
author_role author
author2 Gan, A. Z.M.
Shon, A.
Kolakovic, S.
Freitas, E. B.
Reis, M. A.M.
Fradinho, J. C.
Oehmen, A.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
RUN
dc.contributor.author.fl_str_mv Carvalho, V. C.F.
Gan, A. Z.M.
Shon, A.
Kolakovic, S.
Freitas, E. B.
Reis, M. A.M.
Fradinho, J. C.
Oehmen, A.
dc.subject.por.fl_str_mv Candidatus Accumulibacter (Accumulibacter)
Enhanced biological phosphorus removal (EBPR)
Phototrophic growth
Polyphosphate accumulating organisms (PAOs)
Environmental Engineering
Civil and Structural Engineering
Ecological Modelling
Water Science and Technology
Waste Management and Disposal
Pollution
topic Candidatus Accumulibacter (Accumulibacter)
Enhanced biological phosphorus removal (EBPR)
Phototrophic growth
Polyphosphate accumulating organisms (PAOs)
Environmental Engineering
Civil and Structural Engineering
Ecological Modelling
Water Science and Technology
Waste Management and Disposal
Pollution
description Funding Information: The authors also acknowledge the Australian Research Council (ARC) through project LP190100329 . Publisher Copyright: © 2024 The Authors
publishDate 2024
dc.date.none.fl_str_mv 2024-08-01
2024-08-01T00:00:00Z
2025-02-04T21:17:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/178417
url http://hdl.handle.net/10362/178417
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0043-1354
PURE: 106563394
https://doi.org/10.1016/j.watres.2024.121865
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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