The 1.4 Å resolution structure of Paracoccus pantotrophus pseudoazurin

Bibliographic Details
Main Author: Najmudin, Shabir
Publication Date: 2010
Other Authors: Pauleta, Sofia R., Moura, Isabel, Romão, Maria J.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: https://doi.org/10.1107/S1744309110013989
Summary: This work was supported in part by Fundação para a Ciência e a Tecnologia (Lisbon, Portugal) through project grant POCTI/QUI/64638/2006 and individual grant SFRH/BPD/20357/2004 (SN). The authors would like to thank Dr Cecilia Bonifacio for help with crystallization and Dr Jose Trincao for help with data collection.
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spelling The 1.4 Å resolution structure of Paracoccus pantotrophus pseudoazurinCopper proteinsElectron transferParacoccus pantotrophusPseudoazurinType 1 blue copper protein familyBiophysicsStructural BiologyBiochemistryGeneticsCondensed Matter PhysicsThis work was supported in part by Fundação para a Ciência e a Tecnologia (Lisbon, Portugal) through project grant POCTI/QUI/64638/2006 and individual grant SFRH/BPD/20357/2004 (SN). The authors would like to thank Dr Cecilia Bonifacio for help with crystallization and Dr Jose Trincao for help with data collection.Pseudoazurins are small type 1 copper proteins that are involved in the flow of electrons between various electron donors and acceptors in the bacterial periplasm, mostly under denitrifying conditions. The previously determined structure of Paracoccus pantotrophus pseudoazurin in the oxidized form was improved to a nominal resolution of 1.4 Å, with R and R free values of 0.188 and 0.206, respectively. This high-resolution structure makes it possible to analyze the interactions between the monomers and the solvent structure in detail. Analysis of the high-resolution structure revealed the structural regions that are responsible for monomer-monomer recognition during dimer formation and for protein-protein interaction and that are important for partner recognition. The pseudoazurin structure was compared with other structures of various type 1 copper proteins and these were grouped into families according to similarities in their secondary structure; this may be useful in the annotation of copper proteins in newly sequenced genomes and in the identification of novel copper proteins.DQ - Departamento de QuímicaCQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE)RUNNajmudin, ShabirPauleta, Sofia R.Moura, IsabelRomão, Maria J.2019-03-11T23:15:17Z2010-06-102010-06-10T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttps://doi.org/10.1107/S1744309110013989eng1744-3091PURE: 12010412http://www.scopus.com/inward/record.url?scp=77953142481&partnerID=8YFLogxKhttps://doi.org/10.1107/S1744309110013989info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:37:40Zoai:run.unl.pt:10362/62995Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:08:36.524187Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv The 1.4 Å resolution structure of Paracoccus pantotrophus pseudoazurin
title The 1.4 Å resolution structure of Paracoccus pantotrophus pseudoazurin
spellingShingle The 1.4 Å resolution structure of Paracoccus pantotrophus pseudoazurin
Najmudin, Shabir
Copper proteins
Electron transfer
Paracoccus pantotrophus
Pseudoazurin
Type 1 blue copper protein family
Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics
title_short The 1.4 Å resolution structure of Paracoccus pantotrophus pseudoazurin
title_full The 1.4 Å resolution structure of Paracoccus pantotrophus pseudoazurin
title_fullStr The 1.4 Å resolution structure of Paracoccus pantotrophus pseudoazurin
title_full_unstemmed The 1.4 Å resolution structure of Paracoccus pantotrophus pseudoazurin
title_sort The 1.4 Å resolution structure of Paracoccus pantotrophus pseudoazurin
author Najmudin, Shabir
author_facet Najmudin, Shabir
Pauleta, Sofia R.
Moura, Isabel
Romão, Maria J.
author_role author
author2 Pauleta, Sofia R.
Moura, Isabel
Romão, Maria J.
author2_role author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
CQFB-REQUIMTE - Centro de Química Fina e Biotecnologia (Lab. Associado REQUIMTE)
RUN
dc.contributor.author.fl_str_mv Najmudin, Shabir
Pauleta, Sofia R.
Moura, Isabel
Romão, Maria J.
dc.subject.por.fl_str_mv Copper proteins
Electron transfer
Paracoccus pantotrophus
Pseudoazurin
Type 1 blue copper protein family
Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics
topic Copper proteins
Electron transfer
Paracoccus pantotrophus
Pseudoazurin
Type 1 blue copper protein family
Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics
description This work was supported in part by Fundação para a Ciência e a Tecnologia (Lisbon, Portugal) through project grant POCTI/QUI/64638/2006 and individual grant SFRH/BPD/20357/2004 (SN). The authors would like to thank Dr Cecilia Bonifacio for help with crystallization and Dr Jose Trincao for help with data collection.
publishDate 2010
dc.date.none.fl_str_mv 2010-06-10
2010-06-10T00:00:00Z
2019-03-11T23:15:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1107/S1744309110013989
url https://doi.org/10.1107/S1744309110013989
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1744-3091
PURE: 12010412
http://www.scopus.com/inward/record.url?scp=77953142481&partnerID=8YFLogxK
https://doi.org/10.1107/S1744309110013989
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9
application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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