Determining the topology of peroxisomal proteins using protease protection assays

Bibliographic Details
Main Author: Francisco, T
Publication Date: 2017
Other Authors: Dias, AF, Pedrosa, AG, Grou, CP, Rodrigues, TA, Azevedo, JE
Format: Book
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: https://repositorio-aberto.up.pt/handle/10216/117911
Summary: Protease protection assays are powerful tools to determine the topology of organelle proteins. Their simplicity, together with the fact that they are particularly suited to characterize endogenous proteins, are their major advantages and the reason why these assays have been in use for so many years. Here, we provide a detailed protocol to use with mammalian peroxisomes. Suggestions on how these assays can be controlled, and how to identify some technical pitfalls, are also presented.
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spelling Determining the topology of peroxisomal proteins using protease protection assaysProtease protection assays are powerful tools to determine the topology of organelle proteins. Their simplicity, together with the fact that they are particularly suited to characterize endogenous proteins, are their major advantages and the reason why these assays have been in use for so many years. Here, we provide a detailed protocol to use with mammalian peroxisomes. Suggestions on how these assays can be controlled, and how to identify some technical pitfalls, are also presented.Humana Press20172017-01-01T00:00:00Z2019-04-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookapplication/pdfhttps://repositorio-aberto.up.pt/handle/10216/117911eng1064-374510.1007/978-1-4939-6937-1_3Francisco, TDias, AFPedrosa, AGGrou, CPRodrigues, TAAzevedo, JEinfo:eu-repo/semantics/embargoedAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-02-27T16:33:27Zoai:repositorio-aberto.up.pt:10216/117911Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T21:46:37.443914Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Determining the topology of peroxisomal proteins using protease protection assays
title Determining the topology of peroxisomal proteins using protease protection assays
spellingShingle Determining the topology of peroxisomal proteins using protease protection assays
Francisco, T
title_short Determining the topology of peroxisomal proteins using protease protection assays
title_full Determining the topology of peroxisomal proteins using protease protection assays
title_fullStr Determining the topology of peroxisomal proteins using protease protection assays
title_full_unstemmed Determining the topology of peroxisomal proteins using protease protection assays
title_sort Determining the topology of peroxisomal proteins using protease protection assays
author Francisco, T
author_facet Francisco, T
Dias, AF
Pedrosa, AG
Grou, CP
Rodrigues, TA
Azevedo, JE
author_role author
author2 Dias, AF
Pedrosa, AG
Grou, CP
Rodrigues, TA
Azevedo, JE
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Francisco, T
Dias, AF
Pedrosa, AG
Grou, CP
Rodrigues, TA
Azevedo, JE
description Protease protection assays are powerful tools to determine the topology of organelle proteins. Their simplicity, together with the fact that they are particularly suited to characterize endogenous proteins, are their major advantages and the reason why these assays have been in use for so many years. Here, we provide a detailed protocol to use with mammalian peroxisomes. Suggestions on how these assays can be controlled, and how to identify some technical pitfalls, are also presented.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
2019-04-14T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/book
format book
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio-aberto.up.pt/handle/10216/117911
url https://repositorio-aberto.up.pt/handle/10216/117911
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1064-3745
10.1007/978-1-4939-6937-1_3
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Humana Press
publisher.none.fl_str_mv Humana Press
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833599419426013184

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